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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
23/03/2022 |
Data da última atualização: |
24/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
COUTINHO, T. C.; MALAFATTI, J. O. D.; PARIS, E. C.; TARDIOLI, P. W.; FARINAS, C. S. |
Afiliação: |
ELAINE CRISTINA PARIS, CNPDIA; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Título: |
Hydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
ACS Applied Nano Materials, v. 3, 2020. |
Páginas: |
12334–12345 |
DOI: |
https://dx.doi.org/10.1021/acsanm.0c02811 |
Idioma: |
Inglês |
Conteúdo: |
Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining MenosHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes... Mostrar Tudo |
Palavras-Chave: |
Cobalt ferrite; Immobilization; Magnetic nanoparticles. |
Categoria do assunto: |
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Marc: |
LEADER 02369naa a2200229 a 4500 001 2141192 005 2024-01-24 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://dx.doi.org/10.1021/acsanm.0c02811$2DOI 100 1 $aCOUTINHO, T. C. 245 $aHydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery.$h[electronic resource] 260 $c2022 300 $a12334–12345 520 $aHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining 653 $aCobalt ferrite 653 $aImmobilization 653 $aMagnetic nanoparticles 700 1 $aMALAFATTI, J. O. D. 700 1 $aPARIS, E. C. 700 1 $aTARDIOLI, P. W. 700 1 $aFARINAS, C. S. 773 $tACS Applied Nano Materials$gv. 3, 2020.
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8. | | LOPES, M. M.; COUTINHO, T. C.; FARINAS, C. S. Imobilização da fitase em zeólita modificada com níquel: uma ferramenta para melhoria da atividade catalítica. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 602-606Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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10. | | COUTINHO, T. C.; TARDIOLI, P. W.; FARINAS, C. S. Melhoria nas propriedades da fitase após sua imobilização em hidroxiapatita para aplicação em ração animal. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 570-574Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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18. | | COUTINHO, T. C.; ROJAS, M. J.; PARIS, E. C.; FARINAS, C. S. Imobilização de beta-glicosidase em nanopartículas de hidroxiapatita. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 223-226. Editores: Caue Ribeiro de Oliveira, Elaine Cristina Paris, Luiz Henrique Capparelli Mattoso, Marcelo Porto Bemquerer, Maria Alice Martins, Odílio Benedito Garrido de Assis.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Instrumentação. |
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