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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
23/01/2019 |
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Data da última atualização: |
23/01/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
PCR-based genotyping of SNP markers in sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 4, p. 651-654, Aug. 2018. |
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DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
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Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
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Thesagro: |
Marcador Molecular; Ovino. |
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Thesaurus Nal: |
Genome. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02277naa a2200277 a 4500
001 2104694
005 2019-01-23
008 2018 bl --- 0-- u #d
024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource]
260 $c2018
520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.
650 $aGenome
650 $aMarcador Molecular
650 $aOvino
653 $aPCR RFLP
653 $aResistência nematódeo gastrintestinal
653 $aTetra primer ARMS PCR
700 1 $aCRUVINEL, G. G.
700 1 $aMORAES, C. V.
700 1 $aDONATONI, F. A. B.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aCHAGAS, A. C. de S.
773 $tMolecular Biology Reports$gv. 45, n. 4, p. 651-654, Aug. 2018.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
03/12/2009 |
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Data da última atualização: |
19/04/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABDELNOOR, R. V.; NEPOMUCENO, A. L.; BARROS, E. G. de; SA, M. de F. G. de; BINNECK, E.; MARCELINO, F. C.; BROMMONSCHENKEL, S. H.; ALMEIDA, J.; ISEPPON, A. M. B.; SCHUSTER, I.; KIDO, E. A.; LOUREIRO, M. E.; MARGIS, R.; HUNGRIA, M.; MOREIRA, M. A.; BACARAT-PEREIRA, M. C.; FIETTO, L. G.; BODANESE-ZANETTINI, M. H.; ROMANO, E.; ZERBINI, F. M.; LEMOS, E. G. de M.; PEREIRA, G. A. G. |
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Afiliação: |
RICARDO VILELA ABDELNOOR, CNPSo; ALEXANDRE LIMA NEPOMUCENO, CNPSo; E. G. DE BARROS, UFV; MARIA FATIMA GROSSI DE SA, CENARGEN; ELISEU BINNECK, CNPSo; FRANCISMAR CORREA MARCELINO, CNPSo; S. H. BROMMONSCHENKEL, UFV; JULIANA DANTAS DE ALMEIDA, CENARGEN; A. M. B. ISEPPON, Universidade de Pernambuco; I. SHUSTER, COODETEC; E. A. KIDO, Universidade de Pernambuco; M. E. LOUREIRO, UFV; R. MARGIS, UFRGS; MARIANGELA HUNGRIA DA CUNHA, CNPSo; M. A. MOREIRA, UFV; M. C. BACARAT-PEREIRA, UFV; L. G. FIETTO, UFV; M. H. BODANESE-ZANETTINI, UFRGS; EDUARDO ROMANO DE CAMPOS PINTO, CENARGEN; F. M. ZERBINI, UFV; E. G. DE MACEDO LEMOS, UNESP; G. A. G. PEREIRA, UNICAMP. |
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Título: |
GENOSOJA - A brazilian soybean genome consortium. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 108, ref. O201. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng. |
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Idioma: |
Inglês |
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Palavras-Chave: |
Consórcio. |
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Thesagro: |
Genoma; Soja. |
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Thesaurus NAL: |
Genome; Soybeans. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 01479naa a2200421 a 4500
001 1577260
005 2010-04-19
008 2009 bl uuuu u00u1 u #d
100 1 $aABDELNOOR, R. V.
245 $aGENOSOJA - A brazilian soybean genome consortium.
260 $c2009
650 $aGenome
650 $aSoybeans
650 $aGenoma
650 $aSoja
653 $aConsórcio
700 1 $aNEPOMUCENO, A. L.
700 1 $aBARROS, E. G. de
700 1 $aSA, M. de F. G. de
700 1 $aBINNECK, E.
700 1 $aMARCELINO, F. C.
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aALMEIDA, J.
700 1 $aISEPPON, A. M. B.
700 1 $aSCHUSTER, I.
700 1 $aKIDO, E. A.
700 1 $aLOUREIRO, M. E.
700 1 $aMARGIS, R.
700 1 $aHUNGRIA, M.
700 1 $aMOREIRA, M. A.
700 1 $aBACARAT-PEREIRA, M. C.
700 1 $aFIETTO, L. G.
700 1 $aBODANESE-ZANETTINI, M. H.
700 1 $aROMANO, E.
700 1 $aZERBINI, F. M.
700 1 $aLEMOS, E. G. de M.
700 1 $aPEREIRA, G. A. G.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 108, ref. O201. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.
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Embrapa Soja (CNPSO) |
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