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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/03/2022 |
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Data da última atualização: |
24/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELABONA, P. S.; CODIMA, C. A.; RAMONI, J.; ZUBIETA, M. P.; ARAÚJO, B. M. de; FARINAS, C. S.; PRADELLA, J. G. C.; SEIBOTH, B. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
The impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Bioresource Technology, v. 313, 12361, 2020. |
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Páginas: |
1 - 7 |
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ISSN: |
0960-8524 |
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DOI: |
https://doi.org/10.1016/j.biortech.2020.123616 |
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Idioma: |
Inglês |
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Conteúdo: |
Trichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1(20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum. |
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Palavras-Chave: |
Glycoside hydrolases; LAE1; Overexpression. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01931naa a2200277 a 4500
001 2141187
005 2022-06-24
008 2022 bl uuuu u00u1 u #d
022 $a0960-8524
024 7 $ahttps://doi.org/10.1016/j.biortech.2020.123616$2DOI
100 1 $aDELABONA, P. S.
245 $aThe impact of putative methyltransferase overexpression on the Trichoderma harzianum cellulolytic system for biomass conversion.$h[electronic resource]
260 $c2022
300 $a1 - 7
520 $aTrichoderma harzianum has attracting attention for its potential alternative use in biofuel production, due to a recognized competence for high diversity glycoside hydrolases (GH) enzyme complex, including higher β-glucosidases and auxiliary proteins, using low-cost carbon sources. This strain constitutively overexpressed the global regulator putative methyltransferase - LAE1, in order to improve the GHs production. The recombinant strain achieved 79-fold increase in lae1 expression and high GHs productivity. The evaluation of the LAE1 impact to induce the GHs used soluble and lignocellulose inexpensive carbon sources in a stirred-tank bioreactor. Using sugarcane bagasse with sucrose, the overexpression of lae1 resulted in significantly increment of gh61b (31x), cel7a (25x), bgl1(20x) and xyn3 (20x) genes expression. Reducing sugar released from pretreated sugarcane bagasse, which hydrolyzed by recombinant crude enzyme cocktail, achieved 41% improvement. Therefore, lae1 overexpression effectively is a promising improving GHs target for biomass degradation by T. harzianum.
653 $aGlycoside hydrolases
653 $aLAE1
653 $aOverexpression
700 1 $aCODIMA, C. A.
700 1 $aRAMONI, J.
700 1 $aZUBIETA, M. P.
700 1 $aARAÚJO, B. M. de
700 1 $aFARINAS, C. S.
700 1 $aPRADELLA, J. G. C.
700 1 $aSEIBOTH, B.
773 $tBioresource Technology$gv. 313, 12361, 2020.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
14/08/2012 |
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Data da última atualização: |
15/08/2017 |
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Autoria: |
CURI, R. A.; KRAUSKOPF, M. K.; HADLICH, J. C.; FORTES, M. R. S.; VANKAN, D. M.; SILVA, J. A. V.; OLIVEIRA, H. N. de; MOTA, M. D. S. da. |
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Afiliação: |
Rogério Abdallah Curi, Universidade Estadual Paulista/Faculdade de Medicina Veterinária e Zootecnia; Monique Marcondes Krauskopf, Universidade Estadual Paulista/Faculdade de Medicina Veterinária e Zootecnia; Janaína Conte Hadlich, Universidade Estadual Paulista/Faculdade de Medicina Veterinária e Zootecnia; Marina Rufino Salinas Fortes, The University of Queensland, School of Veterinary Science; Dianne Margaret Vankan, The University of Queensland, School of Veterinary Science; Josineudson Augusto II Vasconcelos Silva, Universidade Estadual Paulista/Faculdade de Medicina Veterinária e Zootecnia; Henrique Nunes de Oliveira, Unesp/Faculdade de Ciências Agrárias e Veterinárias; Marcílio Dias Silveira da Mota, Universidade Estadual Paulista/Faculdade de Medicina Veterinária e Zootecnia. |
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Título: |
Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 47, n. 2, p. 294-302, fev. 2012. |
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Idioma: |
Português |
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Notas: |
Título em português: SNPs candidatos para características da carcaça e da carne em Nelore e nos seus cruzamentos com Bos taurus. |
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Conteúdo: |
The objective of this work was to evaluate the effects of single?nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non?informative in Nelore animals. In crossbred animals, the IGF1 C allele was associated with greater REA. However, this relation was not significant after Bonferroni correction for multiple testing. The A allele of the MSTN polymorphism was absent in Nelore cattle and was only found in two crossbred animals. The polymorphisms of MYOD1 and MYF5 were little informative in Nelore animals with G allele frequency of 0.097 and A allele frequency of 0.031, respectively. These markers show no association with the analyzed traits in the total sample of evaluated animals. |
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Palavras-Chave: |
Gene candidato; Maciez da carne. |
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Thesagro: |
Bos Taurus; Carcaça; Gado nelore. |
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Thesaurus NAL: |
Meat quality; Zebu. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/63759/1/Candidate-SNPs....pdf
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Marc: |
LEADER 02254naa a2200301 a 4500
001 1931238
005 2017-08-15
008 2012 bl uuuu u00u1 u #d
100 1 $aCURI, R. A.
245 $aCandidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus.
260 $c2012
500 $aTítulo em português: SNPs candidatos para características da carcaça e da carne em Nelore e nos seus cruzamentos com Bos taurus.
520 $aThe objective of this work was to evaluate the effects of single?nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non?informative in Nelore animals. In crossbred animals, the IGF1 C allele was associated with greater REA. However, this relation was not significant after Bonferroni correction for multiple testing. The A allele of the MSTN polymorphism was absent in Nelore cattle and was only found in two crossbred animals. The polymorphisms of MYOD1 and MYF5 were little informative in Nelore animals with G allele frequency of 0.097 and A allele frequency of 0.031, respectively. These markers show no association with the analyzed traits in the total sample of evaluated animals.
650 $aMeat quality
650 $aZebu
650 $aBos Taurus
650 $aCarcaça
650 $aGado nelore
653 $aGene candidato
653 $aMaciez da carne
700 1 $aKRAUSKOPF, M. K.
700 1 $aHADLICH, J. C.
700 1 $aFORTES, M. R. S.
700 1 $aVANKAN, D. M.
700 1 $aSILVA, J. A. V.
700 1 $aOLIVEIRA, H. N. de
700 1 $aMOTA, M. D. S. da
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 47, n. 2, p. 294-302, fev. 2012.
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Embrapa Unidades Centrais (AI-SEDE) |
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