Data/hora: 23/09/2024 23:58:23

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	06/10/2021	
Data da última atualização: 	10/06/2022	
Autoria: 	IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I.	
Afiliação: 	NATASHA SANT´ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JØRGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP.	
Título: 	Development of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Applied Microbiology and Biotechnology, v. 105, n. 20, p. 7913-7933, 2021.	
ISSN: 	1432-0614	
DOI: 	https://doi.org/10.1007/s00253-021-11576-5	
Idioma: 	Inglês	
Conteúdo: 	Abstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5&#8201;×&#8201;107 blastospores mL&#8722;1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage.	
Palavras-Chave: 	Convective drying.	
Thesagro: 	Cigarrinha; Fermentação; Formulação; Fungo Entomógeno; Fungo Para Controle Biológico; Pó Molhável; Praga de Planta.	
Thesaurus Nal: 	Biological control agents; Dalbulus maidis; Entomopathogenic fungi; Liquid state fermentation; Metarhizium robertsii; Pesticide formulations; Wettable powders.	
Categoria do assunto: 	H Saúde e Patologia	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (17036-1) - UPC - AP - DD -  -  -  - DIS