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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
27/10/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
VOLLÚ, R. E.; MOTA, F. F. da; GOMES, E. A.; SELDIN, L. |
Afiliação: |
Renata Estebanez Vollú, UFRJ; Fabio Faria da Mota, UFRJ; ELIANE APARECIDA GOMES, CNPMS; Lucy Seldin, UFRJ. |
Título: |
Cyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of Paenibacillus graminis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Biotechnology Letters, Dordrecht, v. 30, n. 5, p. 929-935, 2008. |
DOI: |
10.1007/s10529-008-9635-3 |
Idioma: |
Inglês |
Conteúdo: |
Paenibacillus graminis strains were described recently as cyclodextrin (CD) producers. Cyclodextrins are produced by cyclodextrin glucanotransferase (CGTase) which has not been characterized in P. graminis. Similar amounts of ?- and ?-CDs were produced by P. graminis (MC22.13) and P. macerans (LMD24.10T). Primers were designed to sequence the gene encoding CGTase from P. graminis. A phylogenetic tree was constructed and P. graminis CGTase protein showed to be closer (79.4% protein identity) to P. macerans |P31835|. Hybridization studies suggested that the gene encoding CGTase is located in different positions in the genomes of P. macerans and P. graminis. |
Palavras-Chave: |
Cyclodextrin; Cyclodextrin glucanotransferase; Nucleotide sequence; Paenibacillus graminis. |
Thesaurus NAL: |
phylogeny. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01399naa a2200229 a 4500 001 1491405 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1007/s10529-008-9635-3$2DOI 100 1 $aVOLLÚ, R. E. 245 $aCyclodextrin production and genetic characterization of cyclodextrin glucanotranferase of Paenibacillus graminis.$h[electronic resource] 260 $c2008 520 $aPaenibacillus graminis strains were described recently as cyclodextrin (CD) producers. Cyclodextrins are produced by cyclodextrin glucanotransferase (CGTase) which has not been characterized in P. graminis. Similar amounts of ?- and ?-CDs were produced by P. graminis (MC22.13) and P. macerans (LMD24.10T). Primers were designed to sequence the gene encoding CGTase from P. graminis. A phylogenetic tree was constructed and P. graminis CGTase protein showed to be closer (79.4% protein identity) to P. macerans |P31835|. Hybridization studies suggested that the gene encoding CGTase is located in different positions in the genomes of P. macerans and P. graminis. 650 $aphylogeny 653 $aCyclodextrin 653 $aCyclodextrin glucanotransferase 653 $aNucleotide sequence 653 $aPaenibacillus graminis 700 1 $aMOTA, F. F. da 700 1 $aGOMES, E. A. 700 1 $aSELDIN, L. 773 $tBiotechnology Letters, Dordrecht$gv. 30, n. 5, p. 929-935, 2008.
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