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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/12/2023 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRUZ, T. I. da; ROCHA, D. C.; LANNA, A. C.; DEDICOVA, B.; VIANELLO, R. P.; BRONDANI, C. |
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Afiliação: |
THAÍS IGNEZ DA CRUZ, UFG; DHIÔVANNA CORRÊIA ROCHA, IAC; ANNA CRISTINA LANNA, CNPAF; BEATA DEDICOVA, SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES; ROSANA PEREIRA VIANELLO, CNPAF; CLAUDIO BRONDANI, CNPAF. |
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Título: |
Calcium-dependent protein kinase 5 (OsCPK5) overexpression in upland rice (Oryza sativa L.) under water deficit. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plants, v. 12, n. 22, 3826, Nov. 2023. |
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ISSN: |
2223-7747 |
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DOI: |
https://doi.org/10.3390/plants12223826 |
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Idioma: |
Inglês |
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Conteúdo: |
Water deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plants under water deficit exhibited more leaf senescence, while the OsCPK5-overexpressing plants retained their green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, indicating the potential for future development of more drought-tolerant rice cultivars. MenosWater deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plant... Mostrar Tudo |
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Thesagro: |
Arroz; Deficiência Hídrica; Oryza Sativa; Resistência a Seca. |
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Thesaurus Nal: |
Drought; Gene expression; Rice; Senescence (aging); Water use efficiency. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159651/1/plants-2023.pdf
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Marc: |
LEADER 02692naa a2200313 a 4500
001 2159651
005 2023-12-18
008 2023 bl uuuu u00u1 u #d
022 $a2223-7747
024 7 $ahttps://doi.org/10.3390/plants12223826$2DOI
100 1 $aCRUZ, T. I. da
245 $aCalcium-dependent protein kinase 5 (OsCPK5) overexpression in upland rice (Oryza sativa L.) under water deficit.$h[electronic resource]
260 $c2023
520 $aWater deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plants under water deficit exhibited more leaf senescence, while the OsCPK5-overexpressing plants retained their green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, indicating the potential for future development of more drought-tolerant rice cultivars.
650 $aDrought
650 $aGene expression
650 $aRice
650 $aSenescence (aging)
650 $aWater use efficiency
650 $aArroz
650 $aDeficiência Hídrica
650 $aOryza Sativa
650 $aResistência a Seca
700 1 $aROCHA, D. C.
700 1 $aLANNA, A. C.
700 1 $aDEDICOVA, B.
700 1 $aVIANELLO, R. P.
700 1 $aBRONDANI, C.
773 $tPlants$gv. 12, n. 22, 3826, Nov. 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Arroz e Feijão; Embrapa Solos. |
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Data corrente: |
30/01/2006 |
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Data da última atualização: |
14/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
MADARI, B. E.; REEVES, J. B.; COELHO, M. R.; MACHADO, P. L. O. A.; DE-POLLI, H.; COELHO, R. M.; BENITES, V. M.; SOUZA, L. F.; McCARTY, G. W. |
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Afiliação: |
BEATA EMOKE MADARI, CNPAF; JAMES B. REEVES, USDA; MAURICIO RIZZATO COELHO, CNPS; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPAF; HELVECIO DE POLLI, CNPAB; RICARDO M. COELHO, IAC; VINICIUS DE MELO BENITES, CNPS; LUCAS F. SOUZA; GREGORY W. MCCARTY, USDA. |
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Título: |
Mid- and near-infrared spectroscopic determination of carbon in a diverse set of soils from the Brazilian national soil collection. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Spectroscopy Letters, v. 38, n. 6, p. 721-740, 2005. |
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DOI: |
https://doi.org/10.1080/00387010500315876 |
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Idioma: |
Inglês |
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Conteúdo: |
Calibrations for soil carbon content measured by combustion (total carbon, TC) and chromate oxidation by a modified Walkley-Black method (Walkley-Black carbon, WBC) from the Brazilian National Soil Collection were made using Fourier-transform near (1100 to 2500 nm; NIRS) and mid-infrared diffuse reflectance (2,500 to 25,000 nm; DRIFTS) spectroscopy combined with partial least squares (PLS). Calibration sets of sample populations of different carbon ranges, soil taxonomic classes, and soil textural groups were established. These are for TC ranges between 0.4 to 555.0, 0.4 to 99.1, and 0.4 to 39.9 g kg21: for WBC 0.2 to 401.0, 0.2 to 66.0, and 0.2 to 66.0, and 0.2 to 30.0 g kg21: for soil taxonomic classes Ferralsols and Acrisols; and for soil textural groups very clayey, clayey, and medium textures were examined. Calibrations obtained for the largest TC and WBC ranges were better compared to the lower ones, but lower root mean squared deviation (RMSD) and relative difference (RD ¼ RMSD/mean value) were found for the lower carbon ranges. Taxonomic soil class was not an adequate criterium for calibration set formation. Soil texture had effect on calibrations, especially using NIR, because of the particle size effect to which NIR was more sensitive than mid-IR. In general, DRIFTS showed better performance than NIRS. NIRS only outperformed DRIFTS when used with calibration set fairly homogeneous in its particle size distribution. Results demonstrated that while calibrations can be developed using either DRIFTS or NIRS for even a very diverse set of soil samples, which will determine C over a wide range of concentrations inherent in such a diverse set, it is desirable to seperate sample populations by soil textural properties and choose the adequate spectral range (NIR or mid-IR) based on the textural group, for calibration development to achieve more accurate results. MenosCalibrations for soil carbon content measured by combustion (total carbon, TC) and chromate oxidation by a modified Walkley-Black method (Walkley-Black carbon, WBC) from the Brazilian National Soil Collection were made using Fourier-transform near (1100 to 2500 nm; NIRS) and mid-infrared diffuse reflectance (2,500 to 25,000 nm; DRIFTS) spectroscopy combined with partial least squares (PLS). Calibration sets of sample populations of different carbon ranges, soil taxonomic classes, and soil textural groups were established. These are for TC ranges between 0.4 to 555.0, 0.4 to 99.1, and 0.4 to 39.9 g kg21: for WBC 0.2 to 401.0, 0.2 to 66.0, and 0.2 to 66.0, and 0.2 to 30.0 g kg21: for soil taxonomic classes Ferralsols and Acrisols; and for soil textural groups very clayey, clayey, and medium textures were examined. Calibrations obtained for the largest TC and WBC ranges were better compared to the lower ones, but lower root mean squared deviation (RMSD) and relative difference (RD ¼ RMSD/mean value) were found for the lower carbon ranges. Taxonomic soil class was not an adequate criterium for calibration set formation. Soil texture had effect on calibrations, especially using NIR, because of the particle size effect to which NIR was more sensitive than mid-IR. In general, DRIFTS showed better performance than NIRS. NIRS only outperformed DRIFTS when used with calibration set fairly homogeneous in its particle size distribution. Results demonstrated that while calibrations can b... Mostrar Tudo |
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Palavras-Chave: |
DRIFTS; Mid-infrared; Near-infrared; NIRS; PLS. |
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Thesagro: |
Carbono; Solo. |
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Thesaurus NAL: |
carbon; chemometrics; soil. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02877naa a2200349 a 4500
001 1213859
005 2022-05-14
008 2005 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/00387010500315876$2DOI
100 1 $aMADARI, B. E.
245 $aMid- and near-infrared spectroscopic determination of carbon in a diverse set of soils from the Brazilian national soil collection.$h[electronic resource]
260 $c2005
520 $aCalibrations for soil carbon content measured by combustion (total carbon, TC) and chromate oxidation by a modified Walkley-Black method (Walkley-Black carbon, WBC) from the Brazilian National Soil Collection were made using Fourier-transform near (1100 to 2500 nm; NIRS) and mid-infrared diffuse reflectance (2,500 to 25,000 nm; DRIFTS) spectroscopy combined with partial least squares (PLS). Calibration sets of sample populations of different carbon ranges, soil taxonomic classes, and soil textural groups were established. These are for TC ranges between 0.4 to 555.0, 0.4 to 99.1, and 0.4 to 39.9 g kg21: for WBC 0.2 to 401.0, 0.2 to 66.0, and 0.2 to 66.0, and 0.2 to 30.0 g kg21: for soil taxonomic classes Ferralsols and Acrisols; and for soil textural groups very clayey, clayey, and medium textures were examined. Calibrations obtained for the largest TC and WBC ranges were better compared to the lower ones, but lower root mean squared deviation (RMSD) and relative difference (RD ¼ RMSD/mean value) were found for the lower carbon ranges. Taxonomic soil class was not an adequate criterium for calibration set formation. Soil texture had effect on calibrations, especially using NIR, because of the particle size effect to which NIR was more sensitive than mid-IR. In general, DRIFTS showed better performance than NIRS. NIRS only outperformed DRIFTS when used with calibration set fairly homogeneous in its particle size distribution. Results demonstrated that while calibrations can be developed using either DRIFTS or NIRS for even a very diverse set of soil samples, which will determine C over a wide range of concentrations inherent in such a diverse set, it is desirable to seperate sample populations by soil textural properties and choose the adequate spectral range (NIR or mid-IR) based on the textural group, for calibration development to achieve more accurate results.
650 $acarbon
650 $achemometrics
650 $asoil
650 $aCarbono
650 $aSolo
653 $aDRIFTS
653 $aMid-infrared
653 $aNear-infrared
653 $aNIRS
653 $aPLS
700 1 $aREEVES, J. B.
700 1 $aCOELHO, M. R.
700 1 $aMACHADO, P. L. O. A.
700 1 $aDE-POLLI, H.
700 1 $aCOELHO, R. M.
700 1 $aBENITES, V. M.
700 1 $aSOUZA, L. F.
700 1 $aMcCARTY, G. W.
773 $tSpectroscopy Letters$gv. 38, n. 6, p. 721-740, 2005.
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