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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/09/2015 |
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Data da última atualização: |
01/03/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, C. G.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
HEIDI CHRISTINA BESSLER CUMPA, CPAC; ALVARO MORAES DA FONSECA NETO, CPAC; CARLOS FREDERICO MARTINS, CPAC; UNB. |
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Título: |
Effect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Acetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type. MenosAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and... Mostrar Tudo |
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Palavras-Chave: |
Cloning; Epigenetic. |
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Thesagro: |
Clonagem. |
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Thesaurus Nal: |
histone deacetylase. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129960/1/34712.pdf
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Marc: |
LEADER 03483nam a2200205 a 4500
001 2024379
005 2016-03-01
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVA, C. G.
245 $aEffect of trichostatin a in cloned cattle embryo production by nuclear transfer with mesenchymal stem cells.
260 $aAnimal Reproduction, v. 12, n. 3, p. 812, Jul./Sept. 2015.$c2015
520 $aAcetylation of histones is a major mechanism of genome epigenetic reprogramming of the gametes in order to establish a totipotent state to the normal development (Ikeda et al., Zygote 17, 209-215, 2009). The deacetylation is catalysed by histone deacetylases (HDAC) which remove acetyl groups and causes chromatin compaction and DNA segment silencing at this location (Johnstone, Nature Reviews Drug Discovery 1, 287-299, 2002). The trichostatin A (TSA) is an HDAC inhibitor that increases the amount of acetylated histones and the demethylation of DNA (Lee et al., Journal of Reproduction and Development 57, 34-42, 2011). In this sense, the drug has been used in an attempt to increase the production efficiency of embryos by nuclear transfer (NT). The objective of this study was to test the effect of TSA in exposure times of 20 and 25 hours in the culture of bovine embryos cloned by NT with mesenchymal stem cells (MSCs) derived from adipose tissue. A biopsy of skin and adipose tissue was collected from the perineal region of a female bovine Gir, at two months of age. The cells were isolated by the explant and cultured in Dulbeccos Modified Eagle Medium added with 10% fetal bovine serum. Cumulus-oocyte complexes recovered from slaughterhouse ovaries were matured for 18 h at 38.5°C and 5% CO2. The NT was then performed with the adipose tissue and the MSCs reconstructed embryos were subjected to culture with 50 nM TSA for 20 and 25 h, for 4 h in activation medium containing 6-DMAP and further for 16 or 21 h in medium cultivation. Subsequently, the embryos continued in culture in synthetic oviductal fluid (SOF) medium without TSA and the parthenogenetic control was performed in every manipulation. Five NT procedures were performed for each treatment (20 h, 25 h and without TSA). Fusion rates, cleavage and blastocyst production were compared by Tukey test (p <0.05). The cleavage rate of parthenogenetic embryos (93.45 ± 7.97) was higher than the cleavage rate of embryos without treatment (82.45 ± 5.59) but was statistically similar to embryos treated with 20 and 25 h (87.25 ± 8.41 and 85.54 ± 3.88, respectively). Still, there was no difference in cleavage rate between treated and untreated embryos. The blastocyst production rate on the seventh day of culture was superior to the parthenogenetic control (59.24 ± 11.75) compared to treatments for 20 h (36.22 ± 16.80), 25 h (33.66 ± 12.84) and without the use of TSA (32.70 ± 9.11), which did not differ. It can be concluded that the use of TSA had no significant effect in improving the rates of cleavage and development of bovine embryos by nuclear transfer with MSCs from adipose tissue. However, additional studies to evaluate the quality and embryos of the methylation pattern should be conducted to better understand the effects of TSA in embryos cloned with this cell type.
650 $ahistone deacetylase
650 $aClonagem
653 $aCloning
653 $aEpigenetic
700 1 $aCUMPA, H. C. B.
700 1 $aFONSECA NETO, A. M. da
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 19 | |
| 1. |  | ARAUJO, J. M.; OLIVEIRA, R. A.; CUMPA, H. C. B.; MARTINS, C. F. Effect of long cooling periods of the ear skin at 5°C on the isolation and culture of bovine fibroblasts. Animal Reproduction, v. 15, n. 3, p. 366, Jul./Sept. 2018. Abstract A051. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
|    |
| 3. | | SILVA, C.; MARTINS, C. F.; CUMPA, H. C. B.; MENDONÇA, A.; FRANCO, M. M.; BAO, S. Global methylation of bovine cells derived from amniotic fluid, adipose tissue and skin fibroblastos and its use in nuclear transfer in the presence or absence of Trichostatin A. In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, In Tours. Proceedings... In Tours, France: INCAR, 2016. p. 445.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | SILVA, C.; MARTINS, C. F.; CUMPA, H. C. B.; MENDONÇA, A.; FRANCO, M. M.; BAO, S. Global methylation of bovine cells derived from amniotic fluid, adipose tissue and skin fibroblastos and its use in nuclear transfer in the presence or absence of Trichostatin A. In: INTERNATIONAL CONGRESS OF ANIMAL REPRODUCTION, 18., 2016, In Tours. Proceedings... In Tours, France: INCAR, 2016. p. 445.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 6. | | CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
|  |
| 7. | | MARTINS, C. F.; CUMPA, H. C. B.; CUNHA, E. R. da; SILVA, C. G. da; BORGES, L. A.; GAMA FILHO, J. B. da. Isolamento, cultivo criopreservação de células somáticas de mamíferos silvestres para formação de um banco de germoplasma. Planaltina, DF: Embrapa Cerrados, 2013. 4 p. (Embrapa Cerrados. Comunicado Técnico, 175).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Cerrados. |
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| 8. | | SILVA, C. G. da; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MCMANUS, C. M.; PIVATO, I.; BAO, S. N. Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer. Isolamento e caracterização de células tronco mesenquimais derivadas de geléia de Wharton bovina e seu potencial para uso na clonagem por transferência nuclear. Ciência Rural, Santa Maria, v. 46, n. 10, p. 1830-1837, out. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; MENDONÇA, A. dos S.; FRANCO, M. M.; BÁO, S. N. Metilação e hidroximetilação global de células bovinas derivadas do fluido aminiótico, tecido adiposo e fibroblastos da pele e seu uso na clonagem na presença ou ausência de tricostatina A. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS. Jovens talentos 2016: resumos. Planaltina, DF: Embrapa Cerrados, 2016. 99 p. p. 29 (Embrapa Cerrados. Documentos, 334). Comissão organizadora: SOUZA, K. W. de; FALEIRO, A. S. G.; SALGUES, A. L. M.; ALONSO, A. M.; CARVALHO, A. M. de; LOBATO, B. R.; CRUZ, C. V.; SOUSA, E. dos S.; PELEGRINELLI, F.; SOARES, J. P. G.; LIMA, J. E. F. W.; ARBUES, J. F. de O.;...| Biblioteca(s): Embrapa Cerrados. |
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| 10. | | MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. Cryobiology, v. 71, p. 47-53, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | MARTINS, C. F.; FELICIANO SILVA, A. E. D.; DODE, M. A. N.; RUMPF, R.; CUMPA, H. C. B.; SILVA, C. G.; PIVATO, I. Morphological characterization and conservation of bovine spermatogenic cells by refrigeration at 4°C and freezing using different cryoprotective molecules. Cryobiology, v. 71, p. 47-53, 2015. p. 47-53| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| 12. | | SILVA, C. G.; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MARTINS, G. H. L.; PIVATO, I.; BÁO S. N. Production of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue. Cellular Reprogramming, v. 18, n. 2, p. 127-136, 2016. p. 127-136| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | FIGUEIREDO, P. O.; AZAMBUJA, G. G.; BARROS, L. C.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; PIVATO, I.; MARTINS, C. F. Ultrasound evaluation of ovarian dynamics in Indubrasil cows submitted to two nutritional managements. Animal Reproduction, v. 12, n. 3, p. 571, Jul./Sept. 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. Theriogenology, Vv. 160, p. 134-141, 2021. p. 134-141| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | SILVA, C. G. da; BÁO, S. N.; CUMPA, H. C. B.; CARDOSO, T. C.; FONSECA, L. O. da; FONSECA NETO, A. M. da; GODOY, S. D.; MARTINS, G. H.; CUNHA, E. R. da; MARTINS, C. F. Brasília da Cerrados: primeiro bovino clonado utilizando células-tronco mesenquimais do tecido adiposo. Planaltina, DF: Embrapa Cerrados, 2016. 34 p. (Embrapa Cerrados. Documentos, 330)| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | MARTINS, C. F.; FERREIRA, I. C.; TOLEDO, M. R. de; MACHADO, C. H. C.; FONSECA NETO, A. M. da; BALBINO, L. C.; GODOY, S. D.; PACHECO, G. R.; CUMPA, H. C. B. Primeira prova brasileira de produção de leite a pasto do Zebu Leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras (CTZL) da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro Planaltina, DF: Embrapa Cerrados, 2016. 41 p. (Embrapa Cerrados. Documentos, 336).| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| 17. | | MARTINS, C. F.; FERREIRA, I. C.; FONSECA NETO, A. M. da; TOLEDO, M. R. de; OJEDA, D. B.; GODOY, S. D.; PACHECO, G. R.; CUMPA, H. C. B.; BORGES, J. R. J.; BALBINO, L. C. Segunda prova brasileira de produção de leite a pasto do zebu leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras da Embrapa Cerrados: caderno de resultados para vacas e novilhas da raça Gir Leiteiro e para novilhas Sindi. Planaltina, DF: Embrapa Cerrados, 2018. 55 p. (Embrapa Cerrados. Documentos, 345).| Biblioteca(s): Embrapa Cerrados. |
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| 18. | | FONSECA NETO, A. M. da; MARTINS, C. F.; MIZIARA, F.; PACHECO, G. R.; CUMPA, H. C. B.; FERREIRA, I. C.; BORGES, J. R. J.; BALBINO, L. C.; TOLETO, M. R. de; SILVA, P. M. R. S. da; GODOY, S. D. Terceira prova brasileira de produção de leite a pasto do Zebu Leiteiro no Centro de Tecnologias para Raças Zebuínas Leiteiras (CTZL) da Embrapa Cerrados: caderno de resultados para novilhas da raça Gir Leiteiro Planaltina, DF: Embrapa Cerrados, 2019. 41 p. (Embrapa Cerrados. Documentos, 351).| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; FONSECA NETO, A. M. da; CARDOSO, T. C.; FRANCO, M. M.; MENDONÇA, A. dos S.; LEME, L. de O.; BORGES, J. R. J.; MALAQUIAS, J. V.; BAO, S. N. Use of trichostatin A alters the expression of HDAC3 and KAT2 and improves in vitro development of bovine embryos cloned using less methylated mesenchymal stem cells. Reproduction in Domestic Animals, v. 54, n. 2, p. 289-299, February 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 19 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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