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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/03/2017 |
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Data da última atualização: |
26/01/2024 |
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Autoria: |
ADDEO, F.; MAURIELLO, R.; DI LUCCIA, A. |
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Afiliação: |
Francesco Addeo; Rosalba Mauriello; Aldo Di Luccia. |
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Título: |
A gel electrophoretic study of caprine casein. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 55, n. 3, p. 413-421, Aug. 1988. |
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DOI: |
https://doi.org/10.1017/S0022029900028661 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: To compare the resolving power of starch-urea gel (SUGE) and polyacrylamide-agarose gel electrophoresis (PAAGE) in caprine casein analysis, polyacrylamide gel isoelectric focusing (PAGIF) was used as reference method. The PAAGE or SUGE patterns in the first dimension were allowed to migrate by PAGIF in an orthogonal direction giving rise to two-dimensional (2-D) separations. Using this procedure, some individual bands considered to be homogeneous by SUGE or PAAGE were found to be complex mixtures of casein components. A detailed analysis of the analytical capabilities of SUGE and PAAGE was performed. Procedures based on the densitometric reading of SUGE and PAAGE patterns seemed to give inaccurate results on the quantitative composition of caprine casein. Among the electrophoretic methods assayed, the 2-D procedure gave the best resolution of casein fractions. |
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Palavras-Chave: |
Eletroforese em gel. |
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Thesagro: |
Caprino; Caseína; Leite de cabra. |
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Thesaurus Nal: |
Casein; Gel electrophoresis; Goat milk. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01557naa a2200241 a 4500
001 2067341
005 2024-01-26
008 1988 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0022029900028661$2DOI
100 1 $aADDEO, F.
245 $aA gel electrophoretic study of caprine casein.$h[electronic resource]
260 $c1988
520 $aAbstract: To compare the resolving power of starch-urea gel (SUGE) and polyacrylamide-agarose gel electrophoresis (PAAGE) in caprine casein analysis, polyacrylamide gel isoelectric focusing (PAGIF) was used as reference method. The PAAGE or SUGE patterns in the first dimension were allowed to migrate by PAGIF in an orthogonal direction giving rise to two-dimensional (2-D) separations. Using this procedure, some individual bands considered to be homogeneous by SUGE or PAAGE were found to be complex mixtures of casein components. A detailed analysis of the analytical capabilities of SUGE and PAAGE was performed. Procedures based on the densitometric reading of SUGE and PAAGE patterns seemed to give inaccurate results on the quantitative composition of caprine casein. Among the electrophoretic methods assayed, the 2-D procedure gave the best resolution of casein fractions.
650 $aCasein
650 $aGel electrophoresis
650 $aGoat milk
650 $aCaprino
650 $aCaseína
650 $aLeite de cabra
653 $aEletroforese em gel
700 1 $aMAURIELLO, R.
700 1 $aDI LUCCIA, A.
773 $tJournal of Dairy Research$gv. 55, n. 3, p. 413-421, Aug. 1988.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
