Data/hora: 19/06/2024 21:51:55

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 13/10/2010
Data da última atualização: | 30/04/2018
Autoria: | OLIVEIRA, M. L. P. de; COSTA, M. G. C.; SILVA, C. V. da; OTONI, W. C.
Afiliação: | Maria Luiza Peixoto de Oliveira, Universidade Federal de Viçosa; Marcio Gilberto Cardoso Costa, Universidade Estadual de Santa Cruz; Crislene Viana da Silva, Universidade Federal de Viçosa; Wagner Campos Otoni, Universidade Federal de Viçosa.
Título: | Growth regulators, culture media and antibiotics in the in vitro shoot regeneration from mature tissue of citrus cultivars.
Ano de publicação: | 2010
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 45, n. 7, p.654-660, jul. 2010
Idioma: | Inglês
Notas: | Título em português: Reguladores de crescimento, meio de cultura e antibióticos na regeneração in vitro de tecidos maduros de cultivares de citros.
Conteúdo: | The objective of this study was to evaluate the effects of 6-benzylaminopurine (BAP) and ?-naphthaleneacetic acid (NAA) combinations, basal media and beta-lactam antibiotics on in vitro organogenesis from mature stem segments of 'PÛra', 'ValÛncia' and 'Bahia' sweet oranges and 'Cravo' rangpur lime. For induction of shoot regeneration, the segments of the four cultivars were placed on Murashige and Skoog (MS) medium containing the following BAP/NAA concentrations: 0.0/0.0; 0.25/0.0; 0.25/0.25; 0.5/0.0; 0.5/0.5; 1.0/0.0; 2.0/0.0; 2.0/0.25; 2.0/0.5; and 2.0/1.0ámgáL-1. In order to test the influence of the culture media on shoot-bud induction, (MS), Murashige and Tucker (MT), and woody plant medium (WPM) formulations were evaluated, associated with the best combination of plant growth regulators obtained in the previous experiment. The influence of four beta-lactam antibiotics (timentin, cefotaxime sodium salt, meropenem trihydrate and augmentin) on shoot regeneration was determined. Better regeneration responses were achieved when internodal segments were cultured onto MS-based medium with 500 mg L-1 cefotaxime with the following BAP/NAA concentrations: 0.5 + 0.25 mg L-1 for 'Cravo', 1.0 + 0.25 mg L-1 for 'Valência' and 'Bahia', and 1.0 + 0.5 mg L-1 for 'Pêra'. Genotype, growth regulators, basal media and beta-lactam antibiotics affect the morphogenetic response in mature tissues of citrus.
Palavras-Chave: | Adult tissues; C sinensis.
Thesagro: | Citrus Limonia.
Thesaurus Nal: | Antibiotics; Organogenesis.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/105846/1/Growth-regulators.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (48293-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS