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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
13/12/2021 |
Data da última atualização: |
28/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
HORIKOSHI, R. J.; BERNARDI, O.; SEMEÃO, A. A.; WILLSE, A.; CORAZZA, G. O.; RUTHES, E.; FERNANDES, D. de S.; SÓSA-GOMEZ, D. R.; BUENO, A. de F.; OMOTO, C.; BERGER, G. U.; CORRÊA, A. S.; MARTINELLI, S.; DOURADO, P. M.; HEAD, G. |
Afiliação: |
RENATO J. HORIKOSHI, Bayer Crop Science, Santa Cruz das Palmeiras, SP.; ODERLEI BERNARDI, Universidade Federal de Santa Maria, UFSM, Santa Maria, RS.; ALTAIR A. SEMEÃO, Bayer Crop Science, Santa Cruz das Palmeiras, SP.; ALAN WILLSE, Regulatory Science, Bayer Crop Science US, Chesterfield, MO, USA.; GUSTAVO O. CORAZZA, Bayer Crop Science, Santa Cruz das Palmeiras, SP.; ELDERSON RUTHES, Fundação ABC, Castro, PR.; DAVI DE S. FERNANDES, Escola Superior de Agricultura ?Luiz de Queiroz?, Esalq, Universidade de São Paulo, Piracicaba, SP.; DANIEL RICARDO SOSA GOMEZ, CNPSO; ADENEY DE FREITAS BUENO, CNPSO; CELSO OMOTO, Escola Superior de Agricultura “Luiz de Queiroz”, Esalq, Universidade de São Paulo, Piracicaba, SP.; GERALDO U. BERGER, Bayer Crop Science, São Paulo, SP.; ALBERTO S. CORRÊA, Escola Superior de Agricultura “Luiz de Queiroz”, Esalq, Universidade de São Paulo, Piracicaba, SP.; SAMUEL MARTINELLI, Regulatory Science, Bayer Crop Science US, Chesterfield, MO, USA.; PATRICK M. DOURADO, Bayer Crop Science, São Paulo, SP.; GRAHAM HEAD, Regulatory Science, Bayer Crop Science US, Chesterfield, MO, USA. |
Título: |
Resistance status of lepidopteran soybean pests following large-scale use of MON 87701 × MON 89788 soybean in Brazil. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientific Reports, v. 11, 21323, 2021. |
Páginas: |
15 p. |
DOI: |
https://doi.org/10.1038/s41598-021-00770-0 |
Idioma: |
Inglês |
Thesagro: |
Lepidóptero; Praga; Resistência; Soja. |
Thesaurus Nal: |
Brazil; Lepidoptera; Pest resistance; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01107naa a2200397 a 4500 001 2137703 005 2022-03-28 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1038/s41598-021-00770-0$2DOI 100 1 $aHORIKOSHI, R. J. 245 $aResistance status of lepidopteran soybean pests following large-scale use of MON 87701 × MON 89788 soybean in Brazil.$h[electronic resource] 260 $c2021 300 $a15 p. 650 $aBrazil 650 $aLepidoptera 650 $aPest resistance 650 $aSoybeans 650 $aLepidóptero 650 $aPraga 650 $aResistência 650 $aSoja 700 1 $aBERNARDI, O. 700 1 $aSEMEÃO, A. A. 700 1 $aWILLSE, A. 700 1 $aCORAZZA, G. O. 700 1 $aRUTHES, E. 700 1 $aFERNANDES, D. de S. 700 1 $aSÓSA-GOMEZ, D. R. 700 1 $aBUENO, A. de F. 700 1 $aOMOTO, C. 700 1 $aBERGER, G. U. 700 1 $aCORRÊA, A. S. 700 1 $aMARTINELLI, S. 700 1 $aDOURADO, P. M. 700 1 $aHEAD, G. 773 $tScientific Reports$gv. 11, 21323, 2021.
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| Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
09/05/2012 |
Data da última atualização: |
20/03/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MAIA, M. da S.; BICUDO, S. D.; AZEVEDO, H. C.; SICHERLE, C. C.; SOUSA, D. B. de; RODELLO, L. |
Afiliação: |
MARCIANE DA SILVA MAIA, CPATSA; SONY DIMAS BICUDO, USP; HYMERSON COSTA AZEVEDO; CARMEN CECILIA SICHERLE; DANIEL BARTOLI DE SOUSA; LEANDRO RODELLO, USP. |
Título: |
Motility and viability of ram sperm cryopreserved in a Tris-egg yolk extender supplemented with anti-oxidants. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Small Ruminant Research, v. 85, p. 85-90, 2009. |
DOI: |
10.1016/j.smallrumres.2009.07.001 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to evaluate the effects of the addition of the anti-oxidants Trolox and catalase to a ram semen cryopreservation extender, on the basis of post-thaw sperm motility, plasma membrane integrity, acrosomal membrane integrity and mitochondrial membrane potential. Semen from 23 Santa Inês rams was collected using an artificial vagina, and one ejaculate from each ram was evaluated and included in the study. Semen sampleswere diluted to a concentration of 400×106 cells/ml in Tris-egg yolk extender (control) or the same extender supplemented with 50M Trolox/108 sperm (TRO), 50g catalase/ml (CAT) or a combination of Trolox and catalase (TROCAT). The semen was loaded into 0.25 ml straws, cooled and frozen in a programmable freezer and subsequently stored in liquid nitrogen. Frozen straws were thawed in a water bath for sperm evaluation. Post-thaw sperm motility characteristics were determined by CASA, and plasma membrane integrity, acrosome integrity and mitochondrial membrane potential were assessed using PI/FITC-PSA/JC-1 probes. Total sperm motility was significantly higher (P < 0.05) in the control (72.0±1.5%) and in the samples in which catalase was included in the extender (CAT = 69.0±1.5%), compared to samples containing only Trolox (TRO= 65.0±1.5%) or Trolox in combination with catalase (TROCAT = 61.0±1.5%). Progressive motility was significantly (P < 0.05) higher in the CAT extender (31.0±1.0%) than in the TRO (26.0±1.1%) and TROCAT (27.0±1.1%) supplemented extenders. Catalase supplementation resulted in a significantly (P < 0.05) higher percentage of viable sperm (35.5±1.3%) following cryopreservation, than the control (29.9±1.3%) and TROCAT (28.1±1.3%) supplementation. Anti-oxidant supplementation did not improve mitochondrial activity. The total number of sperm showing a high mitochondrial membrane potential was low in all treatments. The percentage of viable sperm was positively related with total sperm motility, progressive motility and rapid movement, while a negative correlation was observed with the percentage of slow and static sperm. Results indicate that supplementation of the extender with the anti-oxidant catalase improves sperm viability and, to a lesser extent, the progressive motility of ram sperm after cryopreservation in a Tris-egg yolk extender. MenosThe objective of this study was to evaluate the effects of the addition of the anti-oxidants Trolox and catalase to a ram semen cryopreservation extender, on the basis of post-thaw sperm motility, plasma membrane integrity, acrosomal membrane integrity and mitochondrial membrane potential. Semen from 23 Santa Inês rams was collected using an artificial vagina, and one ejaculate from each ram was evaluated and included in the study. Semen sampleswere diluted to a concentration of 400×106 cells/ml in Tris-egg yolk extender (control) or the same extender supplemented with 50M Trolox/108 sperm (TRO), 50g catalase/ml (CAT) or a combination of Trolox and catalase (TROCAT). The semen was loaded into 0.25 ml straws, cooled and frozen in a programmable freezer and subsequently stored in liquid nitrogen. Frozen straws were thawed in a water bath for sperm evaluation. Post-thaw sperm motility characteristics were determined by CASA, and plasma membrane integrity, acrosome integrity and mitochondrial membrane potential were assessed using PI/FITC-PSA/JC-1 probes. Total sperm motility was significantly higher (P < 0.05) in the control (72.0±1.5%) and in the samples in which catalase was included in the extender (CAT = 69.0±1.5%), compared to samples containing only Trolox (TRO= 65.0±1.5%) or Trolox in combination with catalase (TROCAT = 61.0±1.5%). Progressive motility was significantly (P < 0.05) higher in the CAT extender (31.0±1.0%) than in the TRO (26.0±1.1%) and TROCAT (27.0±1.1%) s... Mostrar Tudo |
Palavras-Chave: |
Anti-oxidantes; Plasma membrane integrity; Ram semen; Sêmen ovino. |
Thesagro: |
Esperma; Ovino; Produção animal. |
Thesaurus NAL: |
Animal production. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03206naa a2200289 a 4500 001 1924155 005 2015-03-20 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1016/j.smallrumres.2009.07.001$2DOI 100 1 $aMAIA, M. da S. 245 $aMotility and viability of ram sperm cryopreserved in a Tris-egg yolk extender supplemented with anti-oxidants.$h[electronic resource] 260 $c2009 520 $aThe objective of this study was to evaluate the effects of the addition of the anti-oxidants Trolox and catalase to a ram semen cryopreservation extender, on the basis of post-thaw sperm motility, plasma membrane integrity, acrosomal membrane integrity and mitochondrial membrane potential. Semen from 23 Santa Inês rams was collected using an artificial vagina, and one ejaculate from each ram was evaluated and included in the study. Semen sampleswere diluted to a concentration of 400×106 cells/ml in Tris-egg yolk extender (control) or the same extender supplemented with 50M Trolox/108 sperm (TRO), 50g catalase/ml (CAT) or a combination of Trolox and catalase (TROCAT). The semen was loaded into 0.25 ml straws, cooled and frozen in a programmable freezer and subsequently stored in liquid nitrogen. Frozen straws were thawed in a water bath for sperm evaluation. Post-thaw sperm motility characteristics were determined by CASA, and plasma membrane integrity, acrosome integrity and mitochondrial membrane potential were assessed using PI/FITC-PSA/JC-1 probes. Total sperm motility was significantly higher (P < 0.05) in the control (72.0±1.5%) and in the samples in which catalase was included in the extender (CAT = 69.0±1.5%), compared to samples containing only Trolox (TRO= 65.0±1.5%) or Trolox in combination with catalase (TROCAT = 61.0±1.5%). Progressive motility was significantly (P < 0.05) higher in the CAT extender (31.0±1.0%) than in the TRO (26.0±1.1%) and TROCAT (27.0±1.1%) supplemented extenders. Catalase supplementation resulted in a significantly (P < 0.05) higher percentage of viable sperm (35.5±1.3%) following cryopreservation, than the control (29.9±1.3%) and TROCAT (28.1±1.3%) supplementation. Anti-oxidant supplementation did not improve mitochondrial activity. The total number of sperm showing a high mitochondrial membrane potential was low in all treatments. The percentage of viable sperm was positively related with total sperm motility, progressive motility and rapid movement, while a negative correlation was observed with the percentage of slow and static sperm. Results indicate that supplementation of the extender with the anti-oxidant catalase improves sperm viability and, to a lesser extent, the progressive motility of ram sperm after cryopreservation in a Tris-egg yolk extender. 650 $aAnimal production 650 $aEsperma 650 $aOvino 650 $aProdução animal 653 $aAnti-oxidantes 653 $aPlasma membrane integrity 653 $aRam semen 653 $aSêmen ovino 700 1 $aBICUDO, S. D. 700 1 $aAZEVEDO, H. C. 700 1 $aSICHERLE, C. C. 700 1 $aSOUSA, D. B. de 700 1 $aRODELLO, L. 773 $tSmall Ruminant Research$gv. 85, p. 85-90, 2009.
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