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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
22/03/2016 |
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Data da última atualização: |
22/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, N. R.; ALMEIDA, R. P. de; PADILHA, I. Q. M.; ARAÚJO, D. A. M.; CREÃO-DUARTE, A. J. |
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Afiliação: |
N. R. SANTOS, UFPB; RAUL PORFIRIO DE ALMEIDA, CNPA; I. Q. M. PADILHA, UFPB; D. A. M. ARAÚJO, UFPB; A. J. CREÃO-DUARTE, UFPB. |
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Título: |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Brazilian Journal of Biology, v. 75, n. 2, p. 391-395, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species. |
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Palavras-Chave: |
DNA ribossomal; Microhymenoptera; Parasitoid. |
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Thesagro: |
DNA. |
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Thesaurus Nal: |
Trichogramma. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141508/1/Molecualr-identification-of-Trichogramma-....pdf
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Marc: |
LEADER 01819naa a2200229 a 4500
001 2041586
005 2016-03-22
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, N. R.
245 $aMolecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA.$h[electronic resource]
260 $c2015
520 $aThe objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species.
650 $aTrichogramma
650 $aDNA
653 $aDNA ribossomal
653 $aMicrohymenoptera
653 $aParasitoid
700 1 $aALMEIDA, R. P. de
700 1 $aPADILHA, I. Q. M.
700 1 $aARAÚJO, D. A. M.
700 1 $aCREÃO-DUARTE, A. J.
773 $tBrazilian Journal of Biology$gv. 75, n. 2, p. 391-395, 2015.
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Embrapa Algodão (CNPA) |
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