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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
05/02/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. |
Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, UNESP; MARIA HELENA COELHO CRUZ, UNESP; BRUNA MAZETI, UNESP; LETICIA ZOCCOLARO OLIVEIRA, UNESP; FLAVIA LOMBARDI LOPES, UNESP; JOAQUIM MANSANO GARCIA, UNESP. |
Título: |
HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Reproduction, v. 145, n. 1, p. 9-17, 2013. |
DOI: |
https://doi.org/10.1530/rep-11-0343 |
Idioma: |
Inglês |
Conteúdo: |
During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos. MenosDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained u... Mostrar Tudo |
Palavras-Chave: |
Cultured Female Fertilization; Drug effects. |
Thesaurus Nal: |
Blastocyst; Embryology; Metabolism. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02665naa a2200265 a 4500 001 1978655 005 2024-02-05 008 2013 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1530/rep-11-0343$2DOI 100 1 $aOLIVEIRA, C. S. 245 $aHDAC inhibition decreases XIST expression on female IVP bovine blastocysts.$h[electronic resource] 260 $c2013 520 $aDuring initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, although in vitro culture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) on female preimplantation development. We tested the hypothesis that by enhancing histone acetylation, TSA would increase the percentage of embryos achieving 16-cell stage, reducing percentage of embryos blocked at 8-cell stage, and interfere with XCI in IVF embryos. We noticed that after TSA treatment, acetylation levels in individual blastomeres of 8-16 cell embryos were increased twofold on treated embryos, and the same was detected for blastocysts. Changes among blastomere levels within the same embryo were diminished on TSA group, as low-acetylated blastomeres were no longer detected. The percentage of embryos that reached the 5th cleavage cycle 118 h after IVF, analyzed by Hoechst staining, remained unaltered after TSA treatment. Then, we assessed XIST and G6PD expression in individual female bovine blastocysts by quantitative real-time PCR. Even though G6PD expression remained unaltered after TSA exposure, XIST expression was eightfold decreased, and we also detected a major decrease in the percentage of blastocysts expressing detectable XIST levels after TSA treatment. Based on these results, we conclude that HDAC is involved on XCI process in bovine embryos, and its inhibition might delay X chromosome silencing and attenuate aberrant XIST expression described for IVF embryos. 650 $aBlastocyst 650 $aEmbryology 650 $aMetabolism 653 $aCultured Female Fertilization 653 $aDrug effects 700 1 $aSARAIVA, N. Z. 700 1 $aCRUZ, M. H. C. 700 1 $aMAZETI, B. 700 1 $aOLIVEIRA, L. Z. 700 1 $aLOPES, F. L. 700 1 $aGARCIA, J. M. 773 $tReproduction$gv. 145, n. 1, p. 9-17, 2013.
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Registros recuperados : 16 | |
4. | | VALENCIA, B. B. M.; CRUZ, M. H.; BONILLA, J. L. S.; COLMENERO, A. Z. Calidad agroindustrial del aceite de higuerilla (Ricinus communis L.) para la producción de bioenergéticos en Mexico. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 2Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Algodão. |
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8. | | MOREIRA, M. M.; FERREIRA, G. B.; SILVA, M. S. L. da; CRUZ, M. H. L.; ROCHA, J. C. da. Barragem subterrânea e sua influência no redesenho de agroecossitemas no agreste paraibano. Cadernos de Agroecologia, v. 4, n. 1, p. 3546-3549, 2009. Trabalho apresentado no 6º Congresso Brasileiro de Agroecologia e 2º Congresso Latinoamericano de Agroecologia, 2009, Curitiba.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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10. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; DEL COLLADO, M.; LIMA, M. R.; CRUZ, M. H.; VANTINI, R.; GARCIA, J. M. Efeitos da pré-maturação in vitro de oócitos bovinos com moduladores de AMPc sobre a progressão nuclear. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Leite. |
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11. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; CRUZ, M. H. C.; MAZETI, B.; OLIVEIRA, L. Z.; LOPES, F. L.; GARCIA, J. M. HDAC inhibition decreases XIST expression on female IVP bovine blastocysts. Reproduction, v. 145, n. 1, p. 9-17, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Gado de Leite. |
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12. | | CRUZ, M. H. C.; SARAIVA, N. Z.; CRUZ, J. F. da; OLIVEIRA, C. S.; COLLADO, M. del; FERNANDES, H.; CASTRO, F. C. de; GARCIA, J. M. Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro. Revista Brasileira de Zootecnia, v. 43, n. 3, p. 120-126, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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13. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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15. | | BANDEIRA, R. D. C. C.; UEKANE, T. M.; CUNHA, C. P. da; GEAQUINTO, L. R. O.; CUNHA, V. S.; CAIXEIRO, J. M. R.; GODOY, R. L. O.; CRUZ, M. H. C. de la. Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS). Química Nova, São Paulo, v. 35, n. 1, p. 66-71, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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16. | | BANDEIRA, R. D. da C. C.; UEKANE, T. M.; CUNHA, C. P. da; COSTA, D. A.; DE LA CRUZ, M. H. C.; GODOY, R. L. de O.; CUNHA, V. S.; RODRIGUES, J. M. Development of method for Ochratoxin A analysis in coffee by liquid chromatography/electrospray tandem mass spectrometry. In: SIMPOSIO DE METROLOGÍA, 2008, Santiago de Querétaro, México. Diseminando la cultura de la medición en México: sesiones murales. Santiago de Querétaro, México: Centro Nacional de Metrología, 2008. Sesión SM1.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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Registros recuperados : 16 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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