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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/12/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
EMERY, B. D. de; FURIAN, T. Q.; PILATTI, R. M.; CHITOLINA, G. Z.; BORGES, K. A.; SALLE, C. T. P.; MORAES, H. L. S. |
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Afiliação: |
Brunna D. de Emery, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Thales Q. Furian, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Roberta M. Pilatti, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Gabriela Z. Chitolina, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Karen A. Borges, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Carlos T. P. Salle, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS; Hamilton L. S. Moraes, Centro de Diagnóstico e Pesquisa Aviária - CDPA/Faculdade de Veterinária/Universidade Federal do Rio Grande do Sul - UFRGS. |
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Título: |
Evaluation of the biofilm formation capacity of Pasteurella multocida strains isolated from cases of fowl cholera and swine lungs and its relationship with pathogenicity. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1041-1048, outubro 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Avaliação da capacidade de formação de biofilme por cepas de Pasteurella multocida isoladas de casos de cólera aviária e de pulmões de suínos e sua relação com a patogenicidade. |
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Conteúdo: |
Pasteurella multocida is a Gram-negative bacillus that causes economic losses due to the development of respiratory diseases in several animal species. Among the mechanisms of virulence, the formation of biofilms is an important factor for bacterial survival in hostile environments. Studies of biofilm formation by P. multocida are needed because P. multocida is an important pathogen involved in respiratory infections. However, in contrast to other microorganisms, few studies of biofilm formation have examined P. multocida. Studies comparing the pathogenicity of microbial strains as a function of their biofilm production capacity are also rare. Consequently, the aim of this study was to evaluate the biofilm formation capacity of 94 P. multocida strains isolated from cases of fowl cholera and from swine lungs on polystyrene plates. The associations of the biofilm formation capacity with the pathogenicity index (PI) in vivo and with the presence of four genes (screened by PCR) of the tad locus (tadB, tadD, tadE and tadG), described as adhesion markers, were also determined. Strains from both animal origins were able to form biofilms. However, most of the specimens (52.13%) were classified as weak producers, and more than 40% of the strains of P. multocida (40.42%) did not produce biofilms. There was no significant difference (p>0.05) in the degree of biofilm production between the two sources of isolation. Of the analyzed strains, 56.52% contained all four genes (tadB, tadD, tadE and tadG). The PI arithmetic mean of the strains classified as non-biofilm producers was significantly different (p<0.05) from the PI of moderate-producer strains. The PI of specimens classified as weak biofilm producers also differed significantly (p<0.05) from that of the moderate-producer strains. The results indicate that even though the P. multocida strains isolated from cases of fowl cholera and swine lungs formed biofilms on polystyrene surfaces, adhesion was usually weak. The genes tadB, tadD, tadE and tadG were not significantly associated (p>0.05) with the production of biofilms and with the origin of a given strain. Finally, low virulence strains may suggest a higher biofilm formation capacity on polystyrene plates. MenosPasteurella multocida is a Gram-negative bacillus that causes economic losses due to the development of respiratory diseases in several animal species. Among the mechanisms of virulence, the formation of biofilms is an important factor for bacterial survival in hostile environments. Studies of biofilm formation by P. multocida are needed because P. multocida is an important pathogen involved in respiratory infections. However, in contrast to other microorganisms, few studies of biofilm formation have examined P. multocida. Studies comparing the pathogenicity of microbial strains as a function of their biofilm production capacity are also rare. Consequently, the aim of this study was to evaluate the biofilm formation capacity of 94 P. multocida strains isolated from cases of fowl cholera and from swine lungs on polystyrene plates. The associations of the biofilm formation capacity with the pathogenicity index (PI) in vivo and with the presence of four genes (screened by PCR) of the tad locus (tadB, tadD, tadE and tadG), described as adhesion markers, were also determined. Strains from both animal origins were able to form biofilms. However, most of the specimens (52.13%) were classified as weak producers, and more than 40% of the strains of P. multocida (40.42%) did not produce biofilms. There was no significant difference (p>0.05) in the degree of biofilm production between the two sources of isolation. Of the analyzed strains, 56.52% contained all four genes (tadB, tadD, ta... Mostrar Tudo |
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Palavras-Chave: |
Tad locus. |
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Thesagro: |
Biofilme; Pasteurella Multocida; Patogenicidade. |
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Thesaurus Nal: |
Biofilm; Pathogenicity. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169237/1/Evaluation-of-the-biofilm-formation-capacity.pdf
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Marc: |
LEADER 03346naa a2200277 a 4500
001 2082854
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aEMERY, B. D. de
245 $aEvaluation of the biofilm formation capacity of Pasteurella multocida strains isolated from cases of fowl cholera and swine lungs and its relationship with pathogenicity.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Avaliação da capacidade de formação de biofilme por cepas de Pasteurella multocida isoladas de casos de cólera aviária e de pulmões de suínos e sua relação com a patogenicidade.
520 $aPasteurella multocida is a Gram-negative bacillus that causes economic losses due to the development of respiratory diseases in several animal species. Among the mechanisms of virulence, the formation of biofilms is an important factor for bacterial survival in hostile environments. Studies of biofilm formation by P. multocida are needed because P. multocida is an important pathogen involved in respiratory infections. However, in contrast to other microorganisms, few studies of biofilm formation have examined P. multocida. Studies comparing the pathogenicity of microbial strains as a function of their biofilm production capacity are also rare. Consequently, the aim of this study was to evaluate the biofilm formation capacity of 94 P. multocida strains isolated from cases of fowl cholera and from swine lungs on polystyrene plates. The associations of the biofilm formation capacity with the pathogenicity index (PI) in vivo and with the presence of four genes (screened by PCR) of the tad locus (tadB, tadD, tadE and tadG), described as adhesion markers, were also determined. Strains from both animal origins were able to form biofilms. However, most of the specimens (52.13%) were classified as weak producers, and more than 40% of the strains of P. multocida (40.42%) did not produce biofilms. There was no significant difference (p>0.05) in the degree of biofilm production between the two sources of isolation. Of the analyzed strains, 56.52% contained all four genes (tadB, tadD, tadE and tadG). The PI arithmetic mean of the strains classified as non-biofilm producers was significantly different (p<0.05) from the PI of moderate-producer strains. The PI of specimens classified as weak biofilm producers also differed significantly (p<0.05) from that of the moderate-producer strains. The results indicate that even though the P. multocida strains isolated from cases of fowl cholera and swine lungs formed biofilms on polystyrene surfaces, adhesion was usually weak. The genes tadB, tadD, tadE and tadG were not significantly associated (p>0.05) with the production of biofilms and with the origin of a given strain. Finally, low virulence strains may suggest a higher biofilm formation capacity on polystyrene plates.
650 $aBiofilm
650 $aPathogenicity
650 $aBiofilme
650 $aPasteurella Multocida
650 $aPatogenicidade
653 $aTad locus
700 1 $aFURIAN, T. Q.
700 1 $aPILATTI, R. M.
700 1 $aCHITOLINA, G. Z.
700 1 $aBORGES, K. A.
700 1 $aSALLE, C. T. P.
700 1 $aMORAES, H. L. S.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n, 10, p. 1041-1048, outubro 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 2 | |
| 1. |  | EMERY, B. D. de; FURIAN, T. Q.; PILATTI, R. M.; CHITOLINA, G. Z.; BORGES, K. A.; SALLE, C. T. P.; MORAES, H. L. S. Evaluation of the biofilm formation capacity of Pasteurella multocida strains isolated from cases of fowl cholera and swine lungs and its relationship with pathogenicity. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n, 10, p. 1041-1048, outubro 2017. Título em português: Avaliação da capacidade de formação de biofilme por cepas de Pasteurella multocida isoladas de casos de cólera aviária e de pulmões de suínos e sua relação com a patogenicidade.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | CARVALHO, D.; MORAES, L. B. de; CHITOLINA, G. Z.; HERPICH, J. I.; OSÓRIO, F. S.; FALLAVENA, L. C. B.; MORAES, H. L. de S.; SALLE, C. T. P. Evaluation of thymic lymphocyte loss of broiler using Digital Analysis of the Lymphoid Depletion System (ADDL). Pesquisa Veterinária Brasileira, Brasília, DF, v.36, n. 7, p. 652-656, jul. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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