Data/hora: 25/06/2024 06:07:43

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	10/01/2018	
Data da última atualização: 	27/01/2023	
Autoria: 	ASSIS, A. S. F.; OTENIO, M. H.; DRUMOND, B. P.; FUMIAN, T. M.; MIAGOSTOVICH, M. P.; SILVA, M. L. da R. e.	
Afiliação: 	Andrêssa S.F.Assis, UFJF; MARCELO HENRIQUE OTENIO, CNPGL; Betânia P. Drumond, UFJF; Tulio M. Fumian, Instituto Oswaldo Cruz; Marize P Miagostovich, Instituto Oswaldo Cruz; Maria Luzia da Rosa e Silva, UFJF.	
Título: 	Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Science of The Total Environment, v. 583, p. 163-168, 2017.	
Idioma: 	Inglês	
Conteúdo: 	Abstract Return of treated sludge to the environment poses concerns and has stimulated the development of studies on viral monitoring in this matrix, in order to assess its potential risks for public health. Human adenovirus (HAdV) has been identified as a putative viral marker of faecal contamination due to its stability and resistance to the sewage treatment process. The aim of this study was to optimize the organic flocculation procedure in order to establish an appropriate methodology for HAdV recovery from sewage sludge samples. Four protocols (A-D) have been proposed, with changes in the initial sample dilution, in the stirring time and in the final concentration of skimmed-milk. A single sludge sample was obtained in Wastewater Treatment Plant (WWTP) and divided into aliquots. In each protocol, three aliquots were inoculated with HAdV and bacteriophage PP7 and a non-inoculated one was used as negative control. Viral load and recovery rate were determined by quantitative PCR. HAdV recovery rate varied between the protocols tested (p=0.016) and the best result was obtained through the protocol C. In order to confirm this result a field study with activated, thickened and digested sludge sampleswas carried out. Different types of sludgewere obtained in twoWWTPs and processed using protocol C. HAdVwas detected in all samples, with a similar or higher viral load than those obtainedwith other concentration techniques already applied to sludge. Protocol C proved to be really efficient, with the advantage of showing low cost and practicability in routine laboratories.	
Palavras-Chave: 	Human adenovirus; Internal control; Organic flocculation; Quantitative PCR; Wastewater treatment plant.	
Thesaurus Nal: 	sewage sludge.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (23856-1) - UPC - AP - DD -  -  -  - DIS