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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
30/11/2012 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOES, K. C. G. P. de; FISHER, M. L. de C.; CATTELAN, A. J.; NOGUEIRA, M. A.; CARVALHO, C. G. P. de; OLIVEIRA, A. L. M. de. |
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Afiliação: |
KELLY CAMPOS GUERRA PINHEIRO DE GOES, UEL; MARIA LUISA DE CASTRO FISHER, UEL; ALEXANDRE JOSE CATTELAN, CNPSO; MARCO ANTONIO NOGUEIRA, CNPSO; CLAUDIO GUILHERME PORTELA CARVALHO, CNPSO; ANDRÉ LUIZ MARTINEZ DE OLIVEIRA, UEL. |
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Título: |
Biochemical and molecular characterization of high population density bacteria isolated from sunflower. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Microbiology and Biotechnology, Seoul, v. 22, n. 4, p. 437-447, Feb. 2012. |
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DOI: |
10.4014/jmb.1109.09007 |
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Idioma: |
Inglês |
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Conteúdo: |
Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop. MenosNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the... 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Thesagro: |
Bactéria. |
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Categoria do assunto: |
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Marc: |
LEADER 05995naa a2200205 a 4500
001 1940892
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008 2012 bl uuuu u00u1 u #d
024 7 $a10.4014/jmb.1109.09007$2DOI
100 1 $aGOES, K. C. G. P. de
245 $aBiochemical and molecular characterization of high population density bacteria isolated from sunflower.$h[electronic resource]
260 $c2012
520 $aNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop.
650 $aBactéria
700 1 $aFISHER, M. L. de C.
700 1 $aCATTELAN, A. J.
700 1 $aNOGUEIRA, M. A.
700 1 $aCARVALHO, C. G. P. de
700 1 $aOLIVEIRA, A. L. M. de
773 $tJournal of Microbiology and Biotechnology, Seoul$gv. 22, n. 4, p. 437-447, Feb. 2012.
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| 16. |  | CATTELAN, A. J. Desenvolvimento e absorcao de nutrientes em plantas de soja inoculadas com bacterias promotoras do crescimento, em casa de vegetacao. In: SIMPOSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 3.; REUNIAO DE LABORATORIOS PARA RECOMENDACAO DE ESTIRPES DE RHIZOBIUM E BRADYRHIZOBIUM, 6., 1994, Londrina. Microbiologia do solo: desafios para o seculo XXI - anais. Londrina: IAPAR/EMBRAPA-CNPSo, 1995. p.387-392. Editado por Mariangela Hungria, Elcio Liborio Balota, Arnaldo Colozzi Filho, Diva de S. Andrade.| Biblioteca(s): Embrapa Soja. |
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