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| Registros recuperados : 38 | |
| Registros recuperados : 38 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/11/2008 |
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Data da última atualização: |
27/11/2008 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - C |
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Autoria: |
SOARES, T. L.; SILVA, S. de O. e; COSTA, M. A. P. de C.; SANTOS-SEREJO, J. A. dos; SOUZA, A. da S.; LINO, L. S. M.; SOUZA, E. H.; NUNES, O. N. de. |
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Afiliação: |
Taliane Leila Soares, UFRB; Sebastião de Oliveira e Silva, CNPMF; Maria Angélica Pereira de Carvalho Costa, UFRB; Janay Almeida dos Santos-Serejo, CNPMF; Antônio da Silva Souza, CNPMF; Lucymeire Souza Morais Lino, UEFS; Everton Hilo de Souza, UFRB; Onildo Nunes de Jesus, ESALQ/USP. |
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Título: |
In vitro germination and viability of pollen grains of banana diploids. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 8, n. 2, p. 111-118, jun. 2008 |
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ISSN: |
1518-7853 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the influence of pH on in vitro germination and pollen grain viability of banana diploids (AA) generated by the breeding program of Embrapa Mandioca e Fruticultura Tropical. The pollen grains were inoculated in culture medium containing 15% sucrose, 0.01% H3BO3, 0.01% KNO3, 0.03% Ca(NO3)2.4H2O, 0.02% MgSO4.7H2O, solidified with 0.8% agar and pH adjusted to 5.8 or 7.0. Pollen viability was evaluated by staining with 1% acetic carmine. The germination percentages of the genotypes 9187-01 (90.0%) and M-53 (89.7%) in pH 7.0 medium were highest, while the pollen tube length of genotype 9187-01 was approximately half the size (1.79mm) of genotype M-53 (3.84mm). The pollen viability of the genotypes evaluated was higher than 85%, even for the diploids with a low in vitro germination percentage. |
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Palavras-Chave: |
Culture medium; Diploid; Genetic breeding; Pollen tube. |
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Thesagro: |
Musa Acuminata. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01631naa a2200277 a 4500
001 1636400
005 2008-11-27
008 2008 bl uuuu u00u1 u #d
022 $a1518-7853
100 1 $aSOARES, T. L.
245 $aIn vitro germination and viability of pollen grains of banana diploids.
260 $c2008
520 $aThe objective of this study was to evaluate the influence of pH on in vitro germination and pollen grain viability of banana diploids (AA) generated by the breeding program of Embrapa Mandioca e Fruticultura Tropical. The pollen grains were inoculated in culture medium containing 15% sucrose, 0.01% H3BO3, 0.01% KNO3, 0.03% Ca(NO3)2.4H2O, 0.02% MgSO4.7H2O, solidified with 0.8% agar and pH adjusted to 5.8 or 7.0. Pollen viability was evaluated by staining with 1% acetic carmine. The germination percentages of the genotypes 9187-01 (90.0%) and M-53 (89.7%) in pH 7.0 medium were highest, while the pollen tube length of genotype 9187-01 was approximately half the size (1.79mm) of genotype M-53 (3.84mm). The pollen viability of the genotypes evaluated was higher than 85%, even for the diploids with a low in vitro germination percentage.
650 $aMusa Acuminata
653 $aCulture medium
653 $aDiploid
653 $aGenetic breeding
653 $aPollen tube
700 1 $aSILVA, S. de O. e
700 1 $aCOSTA, M. A. P. de C.
700 1 $aSANTOS-SEREJO, J. A. dos
700 1 $aSOUZA, A. da S.
700 1 $aLINO, L. S. M.
700 1 $aSOUZA, E. H.
700 1 $aNUNES, O. N. de
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 8, n. 2, p. 111-118, jun. 2008
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