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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, C. F.; GUTIERREZ, D. L.; KREUZE, J. F.; ISKRA-CARUANA, M. L.; CHABANNES, M.; BARBOSA, A. C. O.; SANTOS, T. A.; SILVA, A. G. S.; SANTOS, R. M. F.; AMORIM, E. P.; OLIVEIRA, S. A. S. de; JESUS, O. N. de. |
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Afiliação: |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
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Título: |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n.3, 2019. |
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Idioma: |
Inglês |
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Notas: |
gmr18394 |
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Conteúdo: |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
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Thesagro: |
Melhoramento Vegetal. |
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Thesaurus Nal: |
Breeding and Genetic Improvement. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202165/1/-rapid-plant-dna-and-rna-extraction-protocol-using-bench-drill.pdf
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Marc: |
LEADER 02135naa a2200289 a 4500
001 2112332
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aFERREIRA, C. F.
245 $aRapid plant DNA and RNA extraction protocol using a bench drill Brief Note.$h[electronic resource]
260 $c2019
500 $agmr18394
520 $aPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.
650 $aBreeding and Genetic Improvement
650 $aMelhoramento Vegetal
700 1 $aGUTIERREZ, D. L.
700 1 $aKREUZE, J. F.
700 1 $aISKRA-CARUANA, M. L.
700 1 $aCHABANNES, M.
700 1 $aBARBOSA, A. C. O.
700 1 $aSANTOS, T. A.
700 1 $aSILVA, A. G. S.
700 1 $aSANTOS, R. M. F.
700 1 $aAMORIM, E. P.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aJESUS, O. N. de
773 $tGenetics and Molecular Research$gv. 18, n.3, 2019.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2002 |
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Data da última atualização: |
22/03/2002 |
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Autoria: |
CIAMPI, A. Y. |
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Afiliação: |
Embrapa Recursos Geneticos e Biotecnologia. |
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Título: |
DNA technologies for tropical trees-development of simple sequence repeat (SSR) markers. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
In: ENCONTRO LATINO-AMERICANO DE BIOTECNOLOGIA VEGETAL: REDBIO, 4., 2001, Goiania. Programa e resumos. [S.l.:s.n.], 2001. |
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Páginas: |
p.57. |
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Idioma: |
Português |
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Notas: |
Secao Conferencias. |
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Palavras-Chave: |
Arvore tropical; SSR; Tropical trees. |
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Thesagro: |
Biotecnologia; Marcador Genético. |
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Thesaurus NAL: |
biotechnology; genetic markers. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00669nam a2200205 a 4500
001 1180390
005 2002-03-22
008 2001 bl uuuu u01u1 u #d
100 1 $aCIAMPI, A. Y.
245 $aDNA technologies for tropical trees-development of simple sequence repeat (SSR) markers.
260 $aIn: ENCONTRO LATINO-AMERICANO DE BIOTECNOLOGIA VEGETAL: REDBIO, 4., 2001, Goiania. Programa e resumos. [S.l.:s.n.], 2001.$c2001
300 $ap.57.
500 $aSecao Conferencias.
650 $abiotechnology
650 $agenetic markers
650 $aBiotecnologia
650 $aMarcador Genético
653 $aArvore tropical
653 $aSSR
653 $aTropical trees
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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