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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
22/05/2017 |
Data da última atualização: |
22/05/2017 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
RANGEL, L. F. S.; ZANI, A.; CAPUTI, B.; DALMEDICO, G.; MILTENBURG, G.; BARANCELLI, G. V.; MIELE, M.; LIMA, G. J. M. M. de. |
Afiliação: |
LUIZ FERNANDO SARMENTO RANGEL, Sindirações; ARIOVALDO ZANI, CBNA; BRUNO CAPUTI, Sindirações; GEORDANO DALMEDICO, CNPSA; GODOFREDO MILTENBURG, CBNA; GIOVANA V. BARANCELLI, ESALQ; MARCELO MIELE, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
Título: |
Destinação de animais mortos. O que pensa a indústria de alimentação animal? Cadáveres devem ou não entrar na cadeia de alimentação animal? |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: WORKSHOP CBNA SOBRE ALIMENTOS SEGUROS, 2017, Campinas. Anais... Campinas: CBNA, 2017. |
Idioma: |
Português |
Palavras-Chave: |
Carcaça de animais mortos. |
Thesagro: |
Cadeia alimentar; Indústria alimentícia; Nutrição animal; Ração. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160102/1/final8561.pdf
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Marc: |
LEADER 00832nam a2200241 a 4500 001 2069836 005 2017-05-22 008 2017 bl uuuu u00u1 u #d 100 1 $aRANGEL, L. F. S. 245 $aDestinação de animais mortos. O que pensa a indústria de alimentação animal? Cadáveres devem ou não entrar na cadeia de alimentação animal?$h[electronic resource] 260 $aIn: WORKSHOP CBNA SOBRE ALIMENTOS SEGUROS, 2017, Campinas. Anais... Campinas: CBNA$c2017 650 $aCadeia alimentar 650 $aIndústria alimentícia 650 $aNutrição animal 650 $aRação 653 $aCarcaça de animais mortos 700 1 $aZANI, A. 700 1 $aCAPUTI, B. 700 1 $aDALMEDICO, G. 700 1 $aMILTENBURG, G. 700 1 $aBARANCELLI, G. V. 700 1 $aMIELE, M. 700 1 $aLIMA, G. J. M. M. de
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
27/03/2023 |
Data da última atualização: |
12/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ. |
Título: |
Coffee microspore cultivation to attain doubled-haploid plantlets. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. |
DOI: |
10.17660/ActaHortic.2023.1359.14 |
Idioma: |
Inglês |
Notas: |
Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
Conteúdo: |
Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. MenosIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control ant... Mostrar Tudo |
Thesagro: |
Embriogénese; Pólen; Rubiaceae. |
Thesaurus NAL: |
Embryogenesis; Pollen. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02752naa a2200241 a 4500 001 2152776 005 2023-04-12 008 2023 bl uuuu u00u1 u #d 024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI 100 1 $aANGELO, P. C. da S. 245 $aCoffee microspore cultivation to attain doubled-haploid plantlets.$h[electronic resource] 260 $c2023 500 $aApresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. 520 $aIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. 650 $aEmbryogenesis 650 $aPollen 650 $aEmbriogénese 650 $aPólen 650 $aRubiaceae 700 1 $aPEREIRA, L. F. P. 700 1 $aSERA, G. H. 700 1 $aSETA, T. 773 $tActa Horticulturae$gv. 31, n. 1359, p. 123-130, 2023.
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