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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
17/12/2013 |
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Data da última atualização: |
17/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COSTA, S. R. da; SANTOS, C. A. F. |
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Afiliação: |
S. R. da COSTA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; CARLOS ANTONIO FERNANDES SANTOS, CPATSA. |
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Título: |
Allelic database and divergence among Psidium accessions by using microsatellite markers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 4, p. 6802-6812, 2013. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species. MenosThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variabili... Mostrar Tudo |
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Palavras-Chave: |
Banco ativo de germoplasma; Dendrograma; Guava; Marcadores microssatélites; Melhoramento genético; Variabilidade genética. |
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Thesagro: |
Goiaba. |
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Thesaurus Nal: |
Breeding; Psidium. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94165/1/Carlos-2.pdf
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Marc: |
LEADER 02480naa a2200253 a 4500
001 1974120
005 2013-12-17
008 2013 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aCOSTA, S. R. da
245 $aAllelic database and divergence among Psidium accessions by using microsatellite markers.$h[electronic resource]
260 $c2013
520 $aThis study aimed to investigate the genetic variability among guava accessions and wild Psidium species of the Embrapa Semiárido germplasm collection by using microsatellite loci to guide genetic resources and breeding programs, emphasizing crosses between guava and other Psidium species. DNA was extracted using the 2X CTAB method, and polymerase chain reaction products were analyzed on 6% denatured polyacrylamide gels stained with silver nitrate. The unweighted pair-group method using arithmetic average dendrogram generated from the distance matrix of the Jaccard coefficient for 183 alleles of 13 microsatellite loci was used for visualization of genetic similarity. The number of base pairs was estimated using inverse mobility method based on the regression of known-size products. Analysis of molecular variance was performed using total decomposition between and within guava accessions. The accessions showed similarity from 0.75 to 1.00, with the dendrogram presenting cophenetic value of 0.85. Five groups were observed: the first included guava accessions; the second, P. guineense accessions; the third, one accession of P. friedrichsthalianum; and the last 2 groups, P. cattleianum. The genetic similarity among P. guineense and some guava accessions were above 80%, suggesting greater possibility to obtain interspecies hybrids between these 2 species. The genetic variability between the accessions was considered to be high (?ST = 0.238), indicating that guava genetic variability is not uniformly distributed among the 9 Brazilian states from where the accession were obtained. Obtaining a greater number of accessions by Brazilian states is recommended in order to have greater diversity among the species.
650 $aBreeding
650 $aPsidium
650 $aGoiaba
653 $aBanco ativo de germoplasma
653 $aDendrograma
653 $aGuava
653 $aMarcadores microssatélites
653 $aMelhoramento genético
653 $aVariabilidade genética
700 1 $aSANTOS, C. A. F.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 6802-6812, 2013.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/08/2020 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MALARD, P. F.; PEIXER, M. A. S.; GRAZIA, J. G.; BRUNEL, H. dos S. S.; FERES, L. F.; VILLARROEL, C. L.; SIQUEIRA, L. G. B.; DODE, M. A. N.; POGUE, R.; VIANA, J. H. M.; CARVALHO, J. L. |
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Afiliação: |
PATRICIA F. MALARD, UCB; MAURICIO A. S. PEIXER, UCB; JOAO G. GRAZIA, CENATTE EMBRIÕES; HILANA DOS SANTOS SENA BRUNEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ F. FERES, UNIFAL; CARLA L. VILLARROEL, BIO BIOTECNOLOGIA DA REPRODUÇÃO ANIMAL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; ROBERT POGUE, UCB; JOAO HENRIQUE MOREIRA VIANA, Cenargen; JULIANA L. CARVALHO, UCB. |
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Título: |
Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, article 8018, 2020. |
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DOI: |
https://doi.org/10.1038/s41598-020-64810-x |
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Idioma: |
Inglês |
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Conteúdo: |
Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions. |
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Palavras-Chave: |
Fertility; Fertilização in-vitro; Mesenchymal stem cells; MSC; OPU; Ovum pick-up. |
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Thesagro: |
Biotecnologia; Bovino; Fertilidade Animal. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224978/1/Intraovarian-injection.pdf
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Marc: |
LEADER 02359naa a2200361 a 4500
001 2133327
005 2021-08-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-020-64810-x$2DOI
100 1 $aMALARD, P. F.
245 $aIntraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss.$h[electronic resource]
260 $c2020
520 $aValuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPUlesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.
650 $aBiotecnologia
650 $aBovino
650 $aFertilidade Animal
653 $aFertility
653 $aFertilização in-vitro
653 $aMesenchymal stem cells
653 $aMSC
653 $aOPU
653 $aOvum pick-up
700 1 $aPEIXER, M. A. S.
700 1 $aGRAZIA, J. G.
700 1 $aBRUNEL, H. dos S. S.
700 1 $aFERES, L. F.
700 1 $aVILLARROEL, C. L.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aDODE, M. A. N.
700 1 $aPOGUE, R.
700 1 $aVIANA, J. H. M.
700 1 $aCARVALHO, J. L.
773 $tScientific Reports$gv. 10, article 8018, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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