|
|
Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
01/11/2023 |
Data da última atualização: |
01/11/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DUARTE, G. S.; GALINDO, D. J.; BALDINI, M. H. M.; FONSECA, J. F. da; DUARTE, J. M. B.; OLIVEIRA, M. E. F. |
Afiliação: |
GABRIELLA SALONI DUARTE, Universidade Estadual Paulista; DAVID JAVIER GALINDO, Universidade Estadual Paulista; Universidad Nacional Mayor de San Marcos; MARIA HELENA MAZZONI BALDINI, Universidade Estadual Paulista; JEFERSON FERREIRA DA FONSECA, CNPC; JOSÉ MAURICIO BARBANTI DUARTE, Universidade Estadual Paulista; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista. |
Título: |
Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): a promising method for assisted reproduction in deer. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Scientific Reports, v. 13, n. 1, article 17369, Oct. 2023. |
DOI: |
10.1038/s41598-023-43392-4 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 h after estrus onset. All females received either an i.v. application of 50 IU of OT (G-OT, n = 4) or 1 mL of saline solution (G-Control, n = 4) 20 min before the TCAI procedure. The TCAIs were performed using frozen-thawed semen motility 40%, vigor 3, acrosome integrity 87%, membrane integrity of 95% and 13% of total post-thaw defects from the same batch. Behavioral estrus was observed in 100% of the females, in both replicates. It was achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 min) and TCAI procedures (~ 17 min) were considered satisfactory. Thus, the performance of the TCAI-CI and TCAI-CT techniques was successful, regardless of using OT as a cervical dilation protocol. This procedure is proposed as a method of choice for artificial insemination with greater applicability in different conservation centers, compared to more advanced reproductive biotechniques, and with a favorable impact on the conservation of deer species. MenosAbstract: The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 h after estrus onset. All females received either an i.v. application of 50 IU of OT (G-OT, n = 4) or 1 mL of saline solution (G-Control, n = 4) 20 min before the TCAI procedure. The TCAIs were performed using frozen-thawed semen motility 40%, vigor 3, acrosome integrity 87%, membrane integrity of 95% and 13% of total post-thaw defects from the same batch. Behavioral estrus was observed in 100% of the females, in both replicates. It was achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 min) and TCAI procedures (~ 17 min) were considered satisfactory. Thus, the performance of the TCAI-CI and TCAI-CT techniques was successful, regardless of using OT as a cervical dilation protocol. Thi... Mostrar Tudo |
Palavras-Chave: |
Cervix Uteri; Semen preservation. |
Thesaurus Nal: |
Artificial insemination; Cross-over studies; Deer; Females; Males; Oxytocin; Uterus. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1157668/1/CNPC-2023-Transcervical-artificial-insemination.pdf
|
Marc: |
LEADER 02668naa a2200301 a 4500 001 2157668 005 2023-11-01 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1038/s41598-023-43392-4$2DOI 100 1 $aDUARTE, G. S. 245 $aTranscervical artificial insemination in the brown brocket deer (Subulo gouazoubira)$ba promising method for assisted reproduction in deer.$h[electronic resource] 260 $c2023 520 $aAbstract: The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 h after estrus onset. All females received either an i.v. application of 50 IU of OT (G-OT, n = 4) or 1 mL of saline solution (G-Control, n = 4) 20 min before the TCAI procedure. The TCAIs were performed using frozen-thawed semen motility 40%, vigor 3, acrosome integrity 87%, membrane integrity of 95% and 13% of total post-thaw defects from the same batch. Behavioral estrus was observed in 100% of the females, in both replicates. It was achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 min) and TCAI procedures (~ 17 min) were considered satisfactory. Thus, the performance of the TCAI-CI and TCAI-CT techniques was successful, regardless of using OT as a cervical dilation protocol. This procedure is proposed as a method of choice for artificial insemination with greater applicability in different conservation centers, compared to more advanced reproductive biotechniques, and with a favorable impact on the conservation of deer species. 650 $aArtificial insemination 650 $aCross-over studies 650 $aDeer 650 $aFemales 650 $aMales 650 $aOxytocin 650 $aUterus 653 $aCervix Uteri 653 $aSemen preservation 700 1 $aGALINDO, D. J. 700 1 $aBALDINI, M. H. M. 700 1 $aFONSECA, J. F. da 700 1 $aDUARTE, J. M. B. 700 1 $aOLIVEIRA, M. E. F. 773 $tScientific Reports$gv. 13, n. 1, article 17369, Oct. 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 30 | |
21. | | KAWANAMI, A. E.; OLIVEIRA, J. P. de; ARENALES, A.; CROSSLEY, B.; WOODS, L. W.; DUARTE, J. M. B.; WERTHER, K. Detection of bluetongue virus in Brazilian cervids in São Paulo state. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 137-142, janeiro 2018. Título em português: Detecção de vírus da língua azul em cervídeos brasileiros no estado de São Paulo.Biblioteca(s): Embrapa Unidades Centrais. |
| |
23. | | ANDRIOLO, A.; PIOVEZAN, U.; COSTA, M. J. R. P. da; TORRES, H. A.; VIGLIOTTI, A.; ZERBINI, A. N.; DUARTE, J. M. B. Severe population decline of marsh deer, Blastocerus dichotomus (Cetartiodactyla: Cervidae), a threatened species, caused by flooding related to a hydroelectric power plant. Zoologia, v. 30, n. 6, p. 630-638, 2013. 9 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pantanal. |
| |
24. | | GROTTA NETO, F.; PERES, P. H. de F.; POLVERINI, M. S.; PIOVEZAN, U.; PASSOS, F. de C.; DUARTE, J. M. B. Troca de chifres de veado-catingueiro (Mazama gouazoubira) no Pantanal. In: CONGRESSO BRASILEIRO DE MASTOZOOLOGIA, 7., 2014, Gramado, RS. Caderno de Resumos... Gramado: UFRGS, 2014. SBMZ, 2014. p. 120-121. 2 p.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pantanal. |
| |
25. | | DUARTE, G. S.; GALINDO, D. J.; BALDINI, M. H. M.; FONSECA, J. F. da; DUARTE, J. M. B.; OLIVEIRA, M. E. F. Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): a promising method for assisted reproduction in deer. Scientific Reports, v. 13, n. 1, article 17369, Oct. 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
26. | | PIOVEZAN, U.; ANDRIOLO, A.; TORRES, H. A.; LEMES, M. R. S.; RAMOS, H. G. C.; COSTA, M. J. R. P.; DUARTE, J. M. B. Home range and survival of marsh deer Blastocerus dichotomus (Mammalia: cervidae): the artifical flooding for hydroeléctric power production. In: ANNUAL MEETING OF THE SOCIETY FOR CONSERVATION BIOLOGY, 19., 2005, Brasília, DF. Abstracts... Brasília, DF: University of Brasília, 2005. p.165-166.Biblioteca(s): Embrapa Pantanal. |
| |
27. | | LEMES, M. R. S.; ANDRIOLO, A.; PIOVEZAN, U.; TORRES, H. A.; RAMOS, H. G. da C.; COSTA, M. J. R. P. da; DUARTE, J. M. B. Implantação de sítios temporários de mucuna-preta (Stilozobium aterrimum), em área de impacto, como fonte alternativa de forragem para cervos-do-pantanal (Blastocerus dichotomus (Illiger, 1815)(Mammalia, cervidae). In: CONGRESSO BRASILEIRO DE MASTOZOOLOGIA, 2., 2003, Belo Horizonte, MG. Resumos... Belo Horizonte: PUC Minas, 2003. não paginado.Biblioteca(s): Embrapa Pantanal. |
| |
28. | | LEMES, M. R. S.; ANDRIOLO, A.; PIOVEZAN, U.; TORRES, H. A.; RAMOS, H. G. da C.; COSTA, M. J. R. P. da; DUARTE, J. M. B. Predação de cervo-do-pantanal Blastocerus dichotomus (Illiger, 1815) (Mammalia, cervidae) por onça-parda Puma concolor (Linnaeus, 1771) (Mammalia, felidae) no entorno do reservatório da Usina Hidrelétrica "Sergio Mota", Bacia do Rio Paraná. In: CONGRESSO BRASILEIRO DE MASTOZOOLOGIA, 2., 2003, Belo Horizonte, MG. Resumos... Belo Horizonte: PUC Minas, 2003. p.292.Biblioteca(s): Embrapa Pantanal. |
| |
29. | | COSTA, E. B. V.; OLIVEIRA, M. L. de; PERES, P. H. de F.; GROTTA NETO, F.; VOGLIOTTI, A.; PIOVEZAN, U.; DUARTE, J. M. B. Low accuracy of identifying Neotropical deer species by scat morphology. Studies on Neotropical Fauna and Environment, v. 52, n.1, p. 37-42, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pantanal. |
| |
30. | | DUARTE, J. M. B.; PIOVEZAN, U.; ZANETI, E. dos S.; RAMOS, H. G. da C.; TIEPOLO, L. M.; VOGLIOTTI, A.; OLIVEIRA, M. L. de; RODRIGUES, L. F.; ALMEIDA, L. B. de. Blastocerus dichotomus (Illger, 1815). In: INSTITUTO CHICO MENDES DE CONSERVAÇÃO DA BIODIVERSIDADE. Livro vermelho da fauna brasileira ameaçada de extinção: volume VII - invertebrados. Brasília, DF, 2018. p. 68-73.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
| |
Registros recuperados : 30 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|