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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Florestas; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/02/1992 |
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Data da última atualização: |
18/04/2000 |
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Autoria: |
CHIN, H. F.; KRISHNAPILLAY, B.; ALANG, Z. C. |
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Título: |
Media for embryo culture of some tropical recalcitrant species. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Pertanika, Selangor, v.ll, n.3, p.357-363, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
In this study, the excised embryos of 11 recalcitrant species were studied to determine suitable media and cultural conditions for their development into whole plants. Murashige and Skoog's medium formulation modified slightly by the addition of 0.17 gl -1 NaH2PO4 was found to be a suitable basal medium for all the species studied. The basal medium supplemented with 2 gl-1 activated charcoal and 1.0 mgl-1 NAA or IAA in combination with 1 mgl-1 Kinetin, BAP or 2iP was found to be suitable for all the 11 species where the embryos developed normally to form whole plants. For some of the species, soaking the sterile excised embryos in an antioxidant (10 mgl-1 ascorbic acid and 5 mgl-1 citric acid) solution for 10-45 minutes prior to culture enhanced the normal development of embryos. |
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Palavras-Chave: |
Especie recalcitrante; media formulation; Semente recalcitrante. |
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Thesagro: |
Armazenamento; Cultura de Embrião; Germoplasma; Semente. |
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Thesaurus Nal: |
embryo culture; germplasm; recalcitrant seeds; recalcitrant species; seeds. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01565naa a2200289 a 4500
001 1303868
005 2000-04-18
008 1988 bl --- 0-- u #d
100 1 $aCHIN, H. F.
245 $aMedia for embryo culture of some tropical recalcitrant species.
260 $c1988
520 $aIn this study, the excised embryos of 11 recalcitrant species were studied to determine suitable media and cultural conditions for their development into whole plants. Murashige and Skoog's medium formulation modified slightly by the addition of 0.17 gl -1 NaH2PO4 was found to be a suitable basal medium for all the species studied. The basal medium supplemented with 2 gl-1 activated charcoal and 1.0 mgl-1 NAA or IAA in combination with 1 mgl-1 Kinetin, BAP or 2iP was found to be suitable for all the 11 species where the embryos developed normally to form whole plants. For some of the species, soaking the sterile excised embryos in an antioxidant (10 mgl-1 ascorbic acid and 5 mgl-1 citric acid) solution for 10-45 minutes prior to culture enhanced the normal development of embryos.
650 $aembryo culture
650 $agermplasm
650 $arecalcitrant seeds
650 $arecalcitrant species
650 $aseeds
650 $aArmazenamento
650 $aCultura de Embrião
650 $aGermoplasma
650 $aSemente
653 $aEspecie recalcitrante
653 $amedia formulation
653 $aSemente recalcitrante
700 1 $aKRISHNAPILLAY, B.
700 1 $aALANG, Z. C.
773 $tPertanika, Selangor$gv.ll, n.3, p.357-363, 1988.
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Registro original: |
Embrapa Florestas (CNPF) |
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