|
|
Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
08/09/2022 |
Data da última atualização: |
08/09/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, E. M.; ROMERO, L. C.; CUNHA, M. DE L. R. DE S. DA; MALAGO JUNIOR, W.; CAMARGO, C. H.; BARIONI JUNIOR, W.; ZAFALON, L. F. |
Afiliação: |
ELKA MACHADO FERREIRA, UNESP; LETÍCIA CASTILHO ROMERO, UNESP; MARIA DE LOURDES RIBEIRO DE SOUZA DA CUNHA, UNESP; WILSON MALAGO JUNIOR, CPPSE; CARLOS HENRIQUE CAMARGO, Bacteriology Center, Adolfo Lutz Institute; WALDOMIRO BARIONI JUNIOR, CPPSE; LUIZ FRANCISCO ZAFALON, CPPSE. |
Título: |
Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
BMC Veterinary Research, v. 18, n. 273, 2022. |
Páginas: |
13 p. |
DOI: |
https://doi.org/10.1186/s12917-022-03364-8 |
Idioma: |
Inglês |
Conteúdo: |
Background: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds. MenosBackground: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolatio... Mostrar Tudo |
Palavras-Chave: |
Clonal profile; IcaADBC. |
Thesaurus Nal: |
Biofilm. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146229/1/PersistenceStaphylococcusMilk.pdf
|
Marc: |
LEADER 03109naa a2200253 a 4500 001 2146229 005 2022-09-08 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s12917-022-03364-8$2DOI 100 1 $aFERREIRA, E. M. 245 $aPersistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis.$h[electronic resource] 260 $c2022 300 $a13 p. 520 $aBackground: Mastitis is one of the major diseases in dairy cattle, as it causes great economic losses to producers due to the reduction of milk production and changes in the quality of the product. The disease is mainly caused by bacteria of the genus Staphylococcus spp., these microorganisms can express various virulence factors, such as biofilms for example. In herds with organic management, producers and technicians use unconventional ways to treat and control the disease, such as homeopathy. However, it is not known if this type of treatment is able to control pathogenic bacteria such as those of the genus Staphylococcus, of relevance to animal and human health. Thus, the objective of this study was to investigate the production of biofilm in vitro and its genes by Staphylococcus spp. isolated in the milk of cows treated with homeopathy, as well as the persistence of microorganisms in animals. Methods: Ninety-nine isolates of Staphylococcus spp. from cows treated and not treated with homeopathy were identified by internal transcribed space-polymerase chain reaction and investigated for the presence of the icaABCD, bap, aap, atlE, and bhp genes and in vitro biofilm production using the adhesion method on polystyrene plates. The enzyme restriction profile was determined by Pulsed-Field Gel Electrophoresis. Clusters of S. aureus and S. epidermidis with three or more isolates had an isolate selected for Multilocus Sequence Typing. Results: The frequency of S. aureus isolations was similar in treated and untreated cows, while 71.4% of the coagulase-negative identified were isolated in cows treated with homeopathy. The distribution of the operon ica genes was similar in animals with and without treatment, except for the icaD gene, more frequent in treated cows. Production of biofilm was associated with presence of one or more genes from the icaADBC operon. S. aureus revealed a greater diversity and greater dissemination in cows treated and not treated with homeopathy. Sequence Types ST1, ST5, and ST126 were identified in S. aureus. Conclusions: The presence of biofilm-associated genes and the in vitro production of biofilms, combined with the persistence of clonal profiles of Staphylococcus spp. demonstrate other forms of control for bovine mastitis should be researched for organic production herds. 650 $aBiofilm 653 $aClonal profile 653 $aIcaADBC 700 1 $aROMERO, L. C. 700 1 $aCUNHA, M. DE L. R. DE S. DA 700 1 $aMALAGO JUNIOR, W. 700 1 $aCAMARGO, C. H. 700 1 $aBARIONI JUNIOR, W. 700 1 $aZAFALON, L. F. 773 $tBMC Veterinary Research$gv. 18, n. 273, 2022.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registros recuperados : 11 | |
3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARTINS, K. B.; FACCIOLI, P. Y.; BONESSO, M. F.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Characteristics of resistance and virulence factors in different species of coagulase-negative staphylococci isolated from milk of healthy sheep and animals with subclinical mastitis. Journal of Dairy Science, v. 100, n. 3, p. 2184-2195, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARLIN, K. B.; PEREIRA, V. C.; OLIVEIRA, A. de; FERNANDES, S. J.; FACCIOLI, P. Y.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Evaluation of the presence of the bap gene in staphylococcus isolated from ewe subclinical mastitis milk. In: INTERNATIONAL SYMPOSIUM ON STAPHYLOCOCCI AND STAPHYLOCOCCAL INFECTIONS, 2012, Lyon. Abstract... Lyoon: Universite de Lyon, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MELLO, P. L.; RIBOLI, D. F. M.; PINHEIRO, L.; MARTINS, L. de A.; BRITO, M. A. V. P. e; CUNHA, M. de L. R. de S. da. Detection of enterotoxigenic potential and determination of clonal profile in Staphylococcus aureus and Coagulase-Negative Staphylococci isolated from bovine subclinical mastitis in different Brazilian states. Toxins, v. 8, n. 4, article 104, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MELLO, P. L.; FACCIOLI-MARTINS, P. Y.; LAMEIDA M. L. de; BRITO, M. A. V. P.; CUNHA, M. de L. R. de S. da. Determination of resistance and virulence in Staphylococcus spp. Isolated from bovine mastitis in different Brazilian states Hechos Microbiológicos, v. 5, n. 2, p. 114-115, 2014. Suplemento. Resumen TLP-543. Edição das Memorias do 22º Congreso Latinoamericano de Microbiologia (ALAM) e 4º Congreso Colombiano de Microbiologia, Cartagena, 2014.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PIRES, F. V.; CUNHA, M. de L. R. de S. da; ABRAÃO, L. M.; FACCIOLI-MARTINS, P. Y.; CAMARGO, C. H.; FORTALEZA, C. M. C. B. Nasal carriage of Staphylococcus aureus in Botucatu, Brazil: a population-based survey. PLOS ONE, v. 9, n. 3, e92537, Marc. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso restrito ao objeto digital](/consulta/web/img/lock.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, E. M.; ROMERO, L. C.; CUNHA, M. DE L. R. DE S. DA; MALAGO JUNIOR, W.; CAMARGO, C. H.; BARIONI JUNIOR, W.; ZAFALON, L. F. Persistence of Staphylococcus spp. in milk from cows undergoing homeopathy to control subclinical mastitis. BMC Veterinary Research, v. 18, n. 273, 2022. 13 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ZAFALON, L. F.; CUNHA, M. de L. R. de S. da; TEIXEIRA, N. B.; GARCES, H. G.; YAMAUCHI, D. H.; SILVA, L. P.; FERREIRA, E. M. Toxin-encoding genes of Staphylococcus Spp. Isolated from milk of cows treated with homeopathy. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 30., 2019, Maceió, Anais... Maceió: SBM, 2019.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARTINS, K. B.; FACCIOLI-MARTINS, P. Y.; RIBOLI, D. F. M.; PEREIRA, V. C.; FERNANDES, S.; OLIVEIRA, A. A.; DANTAS, A.; ZAFALON, L. F.; CUNHA, M. de L. R. de S. da. Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk. Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, apr./jun. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, E. M.; ROMERO, L. C.; SILVA, L. P.; PEREIRA, L. E. C.; MALAGO JUNIOR, W.; CHAPAVAL, L.; CUNHA, M. de L. R. de S. da; ZAFALON, L. F. Genotypic and phenotypic characterization of antimicrobial resistance in Staphylococcus Spp. isolated from cows after alternative treatment of bovine mastitis. In: INTERNATIONAL SYMPOSIUM ON VETERINARY SCIENCE, 2019, Viçosa. Proceedings... Viçosa: UFV, 2019. p.164Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
| ![Visualizar detalhes do registro](/consulta/web/img/visualizar.png) ![Acesso ao objeto digital](/consulta/web/img/pdf.png) ![Imprime registro no formato completo](/consulta/web/img/print.png) |
Registros recuperados : 11 | |
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|