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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
12/06/2020 |
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Data da última atualização: |
12/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, S. da S.; COSTA, J. F. da V.; BARROS, B. de A.; CARNEIRO, A. A.; SOUSA, S. M. de. |
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Afiliação: |
Simara da Silva Lopes, Universidade Federal de São João del-Rei; Jéssica Fabiane da Veiga Costa, Centro Universitário de Sete Lagoas; BEATRIZ DE ALMEIDA BARROS, CNPMS; ANDREA ALMEIDA CARNEIRO, CNPMS; SYLVIA MORAIS DE SOUSA TINOCO, CNPMS. |
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Título: |
Auxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 20, n. 2, e289220212, 2020. |
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DOI: |
http://dx.doi.org/10.1590/1984- 70332020v20n2a28 |
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Idioma: |
Inglês |
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Conteúdo: |
Determining the copy number is important because it can greatly influence the expression level and genetic stability of transgenes. This study aimed to establish a methodology that can estimate the transgene copy number in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as an endogenous control. Tobacco c.v. Petit Havana plants were transformed via Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a selective gene. Oligonucleotide efficiency was determined and a qPCR using the 2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was the endogenous control in five transgenic tobacco events. The results showed that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific hybridization occurred between N. sylvestris and N. tomentosiformis. The copy number results agreed with the segregation ratios for the Bar gene in T1 plants, which confirmed that Axi1 is a single copy gene that can be used as an endogenous control. |
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Palavras-Chave: |
Controle endógeno; Cópia transgênica; Tabaco. |
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Thesagro: |
Gene; Nicotiana Tabacum; Planta Transgênica. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/213949/1/Auxin-independent.pdf
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Marc: |
LEADER 01836naa a2200253 a 4500
001 2123250
005 2020-06-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/1984- 70332020v20n2a28$2DOI
100 1 $aLOPES, S. da S.
245 $aAuxin independent1 (Axi1) as an endogenous gene for copy number determination in transgenic tobacco.$h[electronic resource]
260 $c2020
520 $aDetermining the copy number is important because it can greatly influence the expression level and genetic stability of transgenes. This study aimed to establish a methodology that can estimate the transgene copy number in tobacco (Nicotiana tabacum) using the Auxin independent1 (Axi1) gene as an endogenous control. Tobacco c.v. Petit Havana plants were transformed via Agrobacterium tumefaciens with a pMCG1005 vector that contained Bar as a selective gene. Oligonucleotide efficiency was determined and a qPCR using the 2-ΔCt and 2-ΔΔCt methods was performed. Bar was the target gene and Axi1 was the endogenous control in five transgenic tobacco events. The results showed that the Axi1 gene was donated by the maternal parent N. sylvestris when interspecific hybridization occurred between N. sylvestris and N. tomentosiformis. The copy number results agreed with the segregation ratios for the Bar gene in T1 plants, which confirmed that Axi1 is a single copy gene that can be used as an endogenous control.
650 $aGene
650 $aNicotiana Tabacum
650 $aPlanta Transgênica
653 $aControle endógeno
653 $aCópia transgênica
653 $aTabaco
700 1 $aCOSTA, J. F. da V.
700 1 $aBARROS, B. de A.
700 1 $aCARNEIRO, A. A.
700 1 $aSOUSA, S. M. de
773 $tCrop Breeding and Applied Biotechnology$gv. 20, n. 2, e289220212, 2020.
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Embrapa Milho e Sorgo (CNPMS) |
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