Data/hora: 22/06/2024 02:29:53

Biblioteca(s): | Embrapa Meio Norte / UEP-Parnaíba.
Data corrente: | 15/04/1999
Data da última atualização: | 15/04/1999
Autoria: | CHRISTOU, P.; MCCABE, D. E.; SWAIN, W. F.
Título: | Stable transformation of soybean callus by DNA-coated gold particles.
Ano de publicação: | 1988
Fonte/Imprenta: | Plant Physiology, v.87, p.671-674, 1988.
Volume: | v.87
Páginas: | p.671-674
Idioma: | Inglês
Conteúdo: | Immature soybean (Glycine max L.) embryos from commercially important cultivars were the targets of rapidly accelerated, DNA-coated, gold particles. Protoplasts were prepared from these tissues and propagated in culture under selection conditions for the introduced neomycin phosphotransferase II gene. Kanamycin-resistant calli were obtained at a rate of approximately 10-5. Enzyme assays and Southern blot hybridization confirmed the expression of the foreign gene and its stable integration into the soybean genome. Our results show that particle acceleration can be used for the introduction of foreign DNA into the soybean genome and indicate the technique may be useful in the recovery of engineered plants by transformation of regenerable tissues.
Palavras-Chave: | Plant transformation; Soybean; Transforma?ao.
Thesagro: | DNA; Glycine Max; Soja.
Categoria do assunto: | --
Registro original: | Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)
Exemplares: | CPAMN-UEPP - (8598-1) - ADD - SP - -- -  -  - 1999.00015 - DIS