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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
19/06/2018 |
Data da última atualização: |
19/06/2018 |
Autoria: |
MOLINARI, P. C. C.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; BATISTA, C. F.; SOUZA, F. N. |
Afiliação: |
Paula C. C. Molinari, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Maiara G. Blagitz, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Alice M. M. P. Della Libera, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Camila F. Batista, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Fernando N. Souza, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo. |
Título: |
Intracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 659-664, abril 2018 |
Idioma: |
Inglês |
Notas: |
Título em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à mastite. |
Conteúdo: |
The immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity values, which was also confirmed by the Youden index values being far from one. In conclusion, the assessment of milk bovine neutrophil functions could improve our understanding of the cellular basis of mastitis. Although, the intracellular ROS production and S. aureus phagocytosis by milk neutrophil did not have high predictive values to detect intramammary infections, our results strengthen the idea that that poor bovine mammary gland neutrophil phagocytic ability may be associated with high SCC, and might be considered to identify susceptible dairy cows to mastitis. MenosThe immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity v... Mostrar Tudo |
Palavras-Chave: |
Especificidade; Infecção intramamária; Intracellular reactive; Intramammary infection; Milk neutrophils; Saúde da glândula mamária; Sensibilidade; Sensitivity; Udder health status. |
Thesagro: |
Bacteriose; Oxigênio; Vaca Leiteira. |
Thesaurus Nal: |
Cattle; Dairy cows; Immune response; Mastitis; Oxygen production; Phagocytosis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178768/1/Intracellular-reactive-oxygen-species.pdf
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Marc: |
LEADER 03573naa a2200397 a 4500 001 2092602 005 2018-06-19 008 2018 bl uuuu u00u1 u #d 100 1 $aMOLINARI, P. C. C. 245 $aIntracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à mastite. 520 $aThe immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity values, which was also confirmed by the Youden index values being far from one. In conclusion, the assessment of milk bovine neutrophil functions could improve our understanding of the cellular basis of mastitis. Although, the intracellular ROS production and S. aureus phagocytosis by milk neutrophil did not have high predictive values to detect intramammary infections, our results strengthen the idea that that poor bovine mammary gland neutrophil phagocytic ability may be associated with high SCC, and might be considered to identify susceptible dairy cows to mastitis. 650 $aCattle 650 $aDairy cows 650 $aImmune response 650 $aMastitis 650 $aOxygen production 650 $aPhagocytosis 650 $aBacteriose 650 $aOxigênio 650 $aVaca Leiteira 653 $aEspecificidade 653 $aInfecção intramamária 653 $aIntracellular reactive 653 $aIntramammary infection 653 $aMilk neutrophils 653 $aSaúde da glândula mamária 653 $aSensibilidade 653 $aSensitivity 653 $aUdder health status 700 1 $aBLAGITZ, M. G. 700 1 $aDELLA LIBERA, A. M. M. P. 700 1 $aBATISTA, C. F. 700 1 $aSOUZA, F. N. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 659-664, abril 2018
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Registros recuperados : 7 | |
1. | | MOLINARI, P. C. C.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; BATISTA, C. F.; SOUZA, F. N. Intracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 659-664, abril 2018 Título em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à...Biblioteca(s): Embrapa Unidades Centrais. |
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2. | | TAKANO, P. V.; BLAGITZ, M. G.; MIRA, C. S.; BATISTA, C. F.; DELLA LIBERA, A. M. M.; SOUZA, F. N. Estudo comparativo das diferentes técnicas empregadas na contagem diferencial de leucócitos no leite. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 773-778, abril 2018 Título em inglês: Comparative study of distinct techniques to determine differential leukocyte counts in milk.
.Biblioteca(s): Embrapa Unidades Centrais. |
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3. | | MARTINS, E. R. M.; BERTAGNON, H. G.; BATISTA, C. F.; GOMES, R. C.; SANTOS, K. R.; BELLINAZZI, J. B.; DELLA LIBERA, A. M. M. P. Influência da suplementação de vitaminas A, D e E na função imune de bezerros alimentados com dieta à base de feno capim tifton (Cynodon ssp.). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 453-459, maio. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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4. | | BLAGITZ, M. G.; SOUZA, F. N.; BATISTA, C. F.; SANTOS, BRUNA P.; PARRA, A. C.; AZEVEDO, L. F. F.; DELLA LIBERA, A. M. M. P. Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis. Pesquisa Veterinária Brasileira, Rio de Janeiro, n. 35, n.1, p.1-5, jan. 2015.Biblioteca(s): Embrapa Unidades Centrais. |
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5. | | BERTAGNON, H. G.; BATISTA, C. F.; BELLINAZZI, J. B.; CONEGLIAN, M. M.; MENDES, A. F.; DELLA LIBERA, A. M. M. P. Identificação de dor após orquiectomia em garrotes: desenvolvimento de escala análogo visual em comparação a parâmetros fisiológicos, padrões comportamentais e expressão facial. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 3, p. 436-443, março 2018 Título em inglês: Pain identification after orchiectomy in young bulls: development of the visual analogue scale compared with physiological parameters, behavioral patterns and facial expression.Biblioteca(s): Embrapa Unidades Centrais. |
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6. | | VIANNA, R. S.; BATISTA, C. F.; LIMA, D. M.; SANTOS, K. R.; GOMES, R. C.; BERTAGNON, H. G.; DELLA LIBERA, A. M. M. P. Perfil citológico pulmonar de bezerros sadios nos três primeiros meses de vida. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 447-452, maio. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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7. | | SOUZA, F. N.; CUNHA, A. F.; ROSA, D. L. S. O.; BRITO, M. A. V. P. e; GUIMARAES, A. S.; MENDONCA, L. C.; SOUZA, G. N.; LAGE, A. P.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Somatic cell count and mastitis pathogen detection in composite and single or duplicate quarter milk samples. Pesquisa Veterinária Brasileira, Brasília, DF. V. 36, N. 9, p. 811-818, set. 2016.Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 7 | |
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