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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
CORRÊA, L. B. N. S.; CARDOZO, L. M. de F.; RIBEIRO, I. C. de A.; BOAVENTURA, G. T.; CHAGAS, M. A. |
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Afiliação: |
Lanna B. N. S. Corrêa, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF; Ludmila F. M. de F. Cardozo, Laboratório de Nutrição Experimental - LabNE/Departamento de Nutrição/Universidade Federal Fluminense - UFF; Ilma C. de A. Ribeiro, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF; Gilson T. Boaventura, Laboratório de Nutrição Experimental - LabNE/Departamento de Nutrição/Universidade Federal Fluminense - UFF; Maurício A. Chagas, Laboratório de Biomorfologia Celular e Extracelular/Departamento de Morfologia/Universidade Federal Fluminense - UFF. |
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Título: |
Influence of prolonged flaxseed (Linum usitatissimum) consumption over epididymis and testicle histoarchitecture of Wistar rats. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 650-656, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Influência do consumo prolongado de semente de linhaça (Linum usitatissimum) sobre a histoarquitetura dos testículo e epidídimo de ratos Wistar. |
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Conteúdo: |
Flaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantification showed no significant differences in the FG (P=0.98). These results revealed that the continuous and prolonged intake of 25% flaxseed meals from gestation to 250 days of age, even with a significant increase in serum levels of estradiol, does not exert adverse effects on the testicular and epididymal structure or on the cells participating in the spermatogenesis of rats. MenosFlaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantifica... Mostrar Tudo |
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Palavras-Chave: |
Células de Sertoli; Fitoestrógenos; Histomorfometria; Histomorphometry; Ratos Wistar; Túbulos seminíferos; Vimentina. |
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Thesagro: |
Linhaça; Linum Usitatissimum; Semente; Testículo. |
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Thesaurus Nal: |
Epididymis; Linseed; Plant estrogens; Seminiferous tubules; Sertoli cells; Testes; Vimentin. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163970/1/Influence-of-prolonged-flaxseed.pdf
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Marc: |
LEADER 03207naa a2200397 a 4500
001 2075874
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aCORRÊA, L. B. N. S.
245 $aInfluence of prolonged flaxseed (Linum usitatissimum) consumption over epididymis and testicle histoarchitecture of Wistar rats.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Influência do consumo prolongado de semente de linhaça (Linum usitatissimum) sobre a histoarquitetura dos testículo e epidídimo de ratos Wistar.
520 $aFlaxseed is considered a functional food with several health benefits. However, because of its high phytoestrogen content, flaxseed influences hormone metabolism and affects the gonadal biomorphology. In this study, computerized histomorphometry was used to evaluate seminiferous and epididymal tubules, considering the different regions of the epididymis (head, body and tail) of rats subjected to a prolonged diet of flaxseed. Young adult male Wistar rats (n=20) were divided into 2 groups during their lactation period: Control Group (CG), fed casein-based meals and Flaxseed Group (FG), fed a 25% flaxseed meal. After 250 days of continuous ingestion, the animals were euthanized and a blood sample was collected. The testicles and epididymis were removed and fixed in buffered formalin solution. The samples were subjected to routine histological paraffin techniques and stained with hematoxilin and eosin. Immunostaining was performed using an antivimentin antibody for Sertoli cell identification. For morphometry, images of the slides were scanned and analyzed using Image J to determine the epithelial height, tubular and luminal diameter and tubular and luminal area. In the hormonal evaluation, FG had a higher serum concentration of estrogen (P=0.001), but no change was observed in the concentration of testosterone. The morphometric assay of seminiferous tubules and epididymal regions revealed no significant differences between the analyzed groups. Similarly, Sertoli cell quantification showed no significant differences in the FG (P=0.98). These results revealed that the continuous and prolonged intake of 25% flaxseed meals from gestation to 250 days of age, even with a significant increase in serum levels of estradiol, does not exert adverse effects on the testicular and epididymal structure or on the cells participating in the spermatogenesis of rats.
650 $aEpididymis
650 $aLinseed
650 $aPlant estrogens
650 $aSeminiferous tubules
650 $aSertoli cells
650 $aTestes
650 $aVimentin
650 $aLinhaça
650 $aLinum Usitatissimum
650 $aSemente
650 $aTestículo
653 $aCélulas de Sertoli
653 $aFitoestrógenos
653 $aHistomorfometria
653 $aHistomorphometry
653 $aRatos Wistar
653 $aTúbulos seminíferos
653 $aVimentina
700 1 $aCARDOZO, L. M. de F.
700 1 $aRIBEIRO, I. C. de A.
700 1 $aBOAVENTURA, G. T.
700 1 $aCHAGAS, M. A.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 650-656, jun. 2017.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Semiárido; Embrapa Unidades Centrais. |
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Data corrente: |
28/02/2013 |
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Data da última atualização: |
24/07/2017 |
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Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
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Autoria: |
CALGARO, M.; BRAGA, M. B. (ed.). |
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Afiliação: |
MARCELO CALGARO, CPATSA; MARCOS BRANDAO BRAGA, CNPH. |
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Título: |
A cultura da acerola. |
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Edição: |
3. ed. rev. e ampl. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Brasília, DF: Embrapa, 2012. |
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Páginas: |
144 p. |
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Descrição Física: |
il. color. |
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Série: |
(Coleção plantar, 69). |
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ISBN: |
978-85-7035-130-2 |
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Idioma: |
Português |
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Notas: |
Projeto Minibibliotecas. |
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Conteúdo: |
Introdução. Descrição botânica e variedades. Clima. Adubação e calagem. Preparo do solo. Propagação e preparo de mudas. Plantio. Podas e raleio. Irrigação. Consorciação. Controle de invasoras. Pragas da aceroleira. Controle de doenças. Colheita e pós-colheita. Coeficientes de produção e rentabilidade da exploração. |
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Palavras-Chave: |
Cereja-das-Antilhas; Consorciação; Controle de doenças - acerola; Cultivo; Plantio - acerola; Pragas - acerola; Solo - acerola; West indian cherry. |
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Thesagro: |
Acerola; Colheita; Fruticultura; Irrigação; Malpighia Glabra; Plantio; Poda; Pós-Colheita; Prática cultural; Variedade. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/128278/1/PLANTAR-Acerola-ed03-2012.pdf
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Marc: |
LEADER 01346nam a2200397 a 4500
001 1952709
005 2017-07-24
008 2012 bl uuuu 00u1 u #d
020 $a978-85-7035-130-2
100 1 $aCALGARO, M.
245 $aA cultura da acerola.
250 $a3. ed. rev. e ampl.
260 $aBrasília, DF: Embrapa$c2012
300 $a144 p.$cil. color.
490 $a(Coleção plantar, 69).
500 $aProjeto Minibibliotecas.
520 $aIntrodução. Descrição botânica e variedades. Clima. Adubação e calagem. Preparo do solo. Propagação e preparo de mudas. Plantio. Podas e raleio. Irrigação. Consorciação. Controle de invasoras. Pragas da aceroleira. Controle de doenças. Colheita e pós-colheita. Coeficientes de produção e rentabilidade da exploração.
650 $aAcerola
650 $aColheita
650 $aFruticultura
650 $aIrrigação
650 $aMalpighia Glabra
650 $aPlantio
650 $aPoda
650 $aPós-Colheita
650 $aPrática cultural
650 $aVariedade
653 $aCereja-das-Antilhas
653 $aConsorciação
653 $aControle de doenças - acerola
653 $aCultivo
653 $aPlantio - acerola
653 $aPragas - acerola
653 $aSolo - acerola
653 $aWest indian cherry
700 1 $aBRAGA, M. B.
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