| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Florestas. |
|
Data corrente: |
20/12/2019 |
|
Data da última atualização: |
20/12/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
IARK, D.; BUZZO, A. J. dos R.; GARCIA, J. A. A.; CORREA, V. G.; HELM, C. V.; CORRÊA, R. C. G.; PERALTA, R. A.; MOREIRA, R. de F. P. M.; BRACHT, A.; PERALTA, R. M. |
|
Afiliação: |
Daiane Iark, UEM; Ana Júlia dos Reis Buzzo, UEM; Jéssica Amanda Andrade Garcia, UEM; Vanesa Gesser Correa, UEM; CRISTIANE VIEIRA HELM, CNPF; Rúbia Carvalho Gomes Corrêa, UEM; Rosely A. Peralta, UFSC; Regina de Fátima Peralta Muniz Moreira, UFSC; Adelar Bracht, UEM; Rosane Marina Peralta, UEM. |
|
Título: |
Enzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Bioresource Technology, 289, article 121655, Oct. 2019. 7 p. |
|
DOI: |
10.1016/j.biortech.2019.121655 |
|
Idioma: |
Inglês |
|
Conteúdo: |
A single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. |
|
Palavras-Chave: |
Biorremediação; Dye degradation; Lacase. |
|
Thesaurus Nal: |
Azo dyes; Laccase; White-rot fungi. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 01891naa a2200313 a 4500
001 2117496
005 2019-12-20
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biortech.2019.121655$2DOI
100 1 $aIARK, D.
245 $aEnzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii.$h[electronic resource]
260 $c2019
520 $aA single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes.
650 $aAzo dyes
650 $aLaccase
650 $aWhite-rot fungi
653 $aBiorremediação
653 $aDye degradation
653 $aLacase
700 1 $aBUZZO, A. J. dos R.
700 1 $aGARCIA, J. A. A.
700 1 $aCORREA, V. G.
700 1 $aHELM, C. V.
700 1 $aCORRÊA, R. C. G.
700 1 $aPERALTA, R. A.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
773 $tBioresource Technology, 289, article 121655, Oct. 2019. 7 p.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Florestas (CNPF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
05/02/2014 |
|
Data da última atualização: |
06/02/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
MONTEIRO, F. M.; MERCADANTE, M. E.; BARROS, C. M.; SATRAPA, R. A.; SILVA, J. A.; OLIVEIRA, L. Z.; SARAIVA, N. Z.; OLIVEIRA, C. S.; GARCIA, J. M. |
|
Afiliação: |
F. M. MONTEIRO, APTA / UNESP; M. E. MERCADANTE, APTA; C. M. BARROS, UNESP; R. A. SATRAPA, UNESP; J. A. SILVA, UNESP; L. Z. OLIVEIRA, UNESP; N. Z. SARAIVA, UNESP; CLARA SLADE OLIVEIRA, CNPGL; J. M. GARCIA, UNESP. |
|
Título: |
Reproductive tract development and puberty in two lines of Nellore heifers selected for postweaning weight. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Theriogenology, v. 80, n. 1, p. 10-17, 2013. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2013.02.013 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective was to evaluate reproductive tract development (ovary and uterus) and onset of puberty in two lines of Nellore heifers (Bos indicus) selected for postweaning weight. A total of 123 heifers, including 46 from the control Nellore line (NeC) and 77 from the selection Nellore line (NeS) were used. Every 18 to 21 days from 12 to 24 months of age, average ovarian area (OVA), endometrial thickness (ETh), and diameter of the largest follicle in each ovary were evaluated (using transrectal ultrasonography), and body weight, hip height, and body condition score were measured. There were no differences between NeS and NeC heifers for ETh or OVA (P < 0.05). Genetic selection for higher postweaning weight had no negative influence on the onset of puberty, with 52% and 48% of NeC and NeS heifers, respectively, pubertal at 24 months of age (P = 0.49). Heifers that reached puberty at the end of the study were heavier (NeC, 296.9 vs. 276.7 kg; NeS, 343.5 vs. 327.9 kg; P < 0.01) and younger (NeC, 23.4 vs. 24.2 mo; NeS, 22.7 vs. 24.0 months; P < 0.01) than those that did not. Furthermore, heifers that were heavier at weaning reached puberty earlier. Pubertal heifers had a greater OVA (4.15 vs. 3.14 cm(2); P < 0.01) and ETh (12.15 vs. 9.93 mm; P < 0.01) than nonpubertal heifers. Taken together, OVA and ETh had positive effects (P < 0.01) on the onset of puberty and were suitable indicator traits of heifer sexual precocity in pasture management systems. However, selection for weight did not alter ovarian or endometrial development, or manifestation of puberty at 24 months of age. Among the growth traits studied, weaning weight and weight at puberty had significant positive effects on manifestation of first estrus. MenosThe objective was to evaluate reproductive tract development (ovary and uterus) and onset of puberty in two lines of Nellore heifers (Bos indicus) selected for postweaning weight. A total of 123 heifers, including 46 from the control Nellore line (NeC) and 77 from the selection Nellore line (NeS) were used. Every 18 to 21 days from 12 to 24 months of age, average ovarian area (OVA), endometrial thickness (ETh), and diameter of the largest follicle in each ovary were evaluated (using transrectal ultrasonography), and body weight, hip height, and body condition score were measured. There were no differences between NeS and NeC heifers for ETh or OVA (P < 0.05). Genetic selection for higher postweaning weight had no negative influence on the onset of puberty, with 52% and 48% of NeC and NeS heifers, respectively, pubertal at 24 months of age (P = 0.49). Heifers that reached puberty at the end of the study were heavier (NeC, 296.9 vs. 276.7 kg; NeS, 343.5 vs. 327.9 kg; P < 0.01) and younger (NeC, 23.4 vs. 24.2 mo; NeS, 22.7 vs. 24.0 months; P < 0.01) than those that did not. Furthermore, heifers that were heavier at weaning reached puberty earlier. Pubertal heifers had a greater OVA (4.15 vs. 3.14 cm(2); P < 0.01) and ETh (12.15 vs. 9.93 mm; P < 0.01) than nonpubertal heifers. Taken together, OVA and ETh had positive effects (P < 0.01) on the onset of puberty and were suitable indicator traits of heifer sexual precocity in pasture management systems. However, selection for weigh... Mostrar Tudo |
|
Palavras-Chave: |
Beef heifers; Endometrial thickness; Ovary; Ultrasound. |
|
Thesagro: |
Bos Indicus. |
|
Thesaurus NAL: |
puberty. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02639naa a2200301 a 4500
001 1978738
005 2024-02-06
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2013.02.013$2DOI
100 1 $aMONTEIRO, F. M.
245 $aReproductive tract development and puberty in two lines of Nellore heifers selected for postweaning weight.$h[electronic resource]
260 $c2013
520 $aThe objective was to evaluate reproductive tract development (ovary and uterus) and onset of puberty in two lines of Nellore heifers (Bos indicus) selected for postweaning weight. A total of 123 heifers, including 46 from the control Nellore line (NeC) and 77 from the selection Nellore line (NeS) were used. Every 18 to 21 days from 12 to 24 months of age, average ovarian area (OVA), endometrial thickness (ETh), and diameter of the largest follicle in each ovary were evaluated (using transrectal ultrasonography), and body weight, hip height, and body condition score were measured. There were no differences between NeS and NeC heifers for ETh or OVA (P < 0.05). Genetic selection for higher postweaning weight had no negative influence on the onset of puberty, with 52% and 48% of NeC and NeS heifers, respectively, pubertal at 24 months of age (P = 0.49). Heifers that reached puberty at the end of the study were heavier (NeC, 296.9 vs. 276.7 kg; NeS, 343.5 vs. 327.9 kg; P < 0.01) and younger (NeC, 23.4 vs. 24.2 mo; NeS, 22.7 vs. 24.0 months; P < 0.01) than those that did not. Furthermore, heifers that were heavier at weaning reached puberty earlier. Pubertal heifers had a greater OVA (4.15 vs. 3.14 cm(2); P < 0.01) and ETh (12.15 vs. 9.93 mm; P < 0.01) than nonpubertal heifers. Taken together, OVA and ETh had positive effects (P < 0.01) on the onset of puberty and were suitable indicator traits of heifer sexual precocity in pasture management systems. However, selection for weight did not alter ovarian or endometrial development, or manifestation of puberty at 24 months of age. Among the growth traits studied, weaning weight and weight at puberty had significant positive effects on manifestation of first estrus.
650 $apuberty
650 $aBos Indicus
653 $aBeef heifers
653 $aEndometrial thickness
653 $aOvary
653 $aUltrasound
700 1 $aMERCADANTE, M. E.
700 1 $aBARROS, C. M.
700 1 $aSATRAPA, R. A.
700 1 $aSILVA, J. A.
700 1 $aOLIVEIRA, L. Z.
700 1 $aSARAIVA, N. Z.
700 1 $aOLIVEIRA, C. S.
700 1 $aGARCIA, J. M.
773 $tTheriogenology$gv. 80, n. 1, p. 10-17, 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
