Data/hora: 19/06/2024 10:36:19

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 17/11/2021
Data da última atualização: | 17/11/2021
Autoria: | SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z.
Afiliação: | JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; DANIEL ANDREWS DE MOURA FERNANDES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; VIVIANE LOPES BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ANA BEATRIZ DA SILVA CARVALHO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.
Título: | Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.
Ano de publicação: | 2021
Fonte/Imprenta: | Veterinary Record, e1064, 2021.
DOI: | https://doi.org/10.1002/vetr.1064
Idioma: | Inglês
Conteúdo: | Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
Thesaurus Nal: | Sheep.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (39892-1) - UPC - AP - DD -  -  -  - DIS