Data/hora: 06/06/2024 04:44:23

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 30/09/2008
Data da última atualização: | 03/02/2009
Autoria: | PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T.
Afiliação: | Ana Flávia Parente, UnB; Ildinete Silva-Pereira, UnB; José Ivo Baldani, Embrapa Agrobiologia; Victor Hugo da Silva Tibúrcio, UnB; Sônia Nair Báo, UnB; Marlene T. de Souza, UnB.
Título: | Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions.
Ano de publicação: | 2008
Fonte/Imprenta: | Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008.
Idioma: | Inglês
Notas: | Parceria: UnB.
Conteúdo: | Collectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis  host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein.
Palavras-Chave: | Cana-de-açúcar.
Thesagro: | Bacillus Thuringiensis; Controle Biológico; Diatraea Saccharalis.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (35376-1) - UPC - SP - -- - SP 2008-24237 -  - 0024237 - DIS