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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
01/04/1998 |
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Data da última atualização: |
01/04/1998 |
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Autoria: |
PRICE, M.; SCHELL, J.; GROSSER, J.; PAPPU, S. S.; PAPPU, H. R.; FEBRES, V.; MANJUNATH, K. L.; NIBLETT, C. L.; DERRICK, K. S.; LEE, R. F. |
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Título: |
Replication of citrus tristeza closterovirus in Citrus protoplasts. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Phytopathology, v. 86, n. 8, p. 830-833, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
The study of citrus tristeza closterovirus (CTV) has been hindered by particle fragility, the inherently low virus titers, and its woody citrus hosts. We report the development of an in vitro Citrus sinensis cv. Hamlin protoplast system useful for the study of CTV replication. Full-length CTV genomic RNA from purified virions was inoculated into 'Hamlin' protoplasts in the presence of poyethylene glycol. After 24 h, the protoplasts were assayed for virus replication. Positive - and negative-strand CTV genomic RNA were detected by Northern hybridization using digoxigenin-labled RNA probes. Three viral-encoded proteins of 20, 25 (capsid protein), and 27 kDa were detected by Western blotting, demonstrating that the viral RNA was translated in vivo. CTV virus particles also were isolated and observed in the electron microscope. This was the first demonstration of the infectivity of CTV RNA and the first report of CTV replication in protoplasts. This system will enable a more detailed study of the replication strategy of this economically important and complex virus. |
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Palavras-Chave: |
Citro; Citrus protoplasts; Replicacao; Replication; Tristeza. |
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Thesagro: |
Biotecnologia; Doença; Vírus. |
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Thesaurus Nal: |
Citrus; Closterovirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01940naa a2200349 a 4500
001 1053521
005 1998-04-01
008 1996 bl --- 0-- u #d
100 1 $aPRICE, M.
245 $aReplication of citrus tristeza closterovirus in Citrus protoplasts.
260 $c1996
520 $aThe study of citrus tristeza closterovirus (CTV) has been hindered by particle fragility, the inherently low virus titers, and its woody citrus hosts. We report the development of an in vitro Citrus sinensis cv. Hamlin protoplast system useful for the study of CTV replication. Full-length CTV genomic RNA from purified virions was inoculated into 'Hamlin' protoplasts in the presence of poyethylene glycol. After 24 h, the protoplasts were assayed for virus replication. Positive - and negative-strand CTV genomic RNA were detected by Northern hybridization using digoxigenin-labled RNA probes. Three viral-encoded proteins of 20, 25 (capsid protein), and 27 kDa were detected by Western blotting, demonstrating that the viral RNA was translated in vivo. CTV virus particles also were isolated and observed in the electron microscope. This was the first demonstration of the infectivity of CTV RNA and the first report of CTV replication in protoplasts. This system will enable a more detailed study of the replication strategy of this economically important and complex virus.
650 $aCitrus
650 $aClosterovirus
650 $aBiotecnologia
650 $aDoença
650 $aVírus
653 $aCitro
653 $aCitrus protoplasts
653 $aReplicacao
653 $aReplication
653 $aTristeza
700 1 $aSCHELL, J.
700 1 $aGROSSER, J.
700 1 $aPAPPU, S. S.
700 1 $aPAPPU, H. R.
700 1 $aFEBRES, V.
700 1 $aMANJUNATH, K. L.
700 1 $aNIBLETT, C. L.
700 1 $aDERRICK, K. S.
700 1 $aLEE, R. F.
773 $tPhytopathology$gv. 86, n. 8, p. 830-833, 1996.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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