Data/hora: 16/06/2024 00:28:19

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 26/12/2001
Data da última atualização: | 14/01/2020
Autoria: | FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; VIANNA, M. G. R.; ARAGÃO, F. J. L.
Afiliação: | JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen.
Título: | Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.
Ano de publicação: | 2001
Fonte/Imprenta: | In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC, 2001.
Idioma: | Português
Conteúdo: | Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.
Palavras-Chave: | BGMV; Biobalística; Tolerância; Transformação.
Thesagro: | Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus.
Thesaurus Nal: | Bean golden mosaic virus; mutation.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/208710/1/feijoeiro-transgenico0001.pdf
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (18085-1) - UPC - AA - DD - 2001 -  - 2001 - DIS