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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/01/2013 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CIDADE, L. C.; OLIVEIRA, T. M. de; MENDES, A. F. S.; MACEDO, A. F.; FLOH, E. I. S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. |
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Afiliação: |
LUCIANA C. CIDADE, UESC; TAHISE M. DE OLIVEIRA, UESC; AMANDA F. S. MENDES, UESC; AMANDA F. MACEDO, USP; ENY I. S. FLOH, USP; ABELMON DA SILVA GESTEIRA, CNPMF; WALTER DOS SANTOS SOARES FILHO, CNPMF; MARCIO G. C. COSTA, UESC. |
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Título: |
Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Biology Report, v.39, p. 10201-10209, 2012 |
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ISSN: |
0301-4851 |
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DOI: |
10.1007/s11033-012-1895-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The results also pave the way for further use of CpPSY, as well as other PSYs, as potential candidate genes for engineering tolerance to drought and salt stress in crop plants. MenosAbscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The... Mostrar Tudo |
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Thesaurus Nal: |
Citrus; tobacco. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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022 $a0301-4851
024 7 $a10.1007/s11033-012-1895-2$2DOI
100 1 $aCIDADE, L. C.
245 $aEctopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco.$h[electronic resource]
260 $c2012
520 $aAbscisic acid (ABA) is an important regulator of plant responses to environmental stresses and an absolute requirement for stress tolerance. Recently, a third phytoene synthase (PSY3) gene paralog was identified in monocots and demonstrated to play a specialized role in stress-induced ABA formation, thus suggesting that the first committed step in carotenogenesis is a key limiting step in ABA biosynthesis. To examine whether the ectopic expression of PSY, other than PSY3, would similarly affect ABA level and stress tolerance, we have produced transgenic tobacco containing a fruit-specific PSY (CpPSY) of grapefruit (Citrus paradisi Macf.). The transgenic plants contained a single- or double-locus insertion and expressed CpPSY at varying transcript levels. In comparison with the wild-type plants, the CpPSY expressing transgenic plants showed a significant increase on root length and shoot biomass under PEG-, NaCl- and mannitol-induced osmotic stress. The enhanced stress tolerance of transgenic plants was correlated with the increased endogenous ABA level and expression of stress-responsive genes, which in turn was correlated with the CpPSY copy number and expression level in different transgenic lines. Collectively, these results provide further evidence that PSY is a key enzyme regulating ABA biosynthesis and that the altered expression of other PSYs in transgenic plants may provide a similar function to that of the monocot?s PSY3 in ABA biosynthesis and stress tolerance. The results also pave the way for further use of CpPSY, as well as other PSYs, as potential candidate genes for engineering tolerance to drought and salt stress in crop plants.
650 $aCitrus
650 $atobacco
700 1 $aOLIVEIRA, T. M. de
700 1 $aMENDES, A. F. S.
700 1 $aMACEDO, A. F.
700 1 $aFLOH, E. I. S.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOARES FILHO, W. dos S.
700 1 $aCOSTA, M. G. C.
773 $tMolecular Biology Report$gv.39, p. 10201-10209, 2012
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 9 | |
| 1. |  | SILVA, T. E. R. da; CIDADE, L. C.; ALVIM, F. C.; CASCARDO, J. C. de M.; COSTA, M. G. C. Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 10, p. 1433-1436, out. 2008 Notas Científicas.
Título em português: Embriogênese somática e regeneração in vitro de clones elite de Theobroma cacao.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | MENDES, A. F. S.; CIDADE, L. C.; OTONI, W. C.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Role of auxins, polyamines and ethylene in root formation and growth in sweet orange. Biologia Plantarum, v. 55, n. 2, p. 375-378, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | MENDES, A. F. da S.; CIDADE, L. C.; MANZOLI, G. N.; OTONI, W. C.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Tissue culture parameters in sweet orange cultivars. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 8, p. 1093-1096, ago. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais. |
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| 5. | | COSTA, M. G. C.; MENDES, A. F. S.; CIDADE, L. C.; SOARES FILHO, W. dos S.; OTONI, W. C.; MOORE, G. A. Towards metabolic engineering of carotenoid content in sweet-orange (Citrus sinensis (L.) Osb.). In: XU, Z.; LI, J.; XUE, Y.; YANG, W. (Ed.). Biotechnology and sustainable agriculture 2006 and beyond. Heidelberg: Springer, 2007. p. 223-225. Edição do Proceedings of the 11th IAPTC&B Congress, Beijing, ago. 2006.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | COSTA, M. G. C.; GONÇALVES, L. P.; MARTINS, C. P. S.; PEDROSA, A. M.; PEREIRA, S. L. S.; OLIVEIRA, T. M.; CIDADE, L. C.; CAMILLO, L. R.; GESTEIRA, A. da S.; COELHO FILHO, M. A.; SOARES FILHO, W. dos S. Transcriptional regulation of drought response and tolerance in citrus and strategies for development of drought-tolerant transgenic rootstocks. In: INTERNATIONAL CITRUS CONGRESS, 2016, Foz do Iguaçu. Abstract book. Campinas: IAC; Londrina: IAPAR, 2016. p.149. S14-56.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | MARTINS, C. P. S.; NEVES, D. M.; CIDADE, L. C.; MENDES, A. F. S.; COELHO FILHO, M. A.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Functional analysis of a tonoplast intrinsic protein (TIP) gene involved in the response to osmotic stress in citrus. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [S.l.]: International Advanced Biology Consortium, 2013. Online. S03P01.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | CIDADE, L. C.; OLIVEIRA, T. M. de; MENDES, A. F. S.; MACEDO, A. F.; FLOH, E. I. S.; GESTEIRA, A. da S.; SOARES FILHO, W. dos S.; COSTA, M. G. C. Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco. Molecular Biology Report, v.39, p. 10201-10209, 2012| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | COSTA, M. G. C.; CIDADE, L. C.; OLIVEIRA, T. M. de; MARTINS, C. P. S.; MENDES, A. F. S.; PEREIRA, S. L. S.; PEDROSA, A. M.; GESTEIRA, A. da S.; GIRARDI, E. A.; COELHO FILHO, M. A.; SOARES FILHO, W. dos S.; MACHADO, M. A. Characterization of genes associated with two agronomically important traits in citrus: drought tolerance and fruit color. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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