Data/hora: 24/06/2024 18:42:24

Biblioteca(s): | Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 11/12/2020
Data da última atualização: | 11/12/2020
Autoria: | MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L.
Afiliação: | CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen.
Título: | Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.
Ano de publicação: | 2021
Fonte/Imprenta: | Theriogenology, Vv. 160, p. 134-141, 2021.
Páginas: | p. 134-141
ISSN: | 0093-691X
Idioma: | Português
Conteúdo: | In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
Palavras-Chave: | Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação.
Thesagro: | Cultura de Embrião; Embrião.
Categoria do assunto: | --
Registro original: | Embrapa Cerrados (CPAC)

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CPAC - (36842-1) - UPC - AP - DD - DIGITAL -  - DIGITAL - DIS
CENARGEN - (38471-1) - UPC - AP - DD -  -  -  - DIS