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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/03/2016 |
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Data da última atualização: |
23/03/2016 |
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Autoria: |
DONIN, D. G.; LEME, R. de A.; ALFIERI, A. F.; ALBERTON, G. C.; ALFIERI, A. A. |
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Afiliação: |
DAIANE G. DONIN, UFPR; RAQUEL DE A LEME, UEL; ALICE F. ALFIERI, UEL; GERALDO C. ALBERTON, UFPR; AMAURI A. ALFIERI, UFPR. |
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Título: |
Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná State, Brazil. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 403-408, maio 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric icornaviruses in wild boars (Susscrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5?non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87.1% to 100% for EV-G. Peccaries tested negative for the viruses and in this study they did not represent infection reservoirs. This study is the first to report the molecular detection of PTV, PSV, and EV-G from captive wild boars in a South American country and the first to screen peccaries as alternative host species for porcine enteric picornavirus. MenosPorcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric icornaviruses in wild boars (Susscrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5?non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, an... Mostrar Tudo |
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Palavras-Chave: |
Pecari tajacu; PTV; Sapelovírus suíno; Sus scrofa scrofa; Tayassu pecari; Teschovírus suíno. |
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Thesagro: |
Javali. |
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Thesaurus Nal: |
Enterovirus G; Porcine sapelovirus; Porcine teschovirus; Wild boars. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141601/1/Molecular-survey-of-porcine.pdf
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Marc: |
LEADER 02778naa a2200301 a 4500
001 2041706
005 2016-03-23
008 2015 bl uuuu u00u1 u #d
100 1 $aDONIN, D. G.
245 $aMolecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná State, Brazil.
260 $c2015
520 $aPorcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric icornaviruses in wild boars (Susscrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5?non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87.1% to 100% for EV-G. Peccaries tested negative for the viruses and in this study they did not represent infection reservoirs. This study is the first to report the molecular detection of PTV, PSV, and EV-G from captive wild boars in a South American country and the first to screen peccaries as alternative host species for porcine enteric picornavirus.
650 $aEnterovirus G
650 $aPorcine sapelovirus
650 $aPorcine teschovirus
650 $aWild boars
650 $aJavali
653 $aPecari tajacu
653 $aPTV
653 $aSapelovírus suíno
653 $aSus scrofa scrofa
653 $aTayassu pecari
653 $aTeschovírus suíno
700 1 $aLEME, R. de A.
700 1 $aALFIERI, A. F.
700 1 $aALBERTON, G. C.
700 1 $aALFIERI, A. A.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 35, n. 5, p. 403-408, maio 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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| 1. |  | DONIN, D. G.; LEME, R. de A.; ALFIERI, A. F.; ALBERTON, G. C.; ALFIERI, A. A. Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná State, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 403-408, maio 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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