Data/hora: 16/06/2024 23:49:29

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 01/03/1999
Data da última atualização: | 01/03/1999
Autoria: | PIERSON, E. E.; CLARK, R. B.
Afiliação: | Department of Agronomy and U.S. Department of Agriculture, University of Nebraska Lincoln.
Título: | Ferrous iron determination in plant tissue.
Ano de publicação: | 1984
Fonte/Imprenta: | Journal of Plant Nutrition, v.7, n.1/5, p.107-116, 1984.
Idioma: | Inglês
Conteúdo: | Experiments were conducted to determine ferrous iron (iron2+, Fe2+) in plant tissues. Iron2+ could be readily extracted from leaf tissue with aqueous solutions of the chelating agents OPh (1,10-o-phenanthroline) and PDTS [ferrozine; 3-(2-pyridy1)-5,6-bis-(4-phynylsulfonic acid)-1,2,4-triazine]. Iron2+ values did not increase when leaf tissues were extracted more than 30 minutes. The first two extractions of the same tissues yielded higher Fe2+ values than subsequent extractions. After the third extraction of the same tissue, Fe2+ remained relatively constant. Even after seven extractions of the same tissue, some Fe2+ could be extracted which indicated that some iron (Fe) was probably being converted to Fe2+ with each extraction. Initial extractions of the plant material had higher Fe2+ values when extracted with OPh than PDTS, but subsequent extractions showed similar Fe2+ values for both extractants. Higher Fe2+ values were obtained from fresh leaf tossue than from freeze-dried or oven-dried tissue; freeze-dried and oven-dried tissues gave similar Fe2+ values. Total Fe measured in these tissues followed: fresh > freeze-dried > oven-dried. After the second extraction of the same tissue sample, Fe2+ extracted from each type of tissue preparation were similar.
Palavras-Chave: | Agente quelifero ferreo; Extracao de folha; Iron chelating agents; Iron nutrition; Leaf extractions; Mineral nutrition; Nutricao ferrea; Nutricao mineral.
Thesagro: | Sorghum Bicolor.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (36835-1) - ADD - SP - -- - SP3690 -  - 3690 - DIS