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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
25/01/2013 |
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Data da última atualização: |
15/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, R. C.; ANDREOTTI, R.; SILVA, E. A. e; ELISÂNGELA PEREIRA; SATO, T.; THOMAZ-SOCCOL, V. |
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Afiliação: |
RODRIGO CASQUERO CUNHA, COLSISTA CNPGC; RENATO ANDREOTTI, CNPGC; ELAINE ARAUJO E SILVA, Center for Zoonosis Control in Campo Grande; ELISÂNGELA PEREIRA, Universidade Federal do Mato Grosso do Sul - UFMS; TAYRA SATO, BOLSISTA CNPGC; VANETE THOMAZ-SOCCOL, Positive University. |
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Título: |
Laboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the center for zoonosis control in Campo Grande - MS, Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Archives of Veterinary Science, v. 17, n. 4, p. 17-26, 2012. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Diagnóstico laboratorial e sinais clínicos para Leishmaniose visceral canina em cães examinados no Centro de Controle de Zoonoses de Campo Grande - MS. |
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Conteúdo: |
Visceral leishmaniasis is atype of zoonosis caused by several Leishmaniaspecies endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical signs of leishmaniasis in dogs in Campo Grande, Mato Grosso do Sul, Brazil. Samples from affected animals were analyzedusing indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays to determine the optimal diagnostic tool for use on animals that present clinical symptoms. A predominance of clinical symptoms affecting the integumentary system was observed, and splenomegaly and hepatomegaly were the most important pathological signs. Among the diagnostic tests, the greatest agreement was seen between ELISA and IFA, followed by ELISA and PCR, and finally IFA and PCR. PCR diagnostic results showed the greatest extent of correlation with clinical signs, followed by ELISA and then IFA. When choosing a diagnostic method, veterinarians should consider the clinical signs and health status of the patient. |
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Palavras-Chave: |
IFA; PCR; RV1/RV2 primers. |
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Thesagro: |
ELISA. |
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Thesaurus Nal: |
visceral leishmaniasis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02120naa a2200253 a 4500
001 1946428
005 2020-04-15
008 2012 bl uuuu u00u1 u #d
100 1 $aCUNHA, R. C.
245 $aLaboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the center for zoonosis control in Campo Grande - MS, Brazil.$h[electronic resource]
260 $c2012
500 $aTítulo em português: Diagnóstico laboratorial e sinais clínicos para Leishmaniose visceral canina em cães examinados no Centro de Controle de Zoonoses de Campo Grande - MS.
520 $aVisceral leishmaniasis is atype of zoonosis caused by several Leishmaniaspecies endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical signs of leishmaniasis in dogs in Campo Grande, Mato Grosso do Sul, Brazil. Samples from affected animals were analyzedusing indirect fluorescent antibody (IFA) tests, an enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) assays to determine the optimal diagnostic tool for use on animals that present clinical symptoms. A predominance of clinical symptoms affecting the integumentary system was observed, and splenomegaly and hepatomegaly were the most important pathological signs. Among the diagnostic tests, the greatest agreement was seen between ELISA and IFA, followed by ELISA and PCR, and finally IFA and PCR. PCR diagnostic results showed the greatest extent of correlation with clinical signs, followed by ELISA and then IFA. When choosing a diagnostic method, veterinarians should consider the clinical signs and health status of the patient.
650 $avisceral leishmaniasis
650 $aELISA
653 $aIFA
653 $aPCR
653 $aRV1/RV2 primers
700 1 $aANDREOTTI, R.
700 1 $aSILVA, E. A. e
700 1 $aELISÂNGELA PEREIRA
700 1 $aSATO, T.
700 1 $aTHOMAZ-SOCCOL, V.
773 $tArchives of Veterinary Science$gv. 17, n. 4, p. 17-26, 2012.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/07/2019 |
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Data da última atualização: |
19/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
AQUINO, A. M. de; CANELLAS, L. P.; SILVA, A. P. S. da; CANELLAS, N. O.; LIMA, L. da S.; OLIVARES, F. L.; PICCOLO, A.; SPACCINI, R. |
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Afiliação: |
ADRIANA MARIA DE AQUINO, CNPAB; UENF; UENF; UENF; UENF; UENF; Università di Napoli Federico; Università di Napoli Federico. |
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Título: |
Evaluation of molecular properties of humic acids from vermicompost by 13 C CPMAS NMR spectroscopy and thermochemolysis GC MS |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Analytical and Applied Pyrolysis, v .141, 104634, August 2019. |
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DOI: |
https://doi.org/10.1016/j.jaap.2019.104634 |
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Idioma: |
Inglês |
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Conteúdo: |
The molecular characteristics of different vermicomposts, produced with variable rates of cattle manure and cotton residues, and of related humic acids were determined by 13C-CPMAS NMR and Off-line pyrolysis THM-GC?MS. The analyses highlighted a composition of mature vermicomposts based on differential amounts of lignocellulosic fraction and alkyl compounds inherited from original biomasses, thus revealing a decrease of overall hydrophobic character at increasing amounts of cotton input. The evaluation of molecular parameters derived from both solid state NMR spectra and from the specific biomarkers released by thermochemolysis suggested that the humification process was mainly characterized by the selective preservation of specific lignin derivatives. The potential bioactive properties of humic acids were hence evaluated in a bioassays test on maize seedlings. The humic extracts showed a differential response in root growth-promoting effect, depending on the specific molecular features. The higher bioactivity found in humic components isolated from vermicompost with no or low addition of cotton residues, supported the role of the overall hydrophobic character and of the large content of bioavailable lignin units and nitrogen moieties. These results confirm that the combination of solid state NMR and thermochemolysis are valuable and efficient tools to assess the molecular quality of natural organic matter. The detailed molecular characterization of compost materials may hence represent a useful requirement to select the most suitable application of bulk biomasses and derived fractions for soil organic matter managements. MenosThe molecular characteristics of different vermicomposts, produced with variable rates of cattle manure and cotton residues, and of related humic acids were determined by 13C-CPMAS NMR and Off-line pyrolysis THM-GC?MS. The analyses highlighted a composition of mature vermicomposts based on differential amounts of lignocellulosic fraction and alkyl compounds inherited from original biomasses, thus revealing a decrease of overall hydrophobic character at increasing amounts of cotton input. The evaluation of molecular parameters derived from both solid state NMR spectra and from the specific biomarkers released by thermochemolysis suggested that the humification process was mainly characterized by the selective preservation of specific lignin derivatives. The potential bioactive properties of humic acids were hence evaluated in a bioassays test on maize seedlings. The humic extracts showed a differential response in root growth-promoting effect, depending on the specific molecular features. The higher bioactivity found in humic components isolated from vermicompost with no or low addition of cotton residues, supported the role of the overall hydrophobic character and of the large content of bioavailable lignin units and nitrogen moieties. These results confirm that the combination of solid state NMR and thermochemolysis are valuable and efficient tools to assess the molecular quality of natural organic matter. The detailed molecular characterization of compost materials may hen... Mostrar Tudo |
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Palavras-Chave: |
Cotton residues Humic extracts; Cow manure; Molecular characterization; Structura ctivity; Vermicompost. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02560naa a2200277 a 4500
001 2110756
005 2019-11-19
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jaap.2019.104634$2DOI
100 1 $aAQUINO, A. M. de
245 $aEvaluation of molecular properties of humic acids from vermicompost by 13 C CPMAS NMR spectroscopy and thermochemolysis GC MS$h[electronic resource]
260 $c2019
520 $aThe molecular characteristics of different vermicomposts, produced with variable rates of cattle manure and cotton residues, and of related humic acids were determined by 13C-CPMAS NMR and Off-line pyrolysis THM-GC?MS. The analyses highlighted a composition of mature vermicomposts based on differential amounts of lignocellulosic fraction and alkyl compounds inherited from original biomasses, thus revealing a decrease of overall hydrophobic character at increasing amounts of cotton input. The evaluation of molecular parameters derived from both solid state NMR spectra and from the specific biomarkers released by thermochemolysis suggested that the humification process was mainly characterized by the selective preservation of specific lignin derivatives. The potential bioactive properties of humic acids were hence evaluated in a bioassays test on maize seedlings. The humic extracts showed a differential response in root growth-promoting effect, depending on the specific molecular features. The higher bioactivity found in humic components isolated from vermicompost with no or low addition of cotton residues, supported the role of the overall hydrophobic character and of the large content of bioavailable lignin units and nitrogen moieties. These results confirm that the combination of solid state NMR and thermochemolysis are valuable and efficient tools to assess the molecular quality of natural organic matter. The detailed molecular characterization of compost materials may hence represent a useful requirement to select the most suitable application of bulk biomasses and derived fractions for soil organic matter managements.
653 $aCotton residues Humic extracts
653 $aCow manure
653 $aMolecular characterization
653 $aStructura ctivity
653 $aVermicompost
700 1 $aCANELLAS, L. P.
700 1 $aSILVA, A. P. S. da
700 1 $aCANELLAS, N. O.
700 1 $aLIMA, L. da S.
700 1 $aOLIVARES, F. L.
700 1 $aPICCOLO, A.
700 1 $aSPACCINI, R.
773 $tJournal of Analytical and Applied Pyrolysis, v .141, 104634, August 2019.
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