Data/hora: 05/06/2024 13:14:19

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 05/10/1995
Data da última atualização: | 05/10/1995
Autoria: | CASTRIC, P. A.; CONN, E. E.
Afiliação: | Department of Biochemistry and Biophysics, University of California, Davis, California.
Título: | Formation of b-cyanoalanine by o-acetylserine sulfhydrylase.
Ano de publicação: | 1971
Fonte/Imprenta: | Journal of Bacteriology, v.108, n.1, p.132-136, 1971.
Idioma: | Inglês
Conteúdo: | Cell-free extracts of bacillus megaterium form B-cyanolanine (B-CNA)-14C from Na14CN and L-cysteine, O-acetyl-serine or, to a lesser extent, L-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of B-CNA from cysteine and cyanide. The formation of B-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or L-djenkolic acid as souces: such cells also exhibit an increased ability to from cysteine when compared with cells grown on cysteine as the sulfur source. B-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification on the O-acetylsering sulfhydrylase resulted in the co-purification of the B-CNA- forming activity.
Palavras-Chave: | HCN; Purificacao de enzinas.
Thesagro: | Purificação.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (16343-1) - ADC - SP - -- - S197 -  - S297 - DIS