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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
18/02/2010 |
Data da última atualização: |
16/08/2010 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
COSTA, J. G. C. da. |
Afiliação: |
JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF. |
Título: |
Conhecendo a planta do feijoeiro comum. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: KLUTHCOUSKI, J.; STONE, L. F.; AIDAR, H. (Ed.). Fundamentos para uma agricultura sustentável, com ênfase na cultura do feijoeiro. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2009. |
Páginas: |
p. 35-43. |
ISBN: |
978-85-7437-033-0 |
Idioma: |
Português |
Conteúdo: |
A planta de feijão. A plasticidade. |
Thesagro: |
Feijão; Melhoramento genético vegetal; Phaseolus vulgaris. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 00662naa a2200181 a 4500 001 1658101 005 2010-08-16 008 2009 bl uuuu u00u1 u #d 020 $a978-85-7437-033-0 100 1 $aCOSTA, J. G. C. da 245 $aConhecendo a planta do feijoeiro comum. 260 $c2009 300 $ap. 35-43. 520 $aA planta de feijão. A plasticidade. 650 $aFeijão 650 $aMelhoramento genético vegetal 650 $aPhaseolus vulgaris 773 $tIn: KLUTHCOUSKI, J.; STONE, L. F.; AIDAR, H. (Ed.). Fundamentos para uma agricultura sustentável, com ênfase na cultura do feijoeiro. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2009.
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Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
11/12/2019 |
Data da última atualização: |
11/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
Afiliação: |
Lucas J. L. Pizauro, UNESP; Camila C. de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Fernando A. de Ávila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
Título: |
Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Journal of Dairy Science, v.102, n.12, dec. 2019. |
Páginas: |
p.11459-11464 |
DOI: |
https://doi.org/10.3168/jds.2018-15920 |
Idioma: |
Inglês |
Notas: |
Short communication. |
Conteúdo: |
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. MenosThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also dete... Mostrar Tudo |
Palavras-Chave: |
Dairy buffalo; Virulence genes. |
Thesaurus NAL: |
Antibiotic resistance; Biofilm. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02937naa a2200277 a 4500 001 2116604 005 2019-12-11 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3168/jds.2018-15920$2DOI 100 1 $aPIZAURO, L. J. L. 245 $aDetection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.$h[electronic resource] 260 $c2019 300 $ap.11459-11464 500 $aShort communication. 520 $aThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. 650 $aAntibiotic resistance 650 $aBiofilm 653 $aDairy buffalo 653 $aVirulence genes 700 1 $aALMEIDA, C. C. de 700 1 $aSOLTES, G. A. 700 1 $aSLAVIC, D. 700 1 $aÁVILA, F. A. de 700 1 $aZAFALON, L. F. 700 1 $aMACINNES, J. I. 773 $tJournal of Dairy Science$gv.102, n.12, dec. 2019.
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