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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
21/09/2018 |
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Data da última atualização: |
03/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DINATO, N. B.; LEONARDCZ, E.; BURSON, B. L.; QUARÍN, C. L.; PAULA, A. F. de; FAVERO, A. P. |
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Afiliação: |
Naiana Barbosa Dinato, UFSCAR; Eduardo Leonardecz, UFSCAR; Byron L. Burson, USDA-ARS; Camilo L. Quarín, IBONE; Ailton Ferreira de Paula, UFSCAR; ALESSANDRA PEREIRA FAVERO, CPPSE. |
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Título: |
Storage of Bahiagrass Pollen at Different Temperatures. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Crop Science, v. 58, p. 1-8, 2018. |
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DOI: |
10.2135/cropsci2018.03.0164 |
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Idioma: |
Inglês |
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Conteúdo: |
Paspalum is an important genus of grasses, and some species are used for forage in the warmer regions of the world. Efforts to develop improved cultivars often require intra- and interspecific hybridization, but asynchronous flowering sometimes prevents hybridization between parental species. One method of circumventing this problem is to store pollen using cryopreservation so pollen will be available to pollinate the female parent when it flowers. Bahiagrass (Paspalum notatum Flügge) was used to determine if Paspalum pollen can be preserved using long-term cold storage. Pollen was dehydrated with LiCl, MgCl2, NaOH, and silica gel for 30, 60, and 120 min. Both dehydrated and nondehydrated pollen were stored at 4, -20, and 196°C for 10, 60, 120, and 180 d. After thawing, pollen stainability and in vivo germination were used to assess viability. Stainability of fresh pollen was 73.3%, and for treated and stored pollen, it ranged from 25.3 to 71.3%. Storage at -20 and -196°C was superior to storage at 4°C. Pollen dehydrated with LiCl for 30 min stored best at -196°C; pollen dehydrated for 120 min with silica gel and for 30 and 60 min with MgCl2 stored best at -20°C. All samples regardless of treatment, storage temperature, or length of storage had viable pollen. These findings demonstrate that bahiagrass pollen can be stored in longterm cold conditions, and this procedure can be used to circumvent problems associated with asynchronous flowering between parental species used in wide hybridization programs MenosPaspalum is an important genus of grasses, and some species are used for forage in the warmer regions of the world. Efforts to develop improved cultivars often require intra- and interspecific hybridization, but asynchronous flowering sometimes prevents hybridization between parental species. One method of circumventing this problem is to store pollen using cryopreservation so pollen will be available to pollinate the female parent when it flowers. Bahiagrass (Paspalum notatum Flügge) was used to determine if Paspalum pollen can be preserved using long-term cold storage. Pollen was dehydrated with LiCl, MgCl2, NaOH, and silica gel for 30, 60, and 120 min. Both dehydrated and nondehydrated pollen were stored at 4, -20, and 196°C for 10, 60, 120, and 180 d. After thawing, pollen stainability and in vivo germination were used to assess viability. Stainability of fresh pollen was 73.3%, and for treated and stored pollen, it ranged from 25.3 to 71.3%. Storage at -20 and -196°C was superior to storage at 4°C. Pollen dehydrated with LiCl for 30 min stored best at -196°C; pollen dehydrated for 120 min with silica gel and for 30 and 60 min with MgCl2 stored best at -20°C. All samples regardless of treatment, storage temperature, or length of storage had viable pollen. These findings demonstrate that bahiagrass pollen can be stored in longterm cold conditions, and this procedure can be used to circumvent problems associated with asynchronous flowering between parental species used in ... Mostrar Tudo |
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Thesagro: |
Criopreservação; Gramínea; Pólen. |
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Thesaurus Nal: |
Forage grasses; Paspalum. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02209naa a2200253 a 4500
001 2096131
005 2019-01-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.2135/cropsci2018.03.0164$2DOI
100 1 $aDINATO, N. B.
245 $aStorage of Bahiagrass Pollen at Different Temperatures.$h[electronic resource]
260 $c2018
520 $aPaspalum is an important genus of grasses, and some species are used for forage in the warmer regions of the world. Efforts to develop improved cultivars often require intra- and interspecific hybridization, but asynchronous flowering sometimes prevents hybridization between parental species. One method of circumventing this problem is to store pollen using cryopreservation so pollen will be available to pollinate the female parent when it flowers. Bahiagrass (Paspalum notatum Flügge) was used to determine if Paspalum pollen can be preserved using long-term cold storage. Pollen was dehydrated with LiCl, MgCl2, NaOH, and silica gel for 30, 60, and 120 min. Both dehydrated and nondehydrated pollen were stored at 4, -20, and 196°C for 10, 60, 120, and 180 d. After thawing, pollen stainability and in vivo germination were used to assess viability. Stainability of fresh pollen was 73.3%, and for treated and stored pollen, it ranged from 25.3 to 71.3%. Storage at -20 and -196°C was superior to storage at 4°C. Pollen dehydrated with LiCl for 30 min stored best at -196°C; pollen dehydrated for 120 min with silica gel and for 30 and 60 min with MgCl2 stored best at -20°C. All samples regardless of treatment, storage temperature, or length of storage had viable pollen. These findings demonstrate that bahiagrass pollen can be stored in longterm cold conditions, and this procedure can be used to circumvent problems associated with asynchronous flowering between parental species used in wide hybridization programs
650 $aForage grasses
650 $aPaspalum
650 $aCriopreservação
650 $aGramínea
650 $aPólen
700 1 $aLEONARDCZ, E.
700 1 $aBURSON, B. L.
700 1 $aQUARÍN, C. L.
700 1 $aPAULA, A. F. de
700 1 $aFAVERO, A. P.
773 $tCrop Science$gv. 58, p. 1-8, 2018.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 22 | |
| 10. |  | DINATO, N. B.; SANTOS, I. R. I.; LEONARDECZ NETO, E.; PAULA, A. F. de; FÁVERO, A. P. Conservação a longo prazo de grãos de pólen de Paspalum notatum por criopreservação. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. p. 317.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | DINATO, N. B.; SANTOS, I. R. I.; LEONARDECZ NETO, E.; PAULA, A. F. de; FÁVERO, A. P. Conservação a longo prazo de grãos de pólen de Paspalum notatum por criopreservação. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | DINATO, N. B.; DANIEL, N.; PAULA, A. F. de; VIGNA, B. B. Z.; MATTA, F. de P.; FAVERO, A. P. O sucesso do uso de grãos de pólen criopreservados em cruzamentos interespecíficos de espécies assíncronas de Paspalum. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020. Trabalho 265. 09 a 12 de Novembro de 2020.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | FAVERO, A. P.; CUSTODIO, A. R.; DINATO, N. B.; GODOY, I. J. de; SEIJO, J. G.; MICHELOTTO, M. D. Transference of multiple resistance to peanut through the development of cross-compatible complex hybrids of wild Arachis. Genetics and Molecular Biology, v. 43, n. 2, jun. 2020, e20190099.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | DINATO, N. B.; SANTOS, I. R. I.; LEONARDECZ NETO, E.; PAULA, A. F. de; FAVERO, A. P. Viabilidade de grãos de pólen de Paspalum notatum armazenados em nitrogênio líquido. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA PARA O PROGRESSO DA CIÊNCIA, 67., 2015, São Carlos, SP. Resumos....São Paulo: SBPC, 2015 C.8.1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | DINATO, N. B.; SANTOS, I. R. I.; LEONARDECZ, E.; BURSON, B. L.; QUARÍN, C. L.; PAULA, A. F. de; FAVERO, A. P. Storage of bahiagrass pollen at different temperatures. Crop Science, v. 58, n. 6, p. 2391-2398, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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