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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/12/2015 |
Data da última atualização: |
20/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. |
Afiliação: |
DANIEL DIAS RUFINO ARCANJO, UNIVERSIDADE FEDERAL DO PIAUÍ; ANDREANNE GOMES VASCONCELOS, UNIVERSIDADE FEDERAL DO PIAUÍ; SIMÓN GABRIEL, AARHUS UNIVERSITY; JOILSON RAMOS JESUS, UNIVERSIDADE FEDERAL DO PIAUÍ; LUCIANO PAULINO DA SILVA, CENARGEN; OSMINDO RODRIGUES PIRES JÚNIOR, UNIVERSIDADE DE BRASÍLIA; CLAUDIO MIGUEL COSTA-NETO, UNIVERSIDADE DE SÃO PAULO; EDUARDO BRANDT OLIVEIRA, UNIVERSIDADE DE SÃO PAULO; LUDOVICO MIGLIOLO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; OCTÁVIO LUIZ FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; CAROLINA BARALDI ARAÚJO RESTINI, UNIVERSIDADE DE RIBEIRÃO PRETO; MICHELE PAULO, UNIVERSIDADE DE SÃO PAULO; LUSIANE MARIA BENDHACK, UNIVERSIDADE DE SÃO PAULO; MARCELO PORTO BEMQUERER, CENARGEN; ALDEIDIA PEREIRA OLIVEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; ULF SIMONSEN, AARHUS UNIVERSITY; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE FEDERAL DO PIAUÍ. |
Título: |
A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, v. 10, dez. 2015. (Open Access) |
DOI: |
10.1371/journal.pone.0145071 |
Idioma: |
Inglês |
Conteúdo: |
Proline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases. MenosProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of ne... Mostrar Tudo |
Palavras-Chave: |
Brachycephalus ephippium; Proline-rich oligopeptides. |
Thesaurus Nal: |
secretion. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182309/1/journal.pone.0145071.PDF
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Marc: |
LEADER 02864naa a2200361 a 4500 001 2031700 005 2024-06-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0145071$2DOI 100 1 $aARCANJO, D. D. R. 245 $aA novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium.$h[electronic resource] 260 $c2015 520 $aProline-rich oligopeptides (PROs) are a large family which comprises the bradykinin-potentiating peptides (BPPs). They inhibit the activity of the angiotensin I-converting enzyme (ACE) and have a typical pyroglutamyl (Pyr)/proline-rich structure at the N- and C-terminus, respectively. Furthermore, PROs decrease blood pressure in animals. In the present study, the isolation and biological characterization of a novel vasoactive BPP isolated from the skin secretion of the frog Brachycephalus ephippium is described. This new PRO, termed BPP-Brachy, has the primary structure WPPPKVSP and the amidated form termed BPPBrachyNH2 inhibits efficiently ACE in rat serum. In silico molecular modeling and docking studies suggest that BPP-BrachyNH2 is capable of forming a hydrogen bond network as well as multiple van der Waals interactions with the rat ACE, which blocks the access of the substrate to the C-domain active site. Moreover, in rat thoracic aorta BPP-BrachyNH2 induces potent endothelium-dependent vasodilatation with similar magnitude as captopril. In DAF-FM DA-loaded aortic cross sections examined by confocal microscopy, BPP-BrachyNH2 was found to increase the release of nitric oxide (NO). Moreover, BPP-BrachyNH2 was devoid of toxicity in endothelial and smooth muscle cell cultures. In conclusion, the peptide BPP-BrachyNH2 has a novel sequence being the first BPP isolated from the skin secretion of the Brachycephalidae family. This opens for exploring amphibians as a source of new biomolecules. The BPP-BrachyNH2 is devoid of cytotoxicity and elicits endothelium-dependent vasodilatation mediated by NO. These findings open for the possibility of potential application of these peptides in the treatment of endothelial dysfunction and cardiovascular diseases. 650 $asecretion 653 $aBrachycephalus ephippium 653 $aProline-rich oligopeptides 700 1 $aVASCONCELOS, A. G. 700 1 $aCOMERMA-STEFFENSEN, S. G. 700 1 $aJESUS, J. R. 700 1 $aSILVA, L. P. da 700 1 $aPIRES JÚNIOR, O. R. 700 1 $aCOSTA-NETO, C. M. 700 1 $aOLIVEIRA, E. B. 700 1 $aMIGLIOLO, L. 700 1 $aFRANCO, O. L. 700 1 $aRESTINI, C. B. A. 700 1 $aPAULO, M. 700 1 $aBENDHACK, L. M. 700 1 $aBEMQUERER, M. P. 700 1 $aOLIVEIRA, A. P. 700 1 $aSIMONSEN, U. 700 1 $aLEITE, J. R. de S. de A. 773 $tPlos One$gv. 10, dez. 2015. (Open Access)
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 4 | |
1. | | BRAND, G. D.; KRAUSE, F. C.; LEITE, J. R. S. A.; PRATES, M. V.; COSTA-NETO, C. M.; BLOCH JUNIOR, C. Mass spectrometric studies on novel bradykinin and phyllokinin analogs from the skin secretion of Phyllomedusa hypochondrialis. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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2. | | SILVA, L. P.; LEITE, J. R. S. A.; MELO, J. A. T.; OLIVEIRA, E. B.; PAVANELLI, W.; SILVA, J. S.; COSTA NETO, C. M.; BLOCH JÚNIOR, C. Phyllomelittins: novel antimicrobial peptides from the Brazilian treefrog Phyllomedusa hypochondrialis. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 35., 2006, Águas de Lindóia, SP. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2006. Não paginado.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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3. | | BRAND, G. D.; KRAUSE, F. C.; SILVA, L. P. da; LEITE, J. R. S. A.; MELO, J. A. T.; PRATES, M. V.; PESQUERO, J. B.; SANTOS, E. L.; NAKAIE, C. R.; COSTA NETO, C. M.; BLOCH JUNIOR, C. Bradykinin-related peptides from Phyllomedusa hypochondrialis. Peptides, v. 27, 2006. Article in press.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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4. | | ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. Plos One, v. 10, dez. 2015. (Open Access)Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 4 | |
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