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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
04/01/2016 |
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Data da última atualização: |
25/01/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VOORT, M. van der; MEIJER, H. J. G.; SCHMIDT, Y.; WATROUS, J; DEKKERS, E.; MENDES, R.; DORRESTEIN, P. C; GROSS, H.; RAAIJMAKERS, J. M. |
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Afiliação: |
MENNO VAN DER VOORT, Wageningen University; HAROLD J G MEIJER, Wageningen University; YVONNE SCHMIDT, University of Bonn; JERAMIE WATROUS, University of California; ESTER DEKKERS, Wageningen University; RODRIGO MENDES, CNPMA; PIETER C DORRESTEIN, University of California; HARALD GROSS, University of California; JOS M RAAIJMAKERS, Wageningen University. |
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Título: |
Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 696. |
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Páginas: |
14 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum. MenosAbstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results sh... Mostrar Tudo |
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Palavras-Chave: |
Biocontrol; Genomes sequencing; Pseudomonads. |
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Thesagro: |
Bactéria; Beterraba; Controle biológico; Peptídeo; Pseudomonas sp; Rizosfera. |
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Thesaurus Nal: |
Antimicrobial peptides; Beneficial microorganisms; Biological control; Mass spectrometry; Rhizosphere bacteria; Sequence analysis. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136527/1/2015AP29.pdf
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Marc: |
LEADER 02843naa a2200409 a 4500
001 2032807
005 2016-01-25
008 2015 bl uuuu u00u1 u #d
100 1 $aVOORT, M. van der
245 $aGenome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds.$h[electronic resource]
260 $c2015
300 $a14 p.
520 $aAbstract: The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum.
650 $aAntimicrobial peptides
650 $aBeneficial microorganisms
650 $aBiological control
650 $aMass spectrometry
650 $aRhizosphere bacteria
650 $aSequence analysis
650 $aBactéria
650 $aBeterraba
650 $aControle biológico
650 $aPeptídeo
650 $aPseudomonas sp
650 $aRizosfera
653 $aBiocontrol
653 $aGenomes sequencing
653 $aPseudomonads
700 1 $aMEIJER, H. J. G.
700 1 $aSCHMIDT, Y.
700 1 $aWATROUS, J
700 1 $aDEKKERS, E.
700 1 $aMENDES, R.
700 1 $aDORRESTEIN, P. C
700 1 $aGROSS, H.
700 1 $aRAAIJMAKERS, J. M.
773 $tFrontiers in Microbiology, Lausanne$gv. 6, 2015. Article 696.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 3 | |
| 2. |  | VOORT, M. van der; MEIJER, H. J. G.; SCHMIDT, Y.; WATROUS, J; DEKKERS, E.; MENDES, R.; DORRESTEIN, P. C; GROSS, H.; RAAIJMAKERS, J. M. Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds. Frontiers in Microbiology, Lausanne, v. 6, 2015. Article 696. 14 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 3. | | ZUFFA, S.; SCHMID, R.; BAUERMEISTER, A.; GOMES, P. W. P.; CARABALLO-RODRIGUEZ, A. M.; EL ABIEAD, Y.; ARON, A. T.; GENTRY, E. C.; ZEMLIN, J.; MEEHAN, M. J.; AVALON, N. E.; CICHEWICZ, R. H.; BUZUN, E.; CARRILLO TERRAZAS, M.; HSU, C. Y.; OLES, R.; AYALA, A. V.; ZHAO, J.; CHU, H.; KUIJPERS, M. C. M.; JACKREL, S. L.; TUGIZIMANA, F.; NEPHALI, L. P.; DUBERY, I. A.; MADALA, N. E.; MOREIRA, E. A.; COSTA-LOTUFO, L. V.; LOPES, N. P.; REZENDE-TEIXEIRA, P.; JIMENEZ, P. C.; RIMAL, B.; PATTERSON, A. D.; TRAXLER, M. F.; PESSOTTI, R. de C.; ALVARADO-VILLALOBOS, D.; TAMAYO-CASTILLO, G.; CHAVERRI, P.; ESCUDERO-LEYVA, E.; QUIROS-GUERRERO, L. M.; BORY, A. J.; JOUBERT, J.; RUTZ, A.; WOLFENDER, J. L.; ALLARD, P. M.; SICHERT, A.; PONTRELLI, S.; PULLMAN, B. S.; BANDEIRA, N.; GERWICK, W. H.; GINDRO, K.; MASSANA-CODINA, J.; WAGNER, B. C.; FORCHHAMMER, K.; PETRAS, D.; AIOSA, N.; GARG, N.; LIEBEKE, M.; BOURCEAU, P.; KANG, K. B.; GADHAVI, H.; CARVALHO, L. P. S. de; SANTOS, M. S. dos; PÉREZ-LORENTE, A. I.; MOLINA-SANTIAGO, C.; ROMERO, D.; FRANKE, R.; BRÖNSTRUP, M.; PONCE DE LEÓN, A. V.; POPE, P. B.; LA ROSA, S. L.; LA BARBERA, G.; ROAGER, H. M.; LAURSEN, M. F.; HAMMERLE, F.; SIEWERT, B.; PEINTNER, U.; LICONA-CASSANI, C.; RODRIGUEZ-ORDUÑA, L.; RAMPLER, E.; HILDEBRAND, F.; KOELLENSPERGER, G.; SCHOENY, H.; HOHENWALLNER, K.; PANZENBOECK, L.; GREGOR, R.; O'NEILL, E. C.; ROXBOROUGH, E. T.; ODOI, J.; BALE, N. J.; DING, S.; DAMSTÉ, J. S. S.; GUAN, X. L.; CUI, J. J.; JU, K. S.; SILVA, D. B.; SILVA, F. M. R.; SILVA, G. F. da; KOOLEN, H. H. F.; GRUNDMANN, C.; CLEMENT, J. A.; MOHIMANI, H.; BRODERS, K.; McPHAIL, K. L.; OBER-SINGLETON, S. E.; RATH, C. M.; McDONALD, D.; KNIGHT, R.; WANG, M.; DORRESTEIN, P. C. MicrobeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data. Nature Microbiology, v. 9, p. 336-345, Feb. 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 3 | |
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