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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/03/2012 |
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Data da última atualização: |
22/03/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA JÚNIOR, A. A.; MAGALHÃES, C. G.; SALES, E. B.; D'AMBROS, R. M.; ZANELLA, J. R. C.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P. dos. |
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Afiliação: |
ANTONIO AUGUSTO FONSECA JÚNIOR, LANAGRO; CRISTINA GONÇALVES MAGALHÃES, LANAGRO; ERICA BRAVO SALES, LANAGRO; REGIA MARIA D'AMBROS, CEDISA; JANICE REIS CIACCI ZANELLA, CNPSA; MARCOS BRYAN HEINEMANN, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JENNER KARLISSON PIMENTA DOS REIS, UFMG. |
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Título: |
Genotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
ISRN Microbiology, v. 2011. Doi:10.5402/2011/458294. |
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Idioma: |
Inglês |
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Notas: |
Projeto/Plano de Ação: 03.09.00.046. |
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Conteúdo: |
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. |
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Palavras-Chave: |
PCR. |
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Thesagro: |
Genótipo; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01560naa a2200253 a 4500
001 1919948
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aFONSECA JÚNIOR, A. A.
245 $aGenotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis.$h[electronic resource]
260 $c2011
500 $aProjeto/Plano de Ação: 03.09.00.046.
520 $aSuid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
650 $aGenótipo
650 $aVírus
653 $aPCR
700 1 $aMAGALHÃES, C. G.
700 1 $aSALES, E. B.
700 1 $aD'AMBROS, R. M.
700 1 $aZANELLA, J. R. C.
700 1 $aHEINEMANN, M. B.
700 1 $aLEITE, R. C.
700 1 $aREIS, J. K. P. dos
773 $tISRN Microbiology$gv. 2011. Doi:10.5402/2011/458294.
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| 3. |  | COSTA, C. P. da; FERRAZ, E.; WANDERLEY, L. J. da G.; MELO, P. C. T. de; SOUTO, J. P. de M.; LIMA, D. T. de; QUEIROZ, M. A. de; CANDEIA, J. A.; SILVA, H. M. e. Tomate para industria IPA 1-2. Pesquisa Agropecuaria Pernambucana, Recife, v. 2, n. 1, p. 88-93, 1978.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
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