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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
19/08/2019 |
Data da última atualização: |
19/08/2019 |
Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
Autoria: |
HANASHIRO, M. M.; CLEMENTE, F. M. V. T.; LOURENÇO JUNIOR, V.; BUENO, M. F. |
Afiliação: |
MARCELO MIKIO HANASHIRO, CNPH; FLAVIA MARIA VIEIRA TEIXEIRA CLEMEN, CNPH; VALDIR LOURENCO JUNIOR, CNPH; MURILO FELIPE BUENO, CNPH. |
Título: |
Avaliação de impactos ex-ante do projeto de pesquisa de controle da podridão branca em alho e cebola no Brasil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Brasília, DF: Embrapa, 2019. |
Páginas: |
34 p. |
Série: |
(Embrapa Hortaliças. Boletim de pesquisa e desenvolvimento, 182). |
ISSN: |
1677-2229 |
Idioma: |
Português |
Conteúdo: |
O principal objetivo neste trabalho foi analisar ex-ante os impactos decorrentes de projetos de manejo da podridão branca, mostrando o potencial de geração de resultados positivos nas vertentes social, econômica, ambiental, sobre o conhecimento, capacitação e político-institucional. |
Thesagro: |
Allium Cepa; Allium Sativum; Controle Biológico; Doença Fúngica; Sclerotium Cepivorum. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/201068/1/BPD-182-15-08-2019.pdf
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Marc: |
LEADER 01061nam a2200241 a 4500 001 2111442 005 2019-08-19 008 2019 bl uuuu u0uu1 u #d 022 $a1677-2229 100 1 $aHANASHIRO, M. M. 245 $aAvaliação de impactos ex-ante do projeto de pesquisa de controle da podridão branca em alho e cebola no Brasil.$h[electronic resource] 260 $aBrasília, DF: Embrapa$c2019 300 $a34 p. 490 $a(Embrapa Hortaliças. Boletim de pesquisa e desenvolvimento, 182). 520 $aO principal objetivo neste trabalho foi analisar ex-ante os impactos decorrentes de projetos de manejo da podridão branca, mostrando o potencial de geração de resultados positivos nas vertentes social, econômica, ambiental, sobre o conhecimento, capacitação e político-institucional. 650 $aAllium Cepa 650 $aAllium Sativum 650 $aControle Biológico 650 $aDoença Fúngica 650 $aSclerotium Cepivorum 700 1 $aCLEMENTE, F. M. V. T. 700 1 $aLOURENÇO JUNIOR, V. 700 1 $aBUENO, M. F.
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
23/11/2022 |
Data da última atualização: |
08/12/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
ARRAES, F. B. M.; VASQUEZ, D. D. N.; TAHIR, M.; PINHEIRO, D. H.; FAHEEM, M.; FREITAS-ALVES, N. S.; MOREIRA-PINTO, C. E.; MOREIRA, V. J. V.; PAES-DE-MELO, B.; LISEI-DE-SA, M. E.; MORGANTE, C. V.; MOTA, A. P. Z.; LOURENCO, I. T.; TOGAWA, R. C.; GRYNBERG, P.; FRAGOSO, R. da R.; ALMEIDA-ENGLER, J. de; LARSEN, M. R.; GROSSI-DE-SA, M. F. |
Afiliação: |
FABRICIO B. M. ARRAES, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; DANIEL D. N. VASQUEZ, FEDERAL UNIVERSITY OF RIO GRANDE DO SUL; MUHAMMED TAHIR, UNIVERSITY OF SOUTHERN DENMARK; DANIELE H. PINHEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; MUHAMMED FAHEEM, NATIONAL UNIVERSITY OF MEDICAL SCIENCES, PAKISTAN; NAYARA S. FREITAS-ALVES, FEDERAL UNIVERSITY OF PARANÁ; CLÍDIA E. MOREIRA-PINTO, CNPAE; VALDEIR J. V. MOREIRA, UNIVERSITY OF BRASÍLIA; BRUNO PAES-DE-MELO, CNPAE; MARIA E. LISEI-DE-SA, MINAS GERAIS AGRICULTURAL RESEARCH COMPANY; CAROLINA VIANNA MORGANTE, CPATSA; ANA P. Z. MOTA, INRAE; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; ROBERTO COITI TOGAWA, Cenargen; PRISCILA GRYNBERG, Cenargen; RODRIGO DA ROCHA FRAGOSO, CNPAE; JANICE DE ALMEIDA-ENGLER, INRAE; MARTIN R. LARSEN, UNIVERSITY OF SOUTHERN DENMARK; MARIA FATIMA GROSSI-DE-SA, Cenargen. |
Título: |
Integrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Plants, v. 11, 2744, 2022. |
ISSN: |
2223-7747 |
DOI: |
https:// doi.org/10.3390/plants11202744 |
Idioma: |
Inglês |
Conteúdo: |
The root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections. MenosThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10... Mostrar Tudo |
Palavras-Chave: |
Differential expression; Root-knot nematode. |
Thesagro: |
Glycine Max; Meloidogyne Incognita; Soja. |
Thesaurus NAL: |
Phenylpropanoids; Proteome; Transcriptome. |
Categoria do assunto: |
-- G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148619/1/Integrated-omic-approaches.pdf
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Marc: |
LEADER 03069naa a2200457 a 4500 001 2148619 005 2023-12-08 008 2022 bl uuuu u00u1 u #d 022 $a2223-7747 024 7 $ahttps:// doi.org/10.3390/plants11202744$2DOI 100 1 $aARRAES, F. B. M. 245 $aIntegrated omic approaches reveal molecular mechanisms of tolerance during soybean and meloidogyne incognita interactions.$h[electronic resource] 260 $c2022 520 $aThe root-knot nematode (RKN), Meloidogyne incognita, is a devastating soybean pathogen worldwide. The use of resistant cultivars is the most effective method to prevent economic losses caused by RKNs. To elucidate the mechanisms involved in resistance to RKN, we determined the proteome and transcriptome profiles from roots of susceptible (BRS133) and highly tolerant (PI595099) Glycine max genotypes 4, 12, and 30 days after RKN infestation. After in silico analysis, we described major defense molecules and mechanisms considered constitutive responses to nematodeinfestation, such as mTOR, PI3K-Akt, relaxin, and thermogenesis. The integrated data allowed us to identify protein families and metabolic pathways exclusively regulated in tolerant soybean genotypes. Among them, we highlighted the phenylpropanoid pathway as an early, robust, and systemic defense process capable of controlling M. incognita reproduction. Associated with this metabolic pathway, 29 differentially expressed genes encoding 11 different enzymes were identified, mainly from the flavonoid and derivative pathways. Based on differential expression in transcriptomic and proteomic data, as well as in the expression profile by RT?qPCR, and previous studies, we selected and overexpressed the GmPR10 gene in transgenic tobacco to assess its protective effect against M. incognita. Transgenic plants of the T2 generation showed up to 58% reduction in the M. incognita reproduction factor. Finally, data suggest that GmPR10 overexpression can be effective against the plant parasitic nematodeM. incognita, but its mechanism of action remains unclear. These findings will help develop new engineered soybean genotypes with higher performance in response to RKN infections. 650 $aPhenylpropanoids 650 $aProteome 650 $aTranscriptome 650 $aGlycine Max 650 $aMeloidogyne Incognita 650 $aSoja 653 $aDifferential expression 653 $aRoot-knot nematode 700 1 $aVASQUEZ, D. D. N. 700 1 $aTAHIR, M. 700 1 $aPINHEIRO, D. H. 700 1 $aFAHEEM, M. 700 1 $aFREITAS-ALVES, N. S. 700 1 $aMOREIRA-PINTO, C. E. 700 1 $aMOREIRA, V. J. V. 700 1 $aPAES-DE-MELO, B. 700 1 $aLISEI-DE-SA, M. E. 700 1 $aMORGANTE, C. V. 700 1 $aMOTA, A. P. Z. 700 1 $aLOURENCO, I. T. 700 1 $aTOGAWA, R. C. 700 1 $aGRYNBERG, P. 700 1 $aFRAGOSO, R. da R. 700 1 $aALMEIDA-ENGLER, J. de 700 1 $aLARSEN, M. R. 700 1 $aGROSSI-DE-SA, M. F. 773 $tPlants$gv. 11, 2744, 2022.
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