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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, G. A. F.; DANTAS, J. L. L.; OLIVEIRA, E. J. de. |
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Afiliação: |
G. A. F. OLIVEIRA, UFRB; JORGE LUIZ LOYOLA DANTAS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Development and validation of minisatellite markers for Carica papaya. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Biologia Plantarum, v. 59, n.4, p 686-694, 2015. |
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DOI: |
10.1007/s10535-015-0551-9686 |
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Idioma: |
Inglês |
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Conteúdo: |
Whereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding. MenosWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. ... Mostrar Tudo |
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Thesagro: |
Carica Papaya; Mamão; Variação genética. |
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Thesaurus Nal: |
Genetic variation. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02343naa a2200205 a 4500
001 2028333
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s10535-015-0551-9686$2DOI
100 1 $aOLIVEIRA, G. A. F.
245 $aDevelopment and validation of minisatellite markers for Carica papaya.$h[electronic resource]
260 $c2015
520 $aWhereas microsatellite markers are well described, there are few studies investigating minisatellites. Therefore, this study aimed to identify, characterize, and validate minisatellite loci for papaya (Carica papaya L.). The entire papaya genome, which covers 330 Mb, was used to mine minisatellites with motifs ranging from 6 to 500 bp, and at least six replicates and 82 loci were validated in a set of 24 accessions. A total of 1 730 minisatellite loci were identified, located in 695 sequences, with an average of one minisatellite every 156 kb. Variation in GC content ranged from 0.00 to 83.84 % with an average of 28.84 % indicating that these papaya minisatellites are AT rich. Motifs of up to 20 bases represented 71.45 % of the markers. In addition, the observed variation in the number of motif repeats was from 6 to 186 with an average of 9.27 per minisatellite. Independent of the classification, the frequency of minisatellites decreased with an increase in the number of repeating units. Among the validated loci, 48.78 % were found to be polymorphic, and the number of alleles (NA) ranged from two to seven with a mean of 3.10. The average polymorphic information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) were 0.38, 0.43, and 0.11, respectively. According to genetic diversity parameters, such as NA, He, and PIC, no significant correlations were found among the size of motifs, the number of repeats, and the GC content of these minisatellites. This study clearly demonstrates the polymorphism of minisatellites and their potential use in detection of intraspecific genetic variations in C. papaya. The results will be useful when managing genetic resources and plant breeding.
650 $aGenetic variation
650 $aCarica Papaya
650 $aMamão
650 $aVariação genética
700 1 $aDANTAS, J. L. L.
700 1 $aOLIVEIRA, E. J. de
773 $tBiologia Plantarum$gv. 59, n.4, p 686-694, 2015.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 175 | |
| 85. |  | DANTAS, J. L. L.; SOUZA, A. da S.; JUNGHANS, D. T.; LIMA, J. F. de. Variedades e características da planta. In: DANTAS, J. L. L.; JUNGHANS, D. T.; LIMA, J. F. de (Ed.). Mamão: o produtor pergunta, a Embrapa responde. 2. ed. rev. e atual. Brasília, DF: Embrapa, 2013. 170 p. il. (Coleção 500 perguntas, 500 respostas). p. 15-28. Cap. 1.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 91. | | BUENO, A.; FUKUDA, W. M. G.; CARDOSO, E. M. R.; DANTAS, J. L. L. Seleção de genótipos de mandioca no trópico úmido brasileiro. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Resumos. Belém, PA: EMBRAPA-CPATU, 1984. p. 172-173. (EMBRAPA-CPATU. Documentos, 31).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 92. | | BUENO, A.; FUKUDA, W. M. G.; CARDOSO, E. M. R.; DANTAS, J. L. L. Seleção de genótipos de mandioca no trópico úmido. In: SIMPÓSIO DO TRÓPICO ÚMIDO, 1., 1984, Belém, PA. Anais. Belém, PA: EMBRAPA-CPATU, 1986. v. 3, p. 97-105. (EMBRAPA-CPATU. Documentos, 36). v.3 Culturas temporarias.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 95. | | CASTELLEN, M. S.; DANTAS, J. L. L.; GOEDERT, C. O.; FERREIRA, F. R. Sub-rede de coleta, caracterização e conservação ex situ de germoplasma de fruteiras. PC 4 - RENARGEN. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 5., 2005, Montevideo, Uruguay. Resúmenes... Montevideo: Instituto Nacional de Investigación Agropecuaria: Universidad de la República, Facultad de Agronomía, 2005. p. 27.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 98. | | COSTA, T. P. P.; LIMA NETO, F. P.; OLIVEIRA, E. J.; DANTAS, J. L. L.; SANTOS, R. O. Avaliação preliminar do potencial germinativo de linhagens e híbridos de mamoeiro (Carica papaya l.) em telado. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 21., 2010, Natal. Frutas: saúde, inovação e responsabilidade: anais. Natal: SBF, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 99. | | CARDOSO, M. G. S.; SOUZA, A. da S.; VIDAL, A. M.; DANTAS, J. L. L.; MORAIS-LINO, L. S. Avaliação dos níveis de BAP na multiplicação in vitro do mamoeiro. Revista Brasileira de Horticultura Ornamental, Campinas, v. 13, 1226-1229, 2007. Suplemento. Edição dos Resumos do XVI Congresso Brasileiro de Floricultura e Plantas Ornamentais; III Congresso Brasileiro de Cultura de Tecidos e Plantas; I Simpósio de Plantas Ornamentais Nativas, Goiânia, set. 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 100. | | REIS, R. C.; VIANA, E. de S.; JESUS, J. L. de; DANTAS, J. L. L.; LUCENA, R. S. Caracterização físico-química de frutos de novos híbridos e linhagens de mamoeiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 50, n. 3, p. 210-217, mar. 2015. Título em inglês: Physicochemical characterization of new hybrids and inbred lines of papaya.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| Registros recuperados : 175 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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