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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/12/2016 |
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Data da última atualização: |
08/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, R. L. da; FERREIRA, C. F.; LEDO, C. A. da S.; SOUZA, E. H. de; SILVA, P. H. da; COSTA, M. A. P. de C.; SOUZA, F. V. D. |
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Afiliação: |
RONILZE LEITE DA SILVA, UFRB; CLAUDIA FORTES FERREIRA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVERTON HILO DE SOUZA, CAPES; PAULO HENRIQUE DA SILVA, UFRB; MARIA ANGÉLICA PEREIRA DE CARVALHO COSTA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Viability and genetic stability of pineapple germplasm after 10 years of in vitro conservation. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.127, p.123-133, 2016. |
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ISSN: |
0167-6857 |
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Idioma: |
Inglês |
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Conteúdo: |
The resumed growth of plants conserved in vitro and the maintenance of genetic stability after long periods in a slow growth regime are fundamental to the success of in vitro conservation. This study aimed to evaluate the viability of pineapple plants conserved in vitro for 10 years, by triggering resumed growth and measuring the propagative potential and genetic fidelity using ISSR markers. Conserved plants of 66 accessions were micropropagated by five subcultures with an interval of 45 days. The number of shoots was counted and the propagative potential was calculated by geometric growth of the different accessions. A box plot was constructed to express the variability of the botanical varieties during the subcultures and a Poisson log-linear model was fitted to the data from multiplication of the shoots, and the genetic stability was studied with ISSR markers. All the preserved accessions resumed growing after 10 years. The geometric growth rate indicated a reduction of the propagative potential of all the accessions evaluated. No somaclonal variation was detected in the plants of the Ananas comosus var. comosus and A. comosus var. ananassoides, but probable somaclonal variants were detected in two accessions of A. comosus var. bracteatus, when compared to the stock plant in the field. The results allow standardizing an interval of 24 months between subcultures in the accessions evaluated, facilitating management of the bank. This is the first report of the effect of in vitro conservation on the resumed growth, propagative potential and genetic stability of pineapple plants. MenosThe resumed growth of plants conserved in vitro and the maintenance of genetic stability after long periods in a slow growth regime are fundamental to the success of in vitro conservation. This study aimed to evaluate the viability of pineapple plants conserved in vitro for 10 years, by triggering resumed growth and measuring the propagative potential and genetic fidelity using ISSR markers. Conserved plants of 66 accessions were micropropagated by five subcultures with an interval of 45 days. The number of shoots was counted and the propagative potential was calculated by geometric growth of the different accessions. A box plot was constructed to express the variability of the botanical varieties during the subcultures and a Poisson log-linear model was fitted to the data from multiplication of the shoots, and the genetic stability was studied with ISSR markers. All the preserved accessions resumed growing after 10 years. The geometric growth rate indicated a reduction of the propagative potential of all the accessions evaluated. No somaclonal variation was detected in the plants of the Ananas comosus var. comosus and A. comosus var. ananassoides, but probable somaclonal variants were detected in two accessions of A. comosus var. bracteatus, when compared to the stock plant in the field. The results allow standardizing an interval of 24 months between subcultures in the accessions evaluated, facilitating management of the bank. This is the first report of the effect of in v... Mostrar Tudo |
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Palavras-Chave: |
Pineapple. |
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Thesagro: |
Abacaxi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02285naa a2200229 a 4500
001 2059255
005 2023-05-08
008 2016 bl uuuu u00u1 u #d
022 $a0167-6857
100 1 $aSILVA, R. L. da
245 $aViability and genetic stability of pineapple germplasm after 10 years of in vitro conservation.$h[electronic resource]
260 $c2016
520 $aThe resumed growth of plants conserved in vitro and the maintenance of genetic stability after long periods in a slow growth regime are fundamental to the success of in vitro conservation. This study aimed to evaluate the viability of pineapple plants conserved in vitro for 10 years, by triggering resumed growth and measuring the propagative potential and genetic fidelity using ISSR markers. Conserved plants of 66 accessions were micropropagated by five subcultures with an interval of 45 days. The number of shoots was counted and the propagative potential was calculated by geometric growth of the different accessions. A box plot was constructed to express the variability of the botanical varieties during the subcultures and a Poisson log-linear model was fitted to the data from multiplication of the shoots, and the genetic stability was studied with ISSR markers. All the preserved accessions resumed growing after 10 years. The geometric growth rate indicated a reduction of the propagative potential of all the accessions evaluated. No somaclonal variation was detected in the plants of the Ananas comosus var. comosus and A. comosus var. ananassoides, but probable somaclonal variants were detected in two accessions of A. comosus var. bracteatus, when compared to the stock plant in the field. The results allow standardizing an interval of 24 months between subcultures in the accessions evaluated, facilitating management of the bank. This is the first report of the effect of in vitro conservation on the resumed growth, propagative potential and genetic stability of pineapple plants.
650 $aAbacaxi
653 $aPineapple
700 1 $aFERREIRA, C. F.
700 1 $aLEDO, C. A. da S.
700 1 $aSOUZA, E. H. de
700 1 $aSILVA, P. H. da
700 1 $aCOSTA, M. A. P. de C.
700 1 $aSOUZA, F. V. D.
773 $tPlant Cell, Tissue and Organ Culture$gv.127, p.123-133, 2016.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
14/01/2009 |
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Data da última atualização: |
12/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
ARAÚJO, R. S.; PROCÓPIO, S. de O.; SANTOS, J. B. dos; PIRES, F. R.; SILVA, A. A. da; SANTOS, E. A. dos; CARGNELUTTI FILHO, A.; RIBEIRO JÚNIOR, J. I. |
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Afiliação: |
Rodrigo Silva Araújo, UFES; Sérgio de Oliveira Procópio, Embrapa Tabuleiros Costeiros; José Barbosa dos Santos, UNIVALE; Fábio Ribeiro Pires, UFES; Antônio Alberto da Silva, UFV; Edson Aparecido dos Santos, UFV; Alberto Cargnelutti Filho, UFRGS; José Ivo Ribeiro Júnior, UFV. |
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Título: |
Produtividade de feijão em solo contaminado com o herbicida Trifloxysulfuron Sodium, após o fitorremediaçãopor mucunã-preta e feijão-de-porco. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÕ DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Anais... Rio de Janeiro: Sociedade Brasileira de Ciência do Solo, 2008. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Descontaminação. |
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Thesagro: |
Adubo Verde; Contaminação; Solo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00890naa a2200241 a 4500
001 1372364
005 2009-02-12
008 2008 bl uuuu u00u1 u #d
100 1 $aARAÚJO, R. S.
245 $aProdutividade de feijão em solo contaminado com o herbicida Trifloxysulfuron Sodium, após o fitorremediaçãopor mucunã-preta e feijão-de-porco.
260 $c2008
650 $aAdubo Verde
650 $aContaminação
650 $aSolo
653 $aDescontaminação
700 1 $aPROCÓPIO, S. de O.
700 1 $aSANTOS, J. B. dos
700 1 $aPIRES, F. R.
700 1 $aSILVA, A. A. da
700 1 $aSANTOS, E. A. dos
700 1 $aCARGNELUTTI FILHO, A.
700 1 $aRIBEIRO JÚNIOR, J. I.
773 $tIn: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÕ DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Anais... Rio de Janeiro: Sociedade Brasileira de Ciência do Solo, 2008. 1 CD-ROM.
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Embrapa Tabuleiros Costeiros (CPATC) |
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