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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/05/2014 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. |
Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
Título: |
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Toxins, v. 6, p. 1598-1614, 2014. |
DOI: |
10.3390/toxins6051598 |
Idioma: |
Inglês |
Conteúdo: |
Bioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. MenosBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activ... Mostrar Tudo |
Thesagro: |
Toxina. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02484naa a2200217 a 4500 001 1987282 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 024 7 $a10.3390/toxins6051598$2DOI 100 1 $aARGÔLO FILHO, R. C. 245 $aRequirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.$h[electronic resource] 260 $c2014 520 $aBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. 650 $aToxina 700 1 $aCOSTA, R. L. 700 1 $aPINHEIRO, H. E. 700 1 $aCORRÊA, F. M. 700 1 $aVALICENTE, F. H. 700 1 $aPOMELLA, A. W. V. 700 1 $aLOGUERCIO, L. L. 773 $tToxins$gv. 6, p. 1598-1614, 2014.
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Embrapa Milho e Sorgo (CNPMS) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
22/11/2016 |
Data da última atualização: |
18/10/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BECARO, A. A.; PUTI, F. C.; PANOSSO, A. R.; GERN, J. C.; BRANDÃO, H. M.; CORREA, D. S.; FERREIRA, M. D. |
Afiliação: |
DANIEL SOUZA CORREA, CNPDIA; MARCOS DAVID FERREIRA, CNPDIA. |
Título: |
Postharvest quality of fresh-cut carrots packaged in plastic films containing silver nanoparticles. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Food and bioprocess technology, v. 9, p. 637-649, 2016. |
Idioma: |
Inglês |
Palavras-Chave: |
Alimentos; Pos colheita. |
Thesagro: |
Embalagem; Qualidade. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00649naa a2200229 a 4500 001 2056791 005 2017-10-18 008 2016 bl --- 0-- u #d 100 1 $aBECARO, A. A. 245 $aPostharvest quality of fresh-cut carrots packaged in plastic films containing silver nanoparticles. 260 $c2016 650 $aEmbalagem 650 $aQualidade 653 $aAlimentos 653 $aPos colheita 700 1 $aPUTI, F. C. 700 1 $aPANOSSO, A. R. 700 1 $aGERN, J. C. 700 1 $aBRANDÃO, H. M. 700 1 $aCORREA, D. S. 700 1 $aFERREIRA, M. D. 773 $tFood and bioprocess technology$gv. 9, p. 637-649, 2016.
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