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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
23/10/2018 |
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Data da última atualização: |
23/10/2018 |
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Autoria: |
RAVAGNANI, G. M.; TORRES, M. A.; LEAL, D. F.; MARTINS, S. M. M. K.; PAPA, F. O.; DELL'AQUA JUNIOR, J. A.; ALVARENGA, M. A.; ANDRADE, A. F. C. |
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Afiliação: |
Gisele M. Ravagnani, Núcleo de Pesquisa em Suínos/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Mariana A. Torres, Núcleo de Pesquisa em Suínos/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Diego F. Leal, Núcleo de Pesquisa em Suínos/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Simone M. M. K. Martins, Núcleo de Pesquisa em Suínos/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Frederico O. Papa, Departamento de Reprodução Animal e Radiologia Veterinária/Faculdade de Medicina Veterinária e Zootecnia/Universidade Estadual Paulista - Unesp; José A. Dell?Aqua Junior, Departamento de Reprodução Animal e Radiologia Veterinária/Faculdade de Medicina Veterinária e Zootecnia/Universidade Estadual Paulista - Unesp; Marco A. Alvarenga, Departamento de Reprodução Animal e Radiologia Veterinária/Faculdade de Medicina Veterinária e Zootecnia/Universidade Estadual Paulista - Unesp; André F. C. Andrade, Núcleo de Pesquisa em Suínos/Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP. |
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Título: |
Cryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 9, p. 1726-1730, setembro 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: A manutenção da viabilidade do sêmen suíno criopreservado em palhetas de 0,5mL em baixas concentrações espermáticas é melhor do que em altas concentrações. |
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Conteúdo: |
To date, no studies have been performed evaluating the effect of boar spermatozoa concentration in 0.5mL freezing straws, leading us to examine this question. Each sperm‑rich fraction of the ejaculate (n=25) was diluted at five different sperm concentrations (100, 200, 300, 600 and 800 x 106 spermatozoa/mL), packaged in 0.5mL straws, and subsequently frozen. After thawing, the sperm from all of treatment groups were analyzed to determine motility characteristics using a sperm class analyzer (SCA-CASA), and their plasma and acrosomal membrane integrity, mitochondrial membrane potential, sperm membrane lipid peroxidation and fluidity were analyzed by flow cytometry. An increase in spermatozoa concentration above 300x106 spermatozoa/mL in a 0.5mL straw impaired (p<0.05) the total and progressive motility, curvilinear velocity, straight-line velocity, linearity and beat cross frequency. However, the plasma and acrosomal membrane integrity, mitochondrial membrane potential, membrane lipid peroxidation and fluidity were not influenced (p>0.05) by high spermatozoa concentrations at freezing. Therefore, to increase spermatozoa survival and total and progressive motility after thawing, boar spermatozoa should be frozen at concentrations up to 300x106 spermatozoa/mL. |
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Palavras-Chave: |
Boar semen; Concentração espermática; Criocapacitação; Crioinjúria; Criopreservão; Cryoinjury; Sêmen suíno; Sperm viability. |
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Thesagro: |
Suíno. |
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Thesaurus Nal: |
Cryopreservation; Sperm concentration; Swine. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184875/1/Cryopreservation-of-boar-semen-in-0.5mL-straws.pdf
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Marc: |
LEADER 02572naa a2200361 a 4500
001 2097947
005 2018-10-23
008 2018 bl uuuu u00u1 u #d
100 1 $aRAVAGNANI, G. M.
245 $aCryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability.$h[electronic resource]
260 $c2018
500 $aTítulo em português: A manutenção da viabilidade do sêmen suíno criopreservado em palhetas de 0,5mL em baixas concentrações espermáticas é melhor do que em altas concentrações.
520 $aTo date, no studies have been performed evaluating the effect of boar spermatozoa concentration in 0.5mL freezing straws, leading us to examine this question. Each sperm‑rich fraction of the ejaculate (n=25) was diluted at five different sperm concentrations (100, 200, 300, 600 and 800 x 106 spermatozoa/mL), packaged in 0.5mL straws, and subsequently frozen. After thawing, the sperm from all of treatment groups were analyzed to determine motility characteristics using a sperm class analyzer (SCA-CASA), and their plasma and acrosomal membrane integrity, mitochondrial membrane potential, sperm membrane lipid peroxidation and fluidity were analyzed by flow cytometry. An increase in spermatozoa concentration above 300x106 spermatozoa/mL in a 0.5mL straw impaired (p<0.05) the total and progressive motility, curvilinear velocity, straight-line velocity, linearity and beat cross frequency. However, the plasma and acrosomal membrane integrity, mitochondrial membrane potential, membrane lipid peroxidation and fluidity were not influenced (p>0.05) by high spermatozoa concentrations at freezing. Therefore, to increase spermatozoa survival and total and progressive motility after thawing, boar spermatozoa should be frozen at concentrations up to 300x106 spermatozoa/mL.
650 $aCryopreservation
650 $aSperm concentration
650 $aSwine
650 $aSuíno
653 $aBoar semen
653 $aConcentração espermática
653 $aCriocapacitação
653 $aCrioinjúria
653 $aCriopreservão
653 $aCryoinjury
653 $aSêmen suíno
653 $aSperm viability
700 1 $aTORRES, M. A.
700 1 $aLEAL, D. F.
700 1 $aMARTINS, S. M. M. K.
700 1 $aPAPA, F. O.
700 1 $aDELL'AQUA JUNIOR, J. A.
700 1 $aALVARENGA, M. A.
700 1 $aANDRADE, A. F. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 9, p. 1726-1730, setembro 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/12/2023 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
LEONARDO-SILVA, L.; SILVA, L. B. da; FERREIRA, E. P. de B.; REIS, K. F. D. N. S. dos; NAVES, P. L. F.; MARTIN-DIDONET, C. C. G. |
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Afiliação: |
LUCAS LEONARDO-SILVA, UEG; LARISSA B. DA SILVA, UEG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF; KARINA F. D. N. S. DOS REIS, UEG; PLINIO L. F. NAVES, UEG; CLAUDIA C. G. MARTIN-DIDONET, UEG. |
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Título: |
Assessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Caatinga, v. 36, n. 4, p. 775-784, out./dez. 2023. |
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ISSN: |
1983-2125 |
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Idioma: |
Português |
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Conteúdo: |
Rice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops. MenosRice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies... Mostrar Tudo |
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Palavras-Chave: |
Extracellular enzymes; OCR 16S-23S; RDNA. |
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Thesagro: |
Arroz; Bactéria; Enzima; Fator de Crescimento; Oryza Sativa. |
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Thesaurus NAL: |
Genotype; Plant growth-promoting rhizobacteria; Rice. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159886/1/rev-caatinga-2023.pdf
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Marc: |
LEADER 02594naa a2200325 a 4500
001 2159886
005 2023-12-18
008 2023 bl uuuu u00u1 u #d
022 $a1983-2125
100 1 $aLEONARDO-SILVA, L.
245 $aAssessment of morphophysiological and genotypic diversity of endophytic bacteria isolated from rice (Oryza sativa L.) plants.$h[electronic resource]
260 $c2023
520 $aRice production in Brazil incurs high costs due to the significant use of agrochemicals. Some plant growth-promoting rhizobacteria (PGPR) can be used as alternative to fertilizers and phytosanitary products. Thus, the objective of this study was to characterize endophytic bacteria isolated from roots of rice plants. The isolates were characterized based on colony morphology, antibiotic resistance, carbon sources utilization, enzyme activity (catalase, amylase, protease, cellulase, and lipase), inorganic phosphate solubilization, and the 16S-23S rDNA intergenic region. Morphologically, 68% of the isolates presented a rapid growth rate, 46% presented abundant mucus production, and 77% formed viscous colonies. All isolates were resistant to nalidixic acid and 16% presented resistance to streptomycin. The majority (90%) used monosaccharides and disaccharides in carbon source assays. Most of the isolates (95%) were positive for catalase and 63.6% were positive for amylase, protease, lipase, and cellulase activities. Additionally, 59% of them were able to solubilize phosphate. The mean enzymatic index for amylase, cellulase, and protease was 2.8, 3.5, and 1.7 respectively. The similarity analysis revealed high diversity among the isolates, with similarity indices of 70% (based on morphological characteristics) and 60% (based on the intergenic region 16S-23S rDNA). Considering morphophysiological and genotypic characteristics, three promising isolates should be evaluated in studies under field conditions for the potential development of bioproducts to replace industrially manufactured inputs in rice crops.
650 $aGenotype
650 $aPlant growth-promoting rhizobacteria
650 $aRice
650 $aArroz
650 $aBactéria
650 $aEnzima
650 $aFator de Crescimento
650 $aOryza Sativa
653 $aExtracellular enzymes
653 $aOCR 16S-23S
653 $aRDNA
700 1 $aSILVA, L. B. da
700 1 $aFERREIRA, E. P. de B.
700 1 $aREIS, K. F. D. N. S. dos
700 1 $aNAVES, P. L. F.
700 1 $aMARTIN-DIDONET, C. C. G.
773 $tRevista Caatinga$gv. 36, n. 4, p. 775-784, out./dez. 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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