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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
31/05/2005 |
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Data da última atualização: |
14/08/2024 |
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Autoria: |
DIAS, S. C.; FRANCO, O. L.; MAGALHÃES, C. P.; OLIVEIRA-NETO, O. B. de; ALUMANN, R. A.; FIGUEIRA, E. L. Z.; MELO, F. R.; GROSSI-de-SÁ, M. F. |
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Título: |
Molecular cloning and expression of an alpha- amylase inhibitor from rye with potential for controlling insect pests. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
The Protein Journal, v. 24, n. 2, p. 113-123, Feb. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new -amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of -amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic -amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration. |
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Palavras-Chave: |
Alfa amilase; Clonagem molecular; Proteína CM. |
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Thesagro: |
Defesa Vegetal; Inibidor de Enzima. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02057naa a2200265 a 4500
001 1185701
005 2024-08-14
008 2005 bl uuuu u00u1 u #d
100 1 $aDIAS, S. C.
245 $aMolecular cloning and expression of an alpha- amylase inhibitor from rye with potential for controlling insect pests.$h[electronic resource]
260 $c2005
520 $aAlpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new -amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of -amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic -amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
650 $aDefesa Vegetal
650 $aInibidor de Enzima
653 $aAlfa amilase
653 $aClonagem molecular
653 $aProteína CM
700 1 $aFRANCO, O. L.
700 1 $aMAGALHÃES, C. P.
700 1 $aOLIVEIRA-NETO, O. B. de
700 1 $aALUMANN, R. A.
700 1 $aFIGUEIRA, E. L. Z.
700 1 $aMELO, F. R.
700 1 $aGROSSI-de-SÁ, M. F.
773 $tThe Protein Journal$gv. 24, n. 2, p. 113-123, Feb. 2005.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 62 | |
| 61. |  | SILVA, F. B.; MONTEIRO, A. C. S.; DEL SARTO, R. P.; MARRA, B. M.; DIAS, S. C.; FIGUEIRA, E. L. Z.; OLIVEIRA, G. R.; ROCHA, T. L.; SOUZA, D. S. L.; SILVA, M. C. M. da; FRANCO, O. L.; GROSSI DE SA, M. F. Proregion of Acanthoscelides obtectus cysteine proteinase: a novel peptide with enhanced selectivity toward endogenous enzymes. Peptides, New York, v. 28, p. 1292-1298, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 62. | | NAKASU, E. Y. T.; DIAS, S. C.; TOGNI, P. H. B.; MACEDO, T. R.; AYRES, K. F.; SILVA, I. S. da; JUÁREZ, C. M. J.; SUJII, E. R.; PIRES, C. S. S.; SA, M. F. G. de; FONTES, E. M. G. Protocolo para avaliação do efeito de proteínas inseticidas sobre o predador Cycloneda sanguinea (Coleoptera: Coccinelidae). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 21., 2006, Recife, PE. Entomologia: da academia à transferência de tecnologia: resumos. Recife: SEB: UFRPE, 2006. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 62 | |
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