Data/hora: 16/06/2024 20:42:07

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 23/09/2002
Data da última atualização: | 23/09/2002
Autoria: | YANKE, S. J.; CERI, H.; McALLISTER, T. A.; MORCK, D. W.; OLSON, M. E.
Título: | Serum immune response to Giardia duodenalis in experimentally infected lambs.
Ano de publicação: | 1998
Fonte/Imprenta: | Veterinary Parasitology, v.75, n.1, p.9-19, 1998.
Idioma: | Inglês
Conteúdo: | Twenty-three protozoan-free lambs were experimentally infected with Guardian duodenalis trophozoites at 6 weeks of age, while 24 control lambs were not challenged. Weekly blood samples were taken and faecal cyst counts monitored for 11 weeks foIlowing infection. Al experimentaIly infected Iambs remained infected throughout the 11 week study period and control animals remained free of the parasite. Giardia-specific serum IgM, IgG and IgA antibody titers were determined weekly in 10 infected and 10 controllambs by enzyine-linked-immunosorbent assay (ELISA). Western blot analysis of serum immunoglobulins to proteins derived from four different Giardia isolates (S2, WB, D3 and NF) was performed. Weekly mean control IgM, IgG and IgA titers did not change throughout the study. Infected lambs showed no difference in IgM titers between weeks O and 11, but IgG and IgA titers of infected Iambs differed from the preimmune (week O) serum titer at weeks 5,7,9,11 and weeks 5, 9 and 11 respectively. SDS polyacrylamide gel electrophoresis analysis showed homogeneity in the proteins of the four Giardia isolates. Antigenic proteins were aIso similar for alI four isolates; however, the proteins recognized by IgM, IgG and IgA antibodies differed. The weak immune response of Iambs to Giardia may account for the chronic nature of this disease in sheep.
Palavras-Chave: | Giardia duodenalis.
Thesagro: | Cordeiro; Giardíase; Imunologia; Ovino.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (14902-1) - ADC - AP - -- -  -  - v.75, n.1 - DIS