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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
14/12/2020 |
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Data da última atualização: |
14/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUATRIN, G. D.; PIZZUTTI, I. R.; GEBLER, L.; DIAS, J. V.; CARDOSO, C. D. |
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Afiliação: |
GUSTAVO D. QUATRIN, FEDERAL UNIVERSITY OF SANTA MARIA (UFSM), CHEMISTRY DEPARTMENT, CENTER OF RESEARCH AND ANALYSIS OF RESIDUES AND CONTAMINANTS (CEPARC), 97105-900 SANTA MARIA, RS, BRAZIL; IONARA R. PIZZUTTI, FEDERAL UNIVERSITY OF SANTA MARIA (UFSM), CHEMISTRY DEPARTMENT, CENTER OF RESEARCH AND ANALYSIS OF RESIDUES AND CONTAMINANTS (CEPARC), 97105-900 SANTA MARIA, RS, BRAZIL; LUCIANO GEBLER, CNPUV; JONATAN V. DIAS, FEDERAL UNIVERSITY OF SANTA MARIA (UFSM), CHEMISTRY DEPARTMENT, CENTER OF RESEARCH AND ANALYSIS OF RESIDUES AND CONTAMINANTS (CEPARC), 97105-900 SANTA MARIA, RS, BRAZIL; CARMEM D. CARDOSO, FEDERAL UNIVERSITY OF SANTA MARIA (UFSM), CHEMISTRY DEPARTMENT, CENTER OF RESEARCH AND ANALYSIS OF RESIDUES AND CONTAMINANTS (CEPARC), 97105-900 SANTA MARIA, RS, BRAZIL. |
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Título: |
New analytical method for chlorpyrifos determination in biobeds constructed in Brazil: Development and validation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, v. 1157, p. 122285, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
A quick and efficient method was optimized and validated to determine chlorpyrifos in biobeds using ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Chlorpyrifos was extracted from the matrix with 30 mL of a mixture of acetone, phosphoric acid and water 98:1:1 (v/v/v). After homogenization, centrifugation and filtration, 125 μL of the extract was evaporated and reconstituted in 5 mL of methanol acidified with 0.1% acetic acid. Validation was performed by studying analytical curve linearity (r2), estimated instrument and method limits of detection and limits of quantification (LODi, LODm, LOQi and LOQm, respectively), accuracy, precision (expressed as relative standard deviation, RSD), and matrix effect. Accuracy and precision were determined from the amount of pesticide recovered from biobed blank samples (i.e. without pesticide residue) spiked with chlorpyrifos at three different concentrations (2, 10 and 50 mg kg−1), with seven replicates at each concentration. For all three concentrations studied, the average recovery values obtained were between 96 and 115% with RSD values lower than 20%. The validated LOQ obtained was 2 mg kg−1 (from recovery studies) and the matrix effect observed was lower than ± 20%, which demonstrated that there was neither considerable suppression nor enhancement of the analyte signal. The biobed system efficiently degraded chlorpyrifos in both 1) simulation of accidental spillage and 2) application of diluted pesticide solution. In the latter case, all the values obtained at the final sampling time (14 months) were below the validated LOQm. MenosA quick and efficient method was optimized and validated to determine chlorpyrifos in biobeds using ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Chlorpyrifos was extracted from the matrix with 30 mL of a mixture of acetone, phosphoric acid and water 98:1:1 (v/v/v). After homogenization, centrifugation and filtration, 125 μL of the extract was evaporated and reconstituted in 5 mL of methanol acidified with 0.1% acetic acid. Validation was performed by studying analytical curve linearity (r2), estimated instrument and method limits of detection and limits of quantification (LODi, LODm, LOQi and LOQm, respectively), accuracy, precision (expressed as relative standard deviation, RSD), and matrix effect. Accuracy and precision were determined from the amount of pesticide recovered from biobed blank samples (i.e. without pesticide residue) spiked with chlorpyrifos at three different concentrations (2, 10 and 50 mg kg−1), with seven replicates at each concentration. For all three concentrations studied, the average recovery values obtained were between 96 and 115% with RSD values lower than 20%. The validated LOQ obtained was 2 mg kg−1 (from recovery studies) and the matrix effect observed was lower than ± 20%, which demonstrated that there was neither considerable suppression nor enhancement of the analyte signal. The biobed system efficiently degraded chlorpyrifos in both 1) simulation of accidental spillage and 2) appl... Mostrar Tudo |
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Palavras-Chave: |
Biomixture; Method validation; Solid phase bioreactor; UPLC–MS/MS. |
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Thesaurus Nal: |
Pesticide degradation. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219141/1/1-s2.0-S1570023220302464-main.pdf
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Marc: |
LEADER 02413naa a2200229 a 4500
001 2128061
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
100 1 $aQUATRIN, G. D.
245 $aNew analytical method for chlorpyrifos determination in biobeds constructed in Brazil$bDevelopment and validation.$h[electronic resource]
260 $c2020
520 $aA quick and efficient method was optimized and validated to determine chlorpyrifos in biobeds using ultraperformance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Chlorpyrifos was extracted from the matrix with 30 mL of a mixture of acetone, phosphoric acid and water 98:1:1 (v/v/v). After homogenization, centrifugation and filtration, 125 μL of the extract was evaporated and reconstituted in 5 mL of methanol acidified with 0.1% acetic acid. Validation was performed by studying analytical curve linearity (r2), estimated instrument and method limits of detection and limits of quantification (LODi, LODm, LOQi and LOQm, respectively), accuracy, precision (expressed as relative standard deviation, RSD), and matrix effect. Accuracy and precision were determined from the amount of pesticide recovered from biobed blank samples (i.e. without pesticide residue) spiked with chlorpyrifos at three different concentrations (2, 10 and 50 mg kg−1), with seven replicates at each concentration. For all three concentrations studied, the average recovery values obtained were between 96 and 115% with RSD values lower than 20%. The validated LOQ obtained was 2 mg kg−1 (from recovery studies) and the matrix effect observed was lower than ± 20%, which demonstrated that there was neither considerable suppression nor enhancement of the analyte signal. The biobed system efficiently degraded chlorpyrifos in both 1) simulation of accidental spillage and 2) application of diluted pesticide solution. In the latter case, all the values obtained at the final sampling time (14 months) were below the validated LOQm.
650 $aPesticide degradation
653 $aBiomixture
653 $aMethod validation
653 $aSolid phase bioreactor
653 $aUPLC–MS/MS
700 1 $aPIZZUTTI, I. R.
700 1 $aGEBLER, L.
700 1 $aDIAS, J. V.
700 1 $aCARDOSO, C. D.
773 $tJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences$gv. 1157, p. 122285, 2020.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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| Registros recuperados : 5 | |
| 1. |  | QUATRIN, G. D.; DIAS, J. V.; GEBLER, L.; CARDOSO, C. D. Avaliação da eficiência do sistema Biobed Brasil na degração de clorpirifos. In: WORKSHOP LATINO AMERICANO SOBRE BIOBED, 3., 2017, Bento Gonçalves. Anais...Bento Gonçalves, RS: Embrapa Uva e Vinho, p. 31, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 2. | | QUATRIN, G. D.; DIAS, J. V.; PIZZUTTI, I. R.; GEBLER, L.; CARDOSO, C. D. Development and validation of analytical method for chlorpyrifos determination in biobeds. In: Latin American Pesticide Residue Workshop, 5., 2015, Santiago, Chile. Anais...Santiago, Chile: LAPRW, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | PIZZUTTI, I. R.; VELA, G. M. E.; KOK, A. de; SCHOLTEN, J. M.; DIAS, J. V.; CARDOSO, C. D.; CONCENCO, G.; VIVIAN, R. Determination of paraquat and diquat: LC-MS method optimization and validation. Food Chemistry, v. 209, p. 248-255, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | CARVALHO, N. S. de; CAMARGO, E. V. de; PIRES, C. C.; BORGES, L. D.; NALERIO, E. S.; GUTERRES, A. de S.; DIAS, J. V. Sheep carcass characteristics supplemented with mixture of essential oils. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., 2015, Belo Horizonte. Zootecnia: otimizando recursos e potencialidades: anais. Brasília, DF: Sociedade Brasileira de Zootecnia, 2015. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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